Medium and culture method of beauveria bassiana

The technology of Beauveria bassiana and culture medium is applied in the field of culture medium of Beauveria bassiana, and can solve the problems of weak virulence, long reproduction cycle, poor growth effect of Beauveria bassiana, etc.

Inactive Publication Date: 2017-06-30
SICHUAN ACAD OF AGRI SCI SERICULTURE INST +2
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of the existing Beauveria bassiana culture medium is relatively simple, and it is not good for promoting the growth of Beauveria bassiana. There are few designs for the growth process of Beauveria bassiana, and it i

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The culture medium of a kind of Beauveria bassiana described in the present embodiment comprises liquid culture medium and solid culture medium, wherein the component of liquid culture medium comprises 10 parts of glucose, 5 parts of yeast powder, potato juice by weight parts 7 parts, 12 parts of peptone, 8 parts of silkworm powder, 3 parts of potassium dihydrogen sulfate, 3 parts of cephalosporin, 60 parts of sterile water; the components of the solid medium include 10 parts of glucose and 10 parts of corn flour by weight. 8 parts of silkworm powder, 15 parts of lactose, 15 parts of wheat bran, 3 parts of cephalosporin.

[0020] Cultivation of Beauveria bassiana: inoculate the Beauveria bassiana species into the liquid medium, and culture at 24°C under shaking conditions for 3 days to obtain the Beauveria bassiana mycelium; Beauveria bassiana The mycelium of the fungus is inoculated in a solid medium and cultured at 26° C. for 5 days to obtain conidia of Beauveria bass...

Embodiment 2

[0023] The culture medium of a kind of Beauveria bassiana described in the present embodiment comprises liquid culture medium and solid culture medium, and wherein the component of liquid culture medium comprises glucose 5 parts, yeast powder 2 parts, potato juice by weight parts 3 parts, 5 parts of peptone, 5 parts of silkworm powder, 1 part of potassium dihydrogen sulfate, 1 part of cephalosporin, 20 parts of sterile water; the components of the solid medium include 5 parts of glucose and 5 parts of corn flour in parts by weight. 5 parts of silkworm powder, 10 parts of lactose, 10 parts of wheat bran, 1 part of cephalosporin.

[0024] Cultivation of Beauveria bassiana: Inoculate the Beauveria bassiana species into the liquid medium, and culture at 25°C under shaking conditions for 2 days to obtain the Beauveria bassiana mycelia; The mycelium of the fungus is inoculated in a solid medium and cultured at 25° C. for 4 days to obtain conidia of Beauveria bassiana.

[0025] In t...

Embodiment 3

[0027] The culture medium of a kind of Beauveria bassiana described in the present embodiment comprises liquid culture medium and solid culture medium, and wherein the component of liquid culture medium comprises glucose 15 parts, yeast powder 8 parts, potato juice by weight parts 10 parts, 20 parts of peptone, 10 parts of silkworm powder, 5 parts of potassium dihydrogen sulfate, 5 parts of cephalosporin, 80 parts of sterile water; the components of solid medium include 15 parts of glucose and 15 parts of corn flour by weight. 10 parts of silkworm powder, 20 parts of lactose, 20 parts of wheat bran, 5 parts of cephalosporin.

[0028] Cultivation of Beauveria bassiana: Inoculate the Beauveria bassiana species into the liquid medium, and culture at 24°C under shaking conditions for 4 days to obtain the Beauveria bassiana mycelia; The mycelium of the fungus is inoculated in a solid medium and cultured at 26° C. for 5 days to obtain conidia of Beauveria bassiana.

[0029] In the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a medium and culture method of beauveria bassiana. The medium includes a liquid medium and a solid medium. The liquid medium comprises the following components, by weight part, 5-15 parts of glucose, 2-8 parts of yeast powder, 3-10 parts of potato juice, 5-20 parts of peptone, 5-10 parts of silkworm powder, 1-5 parts of potassium dihydrogen phosphate, 1-5 parts of cephalosporin and 20-80 parts of sterile water. The solid medium comprises the following components, by weight part, 5-15 parts of glucose, 5-15 parts of corn flour, 5-10 parts of silkworm powder, 10-20 parts of lactose, 10-20 parts of wheat bran and 1-5 parts of cephalosporin. Beauveria bassiana conidia obtained from the medium have great power of invading host insects, are less polluted by other bacteria, and are high in success rate. The breeding period is short, and the quality of beauveria bassiana conidia is stable.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a medium for Beauveria bassiana and a method for cultivating Beauveria bassiana. Background technique [0002] Beauveria bassiana is an entomogenic fungus of the ascomycete class. The main species include Beauveria bassiana and Beauveria b. bassiana. It often produces conidia through asexual reproduction, and the hyphae have transverse septa and branches. Beauveria bassiana can invade 6 orders, 15 families, more than 200 kinds of insects and mites and reproduce in large numbers, and at the same time produce beauveria (non-ribosomal polypeptide toxoid), oosporin (benzoquinone toxoid) and calcium oxalate Crystallized, these substances can cause insect poisoning, disrupt metabolism and lead to death. Among them, Beauveria bassiana is an ascomycete fungus, which can be cultivated and produced industrially, and the molecular spores produced by it can be processed into m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 青学刚李文学陈宝瑞童文孙佩肖金树刘刚张友洪周安莲肖文福刘俊凤尹红王晓芬王皓
Owner SICHUAN ACAD OF AGRI SCI SERICULTURE INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap