Applications of Bacillus subtilis BAB-1 in prevention and control of powdery mildew of melons

A technology of Bacillus subtilis and BAB-1, which is applied in the fields of application, fungicides, chemicals for biological control, etc., can solve the problem that BAB-1 has not been found to prevent and control melon powdery mildew, etc. Simple, highly effective

Inactive Publication Date: 2017-07-14
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The patent "A Bacillus subtilis and its bacterial agent and its application" (CN101148650A) discloses that the Bacillus subtilis strain BAB-1 is effective in preventing and treating cotton blight, wheat sheath blight, tomato cinerea, c

Method used

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  • Applications of Bacillus subtilis BAB-1 in prevention and control of powdery mildew of melons

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation of embodiment 1 BAB-1 microbial bacterial agent

[0023] Follow the steps below:

[0024] (1) Strain activation: the Bacillus subtilis (Baci11us subtilis) strain BAB-1 (preservation number is CGMCC No.2099) stored at -80°C was placed on the LB plate medium (its composition and weight ratio are: pancreas 1g of peptone, 0.5g of yeast extract, 1g of sodium chloride, 15g of agar powder, 1000mL of water) for activation (30°C), pick a single colony on LB slant medium (its composition and weight ratio are the same as LB plate culture) base) at 32°C for 20 hours to obtain activated strains;

[0025] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, water 1000mL), in a 250mL Erlenmeyer flask Fill in 100mL of LB culture solution, and sterilize with high-pressure damp heat. After the temperature drops to room temperature, insert an ...

Embodiment 2

[0028] Example 2 BAB-1 Microbial Inoculum Contrast Test on Control Effect of Cucumber Powdery Mildew Potted Plants

[0029] (1) Test materials:

[0030] (1) Cucumber variety: Jinyou No. 1.

[0031] (2) Sphaerotheca fuliginea SF-1 conidia suspension. The initial strain came from Hebei Agricultural University, and the conidia suspension of cucumber powdery mildew pathogen used for inoculation was amplified from the initial strain in the Plant Disease and Biocontrol Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0032] (2) Test treatment:

[0033] (1) BAB-1 liquid preparation: a 100-fold water dilution of the BAB-1 liquid preparation prepared in Example 1.

[0034] (2) Blank control: clear water.

[0035] (3) Test method:

[0036] This experiment was carried out in the plant disease and biocontrol laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. Cultivate Jinyou No. 1...

Embodiment 3

[0040] Example 3 BAB-1 Microbial Inoculum Contrast Test on Control Effect of Cucumber Powdery Mildew Potted Plants

[0041] (1) Test materials:

[0042] (1) Cucumber variety: Jinyou No. 1.

[0043] (2) Cucumber powdery mildew pathogen (Sphaerotheca fuliginea) SF-1 conidia suspension (same as Example 2).

[0044] (2) Test treatment:

[0045] (1) BAB-1 liquid preparation: 100 times water dilution of the BAB-1 liquid preparation prepared in Example 1;

[0046] (2) Blank control: clear water.

[0047] (3) Test method:

[0048] This experiment was carried out in the plant disease and biocontrol laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. Cultivate Jinyou No. 1 cucumber seedlings in a nutrient bowl with a diameter of 6 cm. The seedling substrate is vermiculite. Two seeds are sown in each nutrient bowl, and one strong seedling is retained after emergence. When the cucumber seedlings grew two true leaves, they were transpl...

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Abstract

The invention discloses applications of Bacillus subtilis BAB-1 in prevention and control of powdery mildew of melons, and belongs to the field of biological prevention and control. The invention further discloses applications of a BAB-1-containing microbial agent in prevention and control of powdery mildew of melons. According to the present invention, the efficacy of the Bacillus subtilis BAB-1 on powdery mildew of melons is high, wherein the average prevention and control effect is more than 85.0% so as to provide the effective prevention and control method for powdery mildew of melons; by using the Bacillus subtilis BAB-1 to prevent and control powdery mildew of melons, drug resistance is not easily generated, and the efficacy lasting period is good; and the microbial agent does not produce the environmental pollution problem, and has characteristics of simple preparation, simple use, and low cost.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to the application of bacillus subtilis strain BAB-1 in preventing and treating melon powdery mildew. Background technique [0002] Melon powdery mildew is one of the most important leaf diseases in melon production. The disease is mainly caused by two fungi, Erysiphe cichoracearumhe and Sphaerotheca fuliginea. (Sphaerotheca fuliginea) predominately. Cucumber powdery mildew mainly harms crops such as cucumber, melon, zucchini, pumpkin and bitter gourd, especially cucumber and melon are the main vegetables and fruits planted in greenhouses in northern my country. The occurrence of powdery mildew seriously affects the yield and quality of cucumber and melon; usually due to Cucumber or melon production reduction caused by powdery mildew is about 10%, and it is as high as about 30% in popular years. The pathogen can infect melon plants from the seedling stage to the adult ...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P3/00
CPCA01N63/00
Inventor 张晓云马平李社增鹿秀云郭庆港
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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