Applications of Bacillus subtilis BAB-1 in prevention and control of powdery mildew of melons
A technology of Bacillus subtilis and BAB-1, which is applied in the fields of application, fungicides, chemicals for biological control, etc., can solve the problem that BAB-1 has not been found to prevent and control melon powdery mildew, etc. Simple, highly effective
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Embodiment 1
[0022] The preparation of embodiment 1 BAB-1 microbial bacterial agent
[0023] Follow the steps below:
[0024] (1) Strain activation: the Bacillus subtilis (Baci11us subtilis) strain BAB-1 (preservation number is CGMCC No.2099) stored at -80°C was placed on the LB plate medium (its composition and weight ratio are: pancreas 1g of peptone, 0.5g of yeast extract, 1g of sodium chloride, 15g of agar powder, 1000mL of water) for activation (30°C), pick a single colony on LB slant medium (its composition and weight ratio are the same as LB plate culture) base) at 32°C for 20 hours to obtain activated strains;
[0025] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, water 1000mL), in a 250mL Erlenmeyer flask Fill in 100mL of LB culture solution, and sterilize with high-pressure damp heat. After the temperature drops to room temperature, insert an ...
Embodiment 2
[0028] Example 2 BAB-1 Microbial Inoculum Contrast Test on Control Effect of Cucumber Powdery Mildew Potted Plants
[0029] (1) Test materials:
[0030] (1) Cucumber variety: Jinyou No. 1.
[0031] (2) Sphaerotheca fuliginea SF-1 conidia suspension. The initial strain came from Hebei Agricultural University, and the conidia suspension of cucumber powdery mildew pathogen used for inoculation was amplified from the initial strain in the Plant Disease and Biocontrol Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.
[0032] (2) Test treatment:
[0033] (1) BAB-1 liquid preparation: a 100-fold water dilution of the BAB-1 liquid preparation prepared in Example 1.
[0034] (2) Blank control: clear water.
[0035] (3) Test method:
[0036] This experiment was carried out in the plant disease and biocontrol laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. Cultivate Jinyou No. 1...
Embodiment 3
[0040] Example 3 BAB-1 Microbial Inoculum Contrast Test on Control Effect of Cucumber Powdery Mildew Potted Plants
[0041] (1) Test materials:
[0042] (1) Cucumber variety: Jinyou No. 1.
[0043] (2) Cucumber powdery mildew pathogen (Sphaerotheca fuliginea) SF-1 conidia suspension (same as Example 2).
[0044] (2) Test treatment:
[0045] (1) BAB-1 liquid preparation: 100 times water dilution of the BAB-1 liquid preparation prepared in Example 1;
[0046] (2) Blank control: clear water.
[0047] (3) Test method:
[0048] This experiment was carried out in the plant disease and biocontrol laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. Cultivate Jinyou No. 1 cucumber seedlings in a nutrient bowl with a diameter of 6 cm. The seedling substrate is vermiculite. Two seeds are sown in each nutrient bowl, and one strong seedling is retained after emergence. When the cucumber seedlings grew two true leaves, they were transpl...
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