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Preparation method for high-adaptability nitrifying bacterial agent

A technology of nitrifying bacteria agent and applicability, which is applied in the field of preparation of highly applicable nitrifying bacteria agents, can solve the problems of slow enrichment speed of nitrifying bacteria, long enrichment time, and insignificant strengthening effect, etc. Nitrification function, high similarity effect

Active Publication Date: 2017-07-14
YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taking the effluent ammonia nitrogen less than 5mg / L as the standard for further increasing the influent ammonia nitrogen concentration, the enrichment speed of nitrifying bacteria is slow and the enrichment time is long
[0007] In addition, the currently developed nitrifying bacteria agents are mainly used in urban sewage treatment plants, and the strengthening effect is not significant when improving the nitrification function of the biological treatment system for industrial wastewater treatment
Therefore, it is necessary to develop a method for rapidly enriching nitrifying bacteria, so that the enriched nitrifying bacteria have the characteristics of high nitrifying activity and rich microbial composition, which can restore and improve the nitrifying function of different wastewater biological treatment systems, and make up for the existing The problems of poor dosing effect, large dosage and repeated dosing of nitrifying bacteria agents have important practical significance for improving the ammonia nitrogen removal effect of various sewage biological treatment systems

Method used

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  • Preparation method for high-adaptability nitrifying bacterial agent
  • Preparation method for high-adaptability nitrifying bacterial agent
  • Preparation method for high-adaptability nitrifying bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] In the preparation method of nitrifying bacteria agent, the method for improving the ammonia nitrogen load is as follows:

[0073] Add ammonium sulfate to tap water to provide inorganic ammonia nitrogen, dipotassium hydrogen phosphate and potassium dihydrogen phosphate buffer to provide phosphorus source, and alkalinity regulator uses sodium bicarbonate as nitrifying bacteria culture solution. The nitrification activity of the nitrifying bacteria agent is provided by increasing the ammonia nitrogen load in the culture medium step by step, and the ammonia nitrogen load is from 0.3kg-N / (m 3 d) at 0.1kg-N / (m 3 ·d) The range is increased to 0.6kg-N / (m 3 After d), use 0.8kg-N / (m 3 The range of d) is increased to 1.4kg-N / (m 3 d), the effluent ammonia nitrogen rises rapidly, reducing the ammonia nitrogen load to 1.3kg-N / (m 3 After d), the effluent ammonia nitrogen is gradually reduced to 50mg / L. The above stage does not carry out mud discharge. The nitrification activity...

Embodiment 2

[0075] In the preparation method of nitrifying bacteria agent, ammonia nitrogen load and SRT change method are as follows:

[0076] Select a sewage treatment plant with a daily treatment capacity of more than 100,000 tons / d in the Yangtze River Delta region. The proportion of industrial water in the water is about 50%. The effluent treated by the secondary settling tank and the pig biogas slurry (the water quality of the raw sewage water, effluent water and pig biogas slurry are listed in the following table 1) are mixed according to the volume percentage of 70:25:5 and used as the nitrifying bacteria culture solution . Ammonium sulfate is added to the culture medium to provide inorganic ammonia nitrogen, dipotassium hydrogen phosphate and potassium dihydrogen phosphate buffer pair provide phosphorus source, and sodium bicarbonate is used as alkalinity regulator. Intermittent mud discharge is carried out while increasing the ammonia nitrogen load in the culture medium step by...

Embodiment 3

[0081] The preparation method of nitrifying bacteria agent is as follows:

[0082] Select a sewage treatment plant with a daily treatment capacity of more than 100,000 tons / d in the Yangtze River Delta region. The proportion of industrial water in the water is about 50%. The effluent treated by the secondary settling tank, and the pig biogas slurry (the water quality of the raw sewage water, effluent water and pig biogas slurry are listed in the above table 1) are mixed according to the volume percentage of 70:25:5 and used as the nitrifying bacteria culture solution . Ammonium sulfate is added to the culture medium to provide inorganic ammonia nitrogen, dipotassium hydrogen phosphate and potassium dihydrogen phosphate buffer pair provide phosphorus source, and sodium bicarbonate is used as alkalinity regulator. Increase the ammonia nitrogen load (ammonia nitrogen load from 0.65kg-N / (m 3 ·d) at 0.4~0.5kg-N / (m 3 The range of d) is increased by 1.5kg-N / (m 3 ·d), after that w...

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Abstract

The invention discloses a preparation method for a high-adaptability nitrifying bacterial agent. The preparation method comprises the steps of: 1) inoculating active sludge on a membrane biological reactor, and continuously feeding a culture solution, composed of sewage, inorganic ammonia nitrogen, a phosphate buffer solution and an alkalinity regulator, into the membrane biological reactor; (2) firstly, setting SRT as non-sludge discharge status, and controlling the ammonia nitrogen volume loading of the membrane biological reactor to be lower than 1.5 kg-N / (m<3>*d), and then stepwise increasing the ammonia nitrogen loading until a maximum value; and 3) finally determining minimum value of the SRT and under the minimum value of the SRT, continuously discharging the nitrifying bacterial agent. The preparation method can produce nitrifying bacteria, which are homologous with the bacteria from a sewage treatment plant, within a short time. The nitrifying bacteria have high degradation activity on ammonia nitrogen, have good adaptability and are low in addition frequency.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a preparation method of a highly applicable nitrifying bacteria agent. Background technique [0002] In recent years, with the improvement of discharge standards of sewage treatment plants and the control of the total amount of ammonia nitrogen, the stable and efficient removal of ammonia nitrogen is the key to reducing ammonia nitrogen emissions. The removal of ammonia nitrogen mainly depends on nitrifying bacteria, which grow slowly and are sensitive to dissolved oxygen, water temperature, and toxic substances, which lead to the breakdown of the nitrification function of the biological treatment system. [0003] The use of nitrifying bacteria can greatly shorten the start-up time and ammonia nitrogen removal capacity of the biological treatment system, improve the impact resistance of the nitrification functional unit and the ammonia nitrogen removal effect under low t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16C02F101/20C02F101/30C02F103/20
CPCC02F3/34C02F2101/16C02F2101/20C02F2101/30C02F2103/20C02F2209/44C12N1/20
Inventor 盛晓琳刘锐陈吕军
Owner YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG
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