Method for culturing pancreatic cancer cell under anaerobic environment

Abstract

The invention discloses a method for culturing pancreatic cancer cells under anaerobic environment. The method comprises the following step: by simulating a true growing environment of pancreatic cancer in a human body, culturing three common pancreatic cancer cells under anaerobic environment, wherein the cancer cells obtained through culturing are more closer to those growing under the true conditions, thus providing good base materials for studying the experiment accuracy and leading the acquired cancer cell material to have true persuasion in the following scientific experiments.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for cultivating pancreatic cancer cells in a hypoxic environment. Background technique [0002] Pancreatic cancer has a high degree of malignancy and a 5-year survival rate of less than 5%. It is known as the king of cancer. Most solid tumors are formed by the continuous proliferation of tumor cells, and the tumor and its surrounding tissues are often very rich in blood supply. The biological characteristics of tumors rich in blood supply are also the main reason for their rapid growth, and it also provides us with ideas for fighting tumors. After doctors block the blood supply of tumor tissue through various means such as drugs and surgery, they can often "starve" the tumor to get a good therapeutic effect. However, this series of treatment measures seems to be less effective in the face of pancreatic cancer, and has failed to significantly prolong the survival time ...

AI Technical Summary

Problems solved by technology

In this unique tumor microenvironment, not only is it difficult for antitumor drugs to reach the tumor through blood vessels, but it also limits the recognition and removal of tumor cells by normal immune cells. In addition, the commonly used "starvation therapy" will also be eclipsed, because the pancreas Cancer is not afraid of "hunger"

[0004] There is a class of hypoxia-inducible factors in the human body. Under normal circumstances, they are continuously degraded and maintained at low levels for a long time. However, studies have found that hypoxic microenvironments appear in many tumor tissues. However, the cultivation of pancreatic cancer cells is still It is a conventional culture method, and the cancer cells obtained in this formula are likely to be different from the cancer cells produced in the real hypoxic microenvironment

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