Method for direct induction of cluster buds from Millettia specisoa seeds and rapid breeding of seedlings

A Niu Dali and seed technology is applied in the field of Niu Dali seeds directly inducing from buds and quickly breeding seedlings, which can solve the problems of low seed germination rate and irregular germination, and achieve the solution of irregular germination, neat seedlings, and increased lignification. degree of effect

Inactive Publication Date: 2017-07-21
广西蚕越农业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, Niu Dali seeds and seedlings on the market are all carried out on a small scale, coupled with the low germination rate and irregular germination, it is difficult to meet the large-scale demand of the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] The method for directly inducing buds and rapidly breeding seedlings from Niu Dali seeds comprises,

[0020] The steps of primary culture are as follows: Cut the semi-lignified stems of Niu Dali, remove the leaves, cut into 4cm long stems, rinse them with water, sterilize them by ultrasonic for 8 minutes, rinse them with sterile water 5 times, cut off the incisions at both ends, Cut into 1cm-long stems with buds, inoculate them into the first-generation culture medium, cultivate in the dark for 7 days, the axillary buds germinate, and then culture in low light for 25 days, the axillary buds grow to 1cm, transfer to strong light for 30 days, and the axillary buds grow to 1.5cm long A single sprout without leaf differentiation.

[0021] The steps for the proliferation of buds are as follows: cut the stems of the sprouts grown in the primary culture into 1.5 long stems and place them horizontally in the bud proliferation medium. After 28 days of cultivation, 5 buds will ge...

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PUM

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Abstract

The invention relates to a method for directly inducing from buds and rapidly breeding seedlings of Niu Dali seeds, including the preparation of culture medium, primary culture, multiplication and cultivation from buds, further cultivation of strong seedlings, and rapid breeding and cultivation outside the bottle. The method for directly inducing buds from seeds and rapidly breeding seedlings provided by the invention has the advantages of neat germination, robust seedlings, high seedling success rate, short seedling time, low seedling cost and the like.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for directly inducing buds from seeds of Niu Dali and rapidly breeding seedlings. Background technique [0002] Niu Dali is the root of the beautiful Twig bean genus, which has the effects of relaxing tendons and activating collaterals, clearing away heat and relieving cough, nourishing the lungs and nourishing the kidneys, etc. It is distributed in Guangxi, Guangdong, Hainan, Taiwan, Guizhou and Southeast Asia. medicinal and edible plants. In recent years, due to the continuous increase in sales of various Chinese patent medicines such as Zhuangyao Jianshen Pills and Qiangli Fitness Capsules produced with Niu Dali as the main raw material, the demand for raw materials has also increased sharply. For endangered breeds, the artificial cultivation of cattle has become an inevitable trend. At present, the planting area of ​​Niu Dali is about 30,000 m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G9/10A01G1/00
CPCA01H4/001A01G22/00A01G24/00A01H4/008
Inventor 乐彧凯唐上富
Owner 广西蚕越农业有限责任公司
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