Skin whitening and spot fading composition and application thereof
A composition and a lightening technology, applied in the field of whitening and lightening compositions, can solve the problems of increasing chromosomal aberrations in ovarian cells, producing cytotoxic effects, chronic skin disfigurement, etc. simple craftsmanship
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[0027] The preparation method of the above-mentioned Cackle Leaf Extract is as follows: take the Cackle Leaf, crush it into coarse powder, add 5 times the amount of deionized water and decoct it for 500 minutes, repeat 3 times, combine the decoction, filter, and concentrate under normal pressure to a paste; take the above paste, add 4 times the amount of 80% ethanol and stir evenly, add sodium hydroxide solution dropwise to adjust the pH to 8, and keep it closed overnight; filter the feed liquid under reduced pressure, and recover the ethanol from the filtrate until it is alcohol-free Taste, refrigerated at 4°C for 24 hours, and then the filtrate was concentrated under normal pressure to obtain the extract of the leaves of Kackler off the coast.
[0028] The preparation method of kudzu root extract is as follows: take fresh kudzu root, wash and dry, crush into coarse particles, decoct the coarse particles with water for 3 hours each time, combine the two filtrates, let stand fo...
Embodiment 1
[0045] Embodiment 1 whitening plant composition (self-made); 95% ethanol (Sinopharm Group Chemical Reagent Co., Ltd.); DPPH (Tokyo Chemical Co., Ltd., analytically pure); AB204-E electronic analytical balance (METTLE TOLEDO); UV- 1600 UV spectrophotometer (METTLE TOLEDO).
[0046] DPPH appears dark purple in absolute ethanol and ethanol-water systems, and has maximum absorption at 517nm and 525nm, respectively. The free radical scavenger can make the purple fade gradually, and the content of DPPH and the scavenging ability of the reactive free radical scavenger to DPPH are measured according to the change of absorbance.
[0047] Accurately weigh 3.00mg of DPPH, dissolve it with 95% ethanol and set the volume to 100ml, the solubility of DPPH is 20mg / L, take 4ml of DPPH standard solution, put it in a test tube, add 1ml of whitening and lightening plant composition solution, and let it stand for 30min , Absorbance A was measured at 517 nm. Determination of DPPH clearance rate a...
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