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Transfer RNA fragments and their applications

A kind of fragment, the technology of SEQIDNO.14-SEQIDNO.16, is used in transfer RNA fragment and its application field

Active Publication Date: 2020-08-14
上海市第六人民医院东院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Whether such small RNAs are involved in angiogenesis has not been reported yet

Method used

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  • Transfer RNA fragments and their applications
  • Transfer RNA fragments and their applications
  • Transfer RNA fragments and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] tRNA-Gly fragment mimics inhibit pancreatic cancer growth

[0023] The pancreatic cancer cell suspension was subcutaneously injected into the groin of nude mice, and randomly divided into 2 groups, with 5 mice in each group. Simultaneously, the tRNA-Gly fragment mimic sequence was injected through the tail vein, injected every other day until the 30th day, and all nude mice were sacrificed. Rats, tumors were removed, examined and measured. The results showed that the injection of tRNA-Gly fragment analogs significantly inhibited the tumor size of pancreatic cancer, see Figure 4 , Figure 4 The three tumor bodies in the middle and upper part are the situation of the control injection group, and the three tumor bodies in the lower part are the situation of the tRNA-Gly fragment mimic injection group. The sequence of the tRNA-Gly fragment mimic is GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT (SEQ ID NO. 12).

Embodiment 2

[0025] Targeted injection of tRNA-Gly fragment antisense sequence promotes angiogenesis in ischemic lower extremity

[0026] Construct 10 mice lower limb ischemia models and randomly divide them into two groups, A and B. Group A was injected with control nonsense sequence RNA, and group B was injected with tRNA-Gly fragment antisense sequence. Doppler was used to observe the restoration of blood flow. The results showed that compared with the control group, the blood supply of the injection tRNA-Gly fragment antisense sequence group was significantly restored, see Figure 5 , Figure 6 , Figure 5 is the case of the control injection group, Figure 6 This is the case of the tRNA-Gly fragment antisense sequence injection group. It indicated that injection of antisense sequence of tRNA-Gly fragment could promote angiogenesis in ischemic lower extremity. The antisense sequence of the tRNA-Gly fragment is AGGCGAGAATTCTACCACTGAACCACCAATGC (SEQ ID NO.15).

[0027]

[0028] ...

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PUM

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Abstract

The invention discloses multiple transfer RNA fragments which derive from the following fragments with full-length sequences and containing 20-55 nucleotides: tRNA-val, tRNA-gly, tRNA-glu or tRNA-his, wherein the full-length sequence of tRNA-val is shown in SEQ ID NO 1, the full-length sequence of tRNA-gly is shown in SEQ ID NO 2, the full-length sequence of tRNA-glu is shown in SEQ ID NO 3, and the full-length sequence of tRNA-his is shown in SEQ ID NO 4. The transfer RNA fragments can be used for treating tumors and regulating angiogenesis.

Description

technical field [0001] The invention relates to bioengineering technology, in particular to transfer RNA segment and its application. Background technique [0002] Angiogenesis refers to the biological process in which endothelial cells proliferate, migrate and remodel to form new blood vessels on the basis of existing blood vessels. Pathophysiological processes such as blood diseases and tumor growth and invasion. In-depth study of the regulatory mechanism of angiogenesis has important guiding significance for promoting tissue repair, inhibiting tumor growth and migration. Studies have shown that a variety of non-coding RNAs, including miRNAs, piRNAs, and lncRNAs, are involved in the regulation of angiogenesis. [0003] Transfer RNA (tRNA) is a kind of small molecule RNA with a length of 73-94 nucleotides, which is an indispensable part of cell activities. They are mainly responsible for translating messenger RNA (mRNA) sequences into amino acid sequences to synthesize pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11A61K31/7105A61P35/00A61P9/10
CPCA61K9/0019A61K9/10A61K31/7105C12N15/11C12N2310/10C12N2320/30
Inventor 邓志锋李青汪泱胡斌牛鑫
Owner 上海市第六人民医院东院
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