QTL (Quantitative Trait Locus) relevant to soybean salt tolerance, SNP (Single Nucleotide Polymorphism) molecular marker and application of QTL and SNP molecular marker
A molecular marker and salt tolerance technology, applied in the field of molecular biology, can solve the problem that the mechanism of action and application research have not been fully carried out, and the breeding has not yet been formed, and achieve the effect of accelerating the improvement process and improving the selection efficiency.
Inactive Publication Date: 2017-07-25
JILIN ACAD OF AGRI SCI
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Problems solved by technology
However, at present, the research on the mechanism and application of QTL related to salt tolerance has not been fully carried out, and the situation that can be effectively used in breeding has not yet been formed.
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[0022] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. The experimental methods in the following examples, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.
[0023] The present invention provides a QTL related to soybean salt tolerance. The QTL related to soybean salt tolerance is located in the M7322-M7321-M7306 interval of chromosome 2, and is located by SNP molecular markers M7322, M7321 and M7306. Specifically, it is obtained as follows:
[0024] 1. Construction of genetic population
[0025] Sexual crosses were carried out with Yiqianli, a local soybean variety with low salt tolerance, as the female parent and Changling wild soybean with high salt tolerance as the male parent, and F 1 ...
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The invention discloses QTL (Quantitative Trait Locus) relevant to soybean salt tolerance, wherein the OTL is located in an interval of M7322-M7321-M7306 on chromosome 2 and located by M7322, M7321 and M7306 as SNP (Single Nucleotide Polymorphism) molecular markers. The molecular markers tightly linked to the QTL are M7322, M7321 and M7306, a nucleotide sequence of M7322 is shown as SEQ ID NO. 1, a nucleotide sequence of M7321 is shown as SEQ ID NO. 2, and a nucleotide sequence of M7306 is shown as SEQ ID NO. 3. The invention also discloses application of the QTL relevant to the soybean salt tolerance. The QTL and the molecular marker can be used for marker-assisted selection in breeding, remarkably improve the selection efficiency of a salt tolerance material and provide a most economic and effective new way of molecular breeding to further enrich a regulatory network of the soybean salt tolerance.
Description
technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a QTL and SNP molecular marker related to soybean salt tolerance and application thereof. Background technique [0002] In recent years, about 10% of the cultivated land in my country has been salinized to varying degrees. The precipitation in the main grain-producing areas in northern my country is very small, resulting in the rise of saline groundwater, and the salt stays in the soil; coastal areas are eroded by seawater, and the soil Salinization is also expanding. Salt stress has a great impact on the growth and yield of soybean. According to the normal growth ratio of plants under high salt stress, the salt stress tolerance of plants is graded. The grade results show that cultivated soybean is a moderately salt-tolerant crop. The salt tolerance of soybean depends on its own tolerance to salt stress, and the tolerance of different varieties is different. ...
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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 邱红梅王跃强王丽王洋马晓萍高淑芹姚丽颖侯云龙陈健胡金海
Owner JILIN ACAD OF AGRI SCI