A Pichia fermentation method for improving the expression of insulin and its analogue precursors

Abstract

The invention relates to the field of biological pharmacy, in particular to a pichia pastoris fermenting method for improving precursor expression of insulin and analogue thereof. The fermenting culture comprises the following stages of an initial growth phase, a glycerin supplementing phase and a methanol inducing phase. The method has the advantages that by controlling a formula of an initial culture medium, and the nitrogen source type and carbon source supplementing type, and continuously or semi-continuously supplementing a small amount of microorganisms or plant-source organic nitrogen sources in the middle and later period of fermenting, the fermenting cycle is extended; while the quality of the target product is guaranteed, the output is increased by not less than 30%.

Description

technical field

[0001] The invention relates to the field of biopharmaceuticals, in particular to a Pichia yeast fermentation method for improving the expression of insulin and its analog precursors. Background technique

[0002] Diabetes is a common endocrine and metabolic disease. In recent years, the prevalence of diabetes has been increasing rapidly all over the world. In 2015, the number of adult diabetic patients in the world reached 415 million, among which the number of diabetic patients in China exceeded 100 million. Diabetes has become another important chronic non-communicable disease that seriously endangers people's health after cardiovascular and cerebrovascular diseases and tumors. The acute and chronic complications of diabetes, especially the complications of chronic diseases, involve multiple organs, causing disability and high mortality, seriously affecting the physical and mental health of patients, and bringing heavy burdens to individuals, families an...

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