Medical acellular dermal matrix and preparation method thereof

A decellularized dermis and matrix technology, applied in the field of medical acellular dermal matrix and its preparation, can solve the problems of large basement membrane damage, short processing time, and occupation of man-hours, so as to increase shrinkage temperature, improve production efficiency, and save production costs Effect

Inactive Publication Date: 2017-08-11
HENAN HUIBO MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are several methods for the preparation of acellular dermal matrix: 1. Dispase II-Triton method: This method removes cells completely, but it damages the basement membrane greatly, which is not conducive to the adhesion and growth of epithelial cells; 2. Hypertonicity Salt-SDS method: This method retains the basement membrane intact, but the content of fibronectin and elastin in the dermis is high, so it may have high immunogenicity; 3. Hypertonic salt-sodium hydroxide erosion method: the The method uses hypertonic salt to remove the epidermis, and at the same time uses the water absorption effect of alkali ions in sodium hydroxide to deprive the tissue cells of water and dehydrate the cells. This method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0039] Example 1

[0040] A method for preparing medical acellular dermal matrix, including the following steps:

[0041] 1) After removing the meat from the fresh pigskin skin, soak it in a 0.5% benzalkonium bromide solution for 30 minutes;

[0042] 2) Immerse the animal skin after step 1) disinfection in the APG1214 solution with a concentration of 1%, and wash it 3 times, 2 hours each time;

[0043] 3) Soak the cleaned animal skin in step 2) in a sodium chloride solution with a concentration of 1mol / L for 24 hours to break the hemidesmosome between epithelial cells and connective tissue, tear off the epidermis, and wait for treatment Dermal skin

[0044] 4) Put the to-be-treated dermal skin sheet prepared in step 3) into a mixed solution containing 4% sodium hydroxide and 2% hydrogen peroxide and soak for 18 hours;

[0045] 5) Cut the dermal skins processed in step 4) to length * width * thickness = 10cm~80cm*10cm~80cm*0.2mm~1.0mm size skin;

[0046] 6) Soak the cut skin piece in step...

Example Embodiment

[0049] Example 2

[0050] A method for preparing medical acellular dermal matrix, including the following steps:

[0051] 1) After removing the meat from the fresh pigskin skin, soak it in a 0.8% peroxyacetic acid solution for 45 minutes;

[0052] 2) Immerse the sterilized animal skin in step 1) in APG1216 solution with a concentration of 0.1%, and wash it 3 times, each for 1 hour;

[0053] 3) Immerse the cleaned animal skin in step 2) in a 0.8mol / L sodium chloride solution for 12 hours to break the hemidesmosome between epithelial cells and connective tissue, tear off the epidermis, and wait Processing dermal skins;

[0054] 4) Put the dermal skin sheet to be processed prepared in step 3) into a mixed solution containing 2% sodium hydroxide and 0.5% hydrogen peroxide, and soak for 24 hours;

[0055] 5) Cut the dermal skins processed in step 4) to length * width * thickness = 10cm~80cm*10cm~80cm*0.2mm~1.0mm size skin;

[0056] 6) Soak the cut skin piece in step 5) in a solution containin...

Example Embodiment

[0059] Example 3

[0060] A method for preparing medical acellular dermal matrix, including the following steps:

[0061] 1) After removing the meat from the fresh pigskin skin, soak it in a 1.0% benzalkonium bromide solution for 60 minutes;

[0062] 2) Soak the animal skin after disinfection in step 1) in a 2.0% sodium lauryl sulfate solution, and wash it twice for 0.5 hours each time;

[0063] 3) Soak the cleaned animal skin in step 2) in a sodium chloride solution with a concentration of 2.0mol / L for 4 hours to break the hemidesmosomes between epithelial cells and connective tissues, tear off the epidermis, and wait Processing dermal skins;

[0064] 4) Put the dermal skin sheet to be processed prepared in step 3) into a mixed solution containing 6% potassium hydroxide and 5% hydrogen peroxide, and soak for 3 hours;

[0065] 5) Cut the dermal skins processed in step 4) to length * width * thickness = 10cm~80cm*10cm~80cm*0.2mm~1.0mm size skin;

[0066] 6) Soak the cut skin piece in step...

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Abstract

The invention relates to the field of medical biomaterials, and concretely relates to a medical acellular dermal matrix and a preparation method thereof. The preparation method comprises the following steps: fleshing animal skins, sequentially carrying out disinfecting, cleaning and hypertonic saline treatment, and immersing the treated animal skins in an alkali liquid and hydrogen peroxide mixed solution; and carrying out enzyme digestion treatment on the animal skins in a trypsin solution, and crosslinking collagens by using a crosslinking agent. The treatment of a hypertonic saline treated dermal sheet with the alkali liquid and hydrogen peroxide mixed solution makes protein molecules in the dermal sheet expanded and loosened and hair directly removed, so the manual hair removal process is omitted, the process flow is simplified, the production efficiency is increased, the protection cost is saved, and the used reagents are nontoxic and do not cause environment pollution. The crosslinking treatment is carried out after the enzyme digestion, so destroys of excess digestion the structure and the arrangement mode of the collagens are avoided, and the toughness, the elasticity and the flexibility of the product are improved.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a medical acellular dermal matrix and a preparation method thereof. Background technique [0002] For patients with extensive burns or trauma, skin grafting is the best treatment method, but it is almost impossible to use autologous full-thickness skin grafting for extremely large burn wounds, while autologous microskin or thin skin grafts require dermal scaffolds. The acellular dermis made from human cadaver skin is the most ideal as a dermis substitute, but its application is greatly limited due to ethics and HIV and other virus reasons. At present, according to practice and clinical use, it has been proved that acellular dermal matrix is ​​a new type of wound repair material, and its application field has gradually expanded from the initial treatment of deep burn wounds to plastic surgery, cosmetology, oral cavity, ENT, general surgery and other fields. [0003] ...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/36
CPCA61L27/60A61L27/362A61L27/3687A61L2430/40
Inventor 杨帅周新钦李延浩张建鑫袁召
Owner HENAN HUIBO MEDICAL CO LTD
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