Cotton verticillium wilt antagonistic bacillus, fermentation filtrate and its application

A cotton Verticillium wilt and Bacillus technology, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of serious environmental pollution and the limitations of cultivating resistant varieties, achieve significant antagonism, promote cotton germination, and promote cotton The effect of germination

Active Publication Date: 2019-08-09
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cultivation of resistant varieties has relatively large limitations, and the spraying of chemical pesticides has caused serious environmental pollution.

Method used

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  • Cotton verticillium wilt antagonistic bacillus, fermentation filtrate and its application
  • Cotton verticillium wilt antagonistic bacillus, fermentation filtrate and its application
  • Cotton verticillium wilt antagonistic bacillus, fermentation filtrate and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take 10g of rhizosphere soil from the cotton field in Shihezi, Xinjiang, put it in a 250ml Erlenmeyer flask filled with 90ml of sterile water, oscillate evenly, heat in a water bath at 80°C for 20min to obtain a mixed solution, take 1ml of the mixed solution for gradient dilution, and take 10 -3 、10 -4 、10 -5 Spread 0.1 mL of the dilution gradient dilution on the NA medium (beef extract peptone medium) plate, culture the above NA medium at 30°C for 24 hours in the dark, pick out single colonies with different shapes, sizes, and colors, and streak and purify them. Received on the NA slant medium and stored at 4°C to obtain cotton verticillium dahliat antagonistic bacillus, strain H14.

[0036] After observation, it was found that the single colony of strain H14 on the NA plate was milky white, opaque, round, moist and sticky, with irregular edges and protrusions in the middle; the results of Gram staining showed that strain H14 was a Gram-positive strain, and the bacter...

Embodiment 2

[0040] The cotton Verticillium dahliae pathogenic bacteria preserved on the slant is transferred to the PDA flat plate for activation. After 8 days, a bacterium cake with a diameter of 5 mm is prepared in the edge area of ​​the colony of the Verticillium dahliae dahliae with a sterilized hole puncher, and the bacterial cake is inoculated on a plate with a diameter of 5 mm. In the center of the 90mm PDA plate, culture at 25°C for 4 days, inoculate the bacterial strain H14 in Example 1 at a symmetrical position around the bacterial cake, 20mm away from the bacterial cake, and take the PDA plate not inoculated with the bacterial strain H14 as a control, and place it in a 25°C incubator After dark cultivation for 7 days, the calculated antibacterial rate was 51.23%.

Embodiment 3

[0042]The cotton Verticillium dahliae pathogenic bacteria preserved on the slant is transferred to the PDA flat plate for activation. After 10 days, a bacterium cake with a diameter of 5 mm is prepared in the edge area of ​​the colony of the Verticillium dahliae of cotton with a sterilized hole puncher, and the bacterial cake is inoculated on a plate with a diameter of 5 mm. In the center of the 90mm PDA plate, culture at 25°C for 4 days, inoculate the bacterial strain H14 in Example 1 at a symmetrical position around the bacterial cake, 20mm away from the bacterial cake, and take the PDA plate not inoculated with the bacterial strain H14 as a control, and place it in a 25°C incubator After cultivating in the dark for 10 days, the calculated bacteriostatic rate was 56.25%.

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Abstract

The present invention relates to a cotton Verticillium dahliat antagonistic bacillus, fermentation filtrate and its application. Its preservation number is CGMCC No.12885, and its preservation date is August 22, 2016. It is preserved in the General Microbiology Center of China Microbiological Culture Preservation Management Committee ; The cotton Verticillium wilt antagonistic bacillus is isolated in the cotton rhizosphere soil. The cotton verticillium dahliat antagonistic bacillus of the present invention has significant antagonism to the cotton verticillium dahliae pathogen, and its mycelium growth inhibition rate is 51.23%-56.25%, can promote cotton seedling emergence at the same time, the cotton yellow wilt screened by the present invention The wilt-resistant Bacillus is isolated from the cotton rhizosphere soil. It is safe for the plant itself, the environment, and people. It will not cause drug resistance to humans, and it is not highly toxic. substance. The cotton verticillium dahliat antagonistic bacillus fermentation filtrate of the present invention has an inhibition rate of 78.54%-92.47% on the spore germination of the cotton verticillium dahliae pathogen, and can promote cotton seedling emergence at the same time.

Description

technical field [0001] The invention relates to a bacillus, in particular to a cotton verticillium wilt antagonistic bacillus, fermentation filtrate and application thereof. Background technique [0002] Cotton Verticillium wilt is a soil-borne fungal disease caused by Verticillium dahliae Kleb. It attacks the vascular system of cotton and is called "cotton cancer". In my country's largest commercial cotton production base - Xinjiang, due to continuous cropping, straw returning to the field and the mutation of pathogenic bacteria, the disease is increasing and expanding year by year, which seriously hinders the healthy and sustainable development of Xinjiang's cotton industry. At present, the main methods for controlling cotton Verticillium wilt include cultivating resistant varieties, spraying chemical pesticides and biological control. However, the cultivation of resistant varieties has relatively large limitations, and the spraying of chemical pesticides has caused serio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 李全胜张国丽谢宗铭武冬梅马盼盼王志军高能
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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