Method for preparing rice humidity and temperature sensitive type male sterile material and related gene

Abstract

The invention discloses a method for preparing a rice humidity and temperature sensitive type male sterile material and a related gene. The method for preparing the rice humidity and temperature sensitive type male sterile material comprises the following steps: reducing activity of a protein in a target plant, reducing the content of the protein in the target plant, inhibiting expression of an encoding gene of the protein in the target plant, or knocking off the encoding gene of the protein in the target plant, thereby obtaining a rice humidity and temperature sensitive type male sterile plant, wherein the protein is one of which an amino acid sequence A1) or A2): A1) is sequence 2 in a sequence table; and the amino acid sequence of the sequence 2 in the sequence table is substituted and / or deleted and / or added by one or more amino acid residues and has proteins of same functions. By controlling a glycosyl transferase OsUGT gene and an encoding protein of the gene of rice, the rice humidity and temperature sensitive type male sterile material is obtained, and conversion of rice fertility under different humidity and temperature conditions is achieved.

Description

technical field [0001] The invention relates to a method for preparing rice moisture-sensitive male sterile materials and related genes in the field of biotechnology. Background technique [0002] As the staple food of more than 50% of the world's population, rice has always been concerned about its yield and quality. As an important means to improve rice yield and quality, hybrid rice breeding technology has always been the focus and focus of many scientists. In 1964, Chinese scientist Yuan Longping discovered "natural male sterile plants" in Dongting early indica. Through the research on rice male sterility, he proposed the breeding of rice male sterile lines, male sterile maintainer lines, and male sterile recovery lines. The three-line approach of the line realizes the utilization of rice heterosis. In 1973, Shi Mingsong bred the photosensitive nuclear male sterile material "Nongken 58S" in the late japonica Nongken 58, and proposed the idea of ​​using natural dual-pur...

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Problems solved by technology

[0004] At present, the report on rice moisture-sensitive male sterility is only found in the inventor of the present invention in the Chinese patent No. CN201280006361.6, which discloses that the rice OsOSC8 gene loss-of-function mutant has the characteristics of humidity-sensitive male sterility , the specific molecular mechanism remains to be further studied

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