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Gynostemma total saponin and preparation method thereof

A technology of Gynostemma Gynostemma saponins and Gynostemma pentaphyllum is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., and can solve the problems of high boiling point of n-butanol, large residual amount of final products, and difficult recovery, etc. The effect of short process, low amount of saponins and low cost

Inactive Publication Date: 2017-09-05
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the conventional extraction methods for extracting saponins from Gynostemma pentaphyllum medicinal materials include decoction with water, organic solvent reflux extraction, percolation, etc., as well as various new technologies such as microwave extraction, ultrasonic extraction, and enzyme-assisted extraction; but There are not many refining methods, mainly using the characteristics of high solubility of saponins in n-butanol, and extracting with n-butanol, but n-butanol has a high boiling point, recovery is not easy, and the residual amount in the final product is relatively large. Industrial production is not easy

Method used

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  • Gynostemma total saponin and preparation method thereof

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Effect test

Embodiment 1

[0024] Take 1,500 grams of Gynostemma pentaphylla, cut into short pieces, add 20 times the amount of 70% ethanol, reflux for extraction for 3 hours, filter, add ethanol to the filtrate to the end, add 7,500 mL of water, boil for 30 minutes, filter, and put the filtrate on AB -8 column (treated, Vol=1500mL), after 5 hours of sample loading, discard the effluent; wash 2BV with water, discard the washing solution; then wash 2BV (3000mL) with 0.2% sodium hydroxide, discard the alkaline washing solution (to remove flavonoids), 1BV / h; then wash with water until neutral (about 6BV), discard the washing solution, 2BV / h; then elute with 40% ethanol 4BV (6000mL), 1BV / h, 40% Discard the ethanol lotion; then elute with 80% ethanol 4BV (6000mL), 1BV / h, collect the 80% ethanol lotion, recover the ethanol, concentrate into a thick paste, and then dry under reduced pressure to obtain the total saponins of Gynostemma pentaphyllum; The total saponin content was determined by spectrophotometry, ...

Embodiment 2

[0026] Take 3000 grams of Gynostemma pentaphyllum, crush it into coarse powder, add 15 times the amount of 85% methanol, reflux for extraction for 2 hours, filter, add methanol to the filtrate to the end, add 20L of water, boil for 30 minutes, filter, and put the filtrate on D101 Column (processed, Vol=3000mL), sample loading was completed in 6 hours, and the effluent was discarded; 3BV was washed with water, and the water wash was discarded; then 2BV (6000mL) was washed with 0.5% potassium hydroxide, and the alkali wash was discarded, 1.5 BV / h; then wash with water until neutral (about 6BV), discard the water washing solution, 2BV / h; then elute with 30% methanol 4BV (6000mL), 1BV / h, discard the 30% methanol washing solution; 70% methanol 5BV elution, 1BV / h, collect 70% methanol lotion, reclaim methanol, concentrate into a thick paste, and then dry under reduced pressure, which is the total saponins of Gypenoside; In terms of A, the content is 94%.

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Abstract

The invention relates to gynostemma total saponin and a preparation method thereof, belongs to the field of traditional Chinese medicine and relates to a Chinese medicine active part and a extracting, purifying and refining method thereof. Gynostemma herbs are placed in a reflux extractor to be subjected to reflux extraction with high-concentration ethanol prior to filtration and alcohol removal to obtain a non-alcohol filtrate, the filtrate is absorbed and eluted with macroporous resin, eluants refer to water, low-concentration alkali, low-concentration alcohol and high-concentration alcohol, the eluants are obtained, and the high-concentration alcohol eluant is retained, concentrated and evaporated to dryness to obtain the gynostemma total saponin. For the prepared gynostemma total saponin, total saponin content measured by gypenosides A with the ultraviolet spectroscopy is 92-94%. The method is high in efficiency, simplified in steps, short in cycle, low in cost and suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and relates to effective parts of traditional Chinese medicines and methods for extracting, purifying and refining them, in particular to a method for extracting and refining gypenosides from gynostemma pentaphylla whole herb raw materials and a preparation method thereof. Background technique [0002] The prior art discloses that Gynostemma pentaphyllum is a perennial climbing herb of Cucurbitaceae Gynostemma pentaphyllum, also known as Aescin, five-leaved ginseng, public Luoguodi, ground root and Aescin, etc., and its Latin name is Gynostemmapentaphyllum (Thunb.) Makino. The plant is divided into 13 species, which are distributed in my country, Japan, North Korea and Southeast Asian countries. Among them, according to the survey, there are 11 species in our country, namely: Gynostemma unileaf, Gynostemma light-leaf, Gynostemma burma, Gynostemma wool, and Gynostemma long-stemmed , Gyn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/424
CPCA61K36/424A61K2236/15A61K2236/333A61K2236/39A61K2236/53A61K2236/55
Inventor 胡义扬冯琴彭景华田华捷
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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