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A method for extracting phytol from marine coccidioides

A technology of marine coccus algae and phytol, which can be used in the separation/purification of hydroxyl compounds, organic chemistry, antitumor drugs, etc., and can solve problems such as less research.

Inactive Publication Date: 2020-11-06
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the active substances of marine coccidioides lipids

Method used

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  • A method for extracting phytol from marine coccidioides
  • A method for extracting phytol from marine coccidioides

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Embodiment 1

[0023] Embodiment 1 A method of extracting phytol from marine coccidioides

[0024] The invention discloses a method for extracting phytol from marine coccidioliths, which comprises the following steps: extraction with ethyl acetate; primary chromatography with a reversed-phase silica gel column; secondary chromatography with a reversed-phase silica gel column; Sephadex LH-20 was used for chromatography; normal phase silica gel column was used for chromatography; phytol was extracted from marine coccidioides by PTLC preparation method.

[0025] As a preferred mode of embodiment, the above-mentioned method for extracting phytol specifically includes the following steps:

[0026] Step 1. Preparation of the ethyl acetate phase extract of marine coccidioides: first leaching with ethanol solution to obtain ethanol extract, then extracting with ethyl acetate as the extraction agent, combining the extracts, concentrating under reduced pressure to obtain marine coccolithophores Algae...

Embodiment 2

[0038] Example 2 Cytotoxic activity experiment

[0039] a. Cell cryopreservation:

[0040] Prepare 10% DMSO calf serum cryopreservation solution; take LO2 and Hep G2 cells grown in logarithmic phase, digest monolayer growing cells with trypsin; centrifuge at 1000rpm for 5min; remove supernatant, add new cryopreservation solution; aliquot Put the name and time in the cryopreservation tube; put the cryopreservation tube containing the cells in a -20°C refrigerator for 2 hours, then put it in a -70°C refrigerator overnight, quickly take out the cryopreservation tube, and transfer it to liquid nitrogen for cryopreservation .

[0041] b. Cell recovery:

[0042] Take out the cell cryopreservation tube from liquid nitrogen, thaw it quickly in a 37°C water bath, and control this process within 1 minute; suck out the cell suspension into the centrifuge tube and add 10 times the medium, mix gently; centrifuge at 1000rpm for 5min; Pour off the supernatant, wash again, count on a hemoc...

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Abstract

The invention discloses a method for extracting phytol from coccolithophores. The method comprises the steps of using ethyl acetate to conduct extraction; using an antiphase silica gel column to conduct primary chromatography; using the antiphase silica gel column to conduct secondary chromatography; using a sephadex column LH-20 to conduct chromatography; using a normal phase silica gel column to conduct chromatography; extracting phytol from coccolithophores through a PTLC preparing method. According to the method for extracting phytol from coccolithophores, phytol of coccolithophores ethyl acetate phase extracts is separated and purified for the first time, and research is made on cytotoxicity of phytol, so that a basis is laid for subsequent large-scale production of phytol.

Description

technical field [0001] The invention relates to the technical field of biological preparations, in particular to a method for extracting phytol from marine coccidioides. Background technique [0002] Coccolithophores are a class of unicellular marine micro-phytoplankton belonging to the class Prymnesiophyceae (Haptophyta=Prymnesiophyta). The metabolites of marine coccidioides are rich in a variety of biologically active substances, such as docosahexaenoic acid (DHA), polyketides, etc., especially the total lipid content in marine microalgae cells can reach the dry weight of cells 50%, these compounds have good antibacterial, anti-oxidation, anti-tumor and other biological functions, and have broad application prospects in the field of biotechnology. However, there are few studies on the active substances of marine coccidioides lipids. [0003] In view of this, the inventor has researched and designed a method for extracting phytol from marine coccidioides, and this case ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C29/74C07C29/76C07C29/78C07C33/03A61P35/00
CPCC07C29/74C07C29/76C07C29/78C07C33/03
Inventor 刘静雯马亚瑞李健李桂玲
Owner JIMEI UNIV
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