Hollow Prussian blue-based thermal stimuli-responsive drug release nano-carrier and preparation method thereof
A technology of Prussian blue and nano-carriers, applied in the field of nano-materials, can solve problems such as drug selection limitations, and achieve the effects of being beneficial to clinical use, improving utilization, and reducing harm
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Embodiment 1
[0040] This embodiment prepares blank nanocarriers as follows:
[0041] (1) First prepare hollow Prussian blue nanoparticles by hydrothermal method:
[0042] Weigh 3g of polyvinylpyrrolidone (PVP) and dissolve it in 40mL of 0.01mol / L hydrochloric acid solution, stir until completely dissolved, then add 132mg of potassium ferricyanide, stir until completely dissolved, and react at 80°C for 20 hours. get mesoporous Prussian blue;
[0043] Dissolve 20 mg of mesoporous Prussian blue in 20 mL of 0.1 mol / L hydrochloric acid solution, then add 200 mg of polyvinylpyrrolidone, stir for 1 hour, and react at 140°C for 2 hours to obtain hollow Prussian blue nanoparticles;
[0044] (2) Dissolve 300 mg of myristyl alcohol in 6 mL of methanol solution, stir at 50° C. until uniformly mixed to obtain a tetradecyl alcohol solution; disperse 15 mg of hollow Prussian blue nanoparticles into 3 mL of methanol solution to obtain a nanoparticle suspension;
[0045] (3) After mixing the myristyl alcoh...
Embodiment 2
[0049] This embodiment prepares nanocarriers loaded with hydrophilic drugs as follows:
[0050] (1) Prepare hollow Prussian blue nanoparticles by the same method as in Example 1.
[0051] (2) Dissolve 300mg of myristyl alcohol in 6mL, 1mg / mL methanol solution of the hydrophilic drug doxorubicin, stir at 50°C until uniformly mixed to obtain a mixed solution; disperse 15mg of hollow Prussian blue nanoparticles into 3mL of methanol solution In, obtain nanoparticle resuspension;
[0052] (3) After mixing the mixed solution and the nanoparticle resuspension, stir for 1 h, so that part of the methanol solution dissolved with tetradecyl alcohol and doxorubicin enters the hollow of the hollow Prussian blue nanoparticle; Stirring for 4 hours to completely volatilize the methanol solution inside and outside the hollow Prussian blue nanoparticles to obtain a drug-loaded nanocarrier dispersion dispersed in liquid myristyl alcohol;
[0053] (4) Continue constant temperature, and add deio...
Embodiment 3
[0061] In this example, a drug-loaded nanocarrier was prepared according to the same method as in Example 2, except that the hydrophilic drug doxorubicin in Example 2 was replaced with the hydrophobic drug camptothecin.
[0062] image 3 B is the ultraviolet absorption spectrum comparison chart of the nanocarrier carrying the hydrophobic drug camptothecin in this embodiment and the blank nanocarrier in Example 1 and free camptothecin, as can be seen from the figure, in the range of 350-400nm, hi There is an absorption peak for dendriticine, and within this range, the absorption of drug-loaded nanocarriers increases accordingly, which indicates that the nanocarriers are successfully loaded with drugs.
[0063] Image 6 b is the drug release curve of the nanocarrier loaded with the hydrophobic drug camptothecin prepared in this example at different temperatures. 1mL was placed in a 5000Da dialysis bag, and then the dialysis bag was placed in 30mL of 0.1vt% Tween-80 aqueous sol...
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