Method of preparing fish meal substitute proteins by using aquatic protein and feather powder through fermentation

A fish meal replacement and feather meal technology, which is applied in application, climate change adaptation, animal feed, etc., can solve the problems of difficult large-scale application of feather meal, high input cost, and difficult large-scale production, etc., and achieve easy large-scale production. Large-scale production, high cost, low cost effect

Pending Publication Date: 2017-09-19
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing methods of biological treatment of feather meal are difficult to obtain large-scale application in my country's feed industry
Although the method of liquid fermentation is easy to produce on a large scale, it needs to purchase fermentation tanks and other related equipmen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028]Example 1: Insert the thermostable facultative anaerobic bacteria Bacillusmethylotrophicus HL-4 that can produce keratinase preserved on a slant into the LB liquid medium cooled after sterilization, and cultivate it at 55°C and 150r / min for 12h to prepare Bacillus methylotrophicus HL-4 seed solution is ready for use; mix bran and water in a mass ratio of 1:1, and mix well to prepare a bran medium. Bran culture is sterilized at 121°C for 30 minutes before use; the prepared Bacillus methylotrophicus HL-4 seed solution was added to the bran culture medium cooled after sterilization and mixed evenly, and then the bran culture medium added to the strain was divided into sterilized fermentation pans, and the thickness of the filling was 4cm, the surface of the fermentation medium in the shallow dish is covered with sterilized wet gauze, and then the shallow dish is placed on the shelf in the sterilized solid fermentation room for fermentation. The ambient temperature is control...

Embodiment 2

[0029] Example 2: Insert the thermostable facultative anaerobic bacterium Bacillusmethylotrophicus HL-4 that can produce keratinase preserved on the slant into the LB liquid medium cooled after sterilization, and cultivate it at 55°C and 150r / min for 12h to prepare Bacillus methylotrophicus HL-4 seed solution is ready for use; mix bran and water in a mass ratio of 1:1, and mix well to prepare a bran medium. Bran culture is sterilized at 121°C for 30 minutes before use; the prepared Bacillus methylotrophicus HL-4 seed solution was added to the bran culture medium cooled after sterilization and mixed evenly, and then the bran culture medium added to the strain was divided into sterilized fermentation pans, and the thickness of the filling was 4cm, the surface of the fermentation medium in the shallow dish is covered with sterilized wet gauze, and then the shallow dish is placed on the shelf in the sterilized solid fermentation room for fermentation. The ambient temperature is con...

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Abstract

The present invention discloses a method of preparing fish meal substitute proteins by using an aquatic protein and feather powder through fermentation. The method comprises the following main steps: high temperature resistant facultative anaerobic bacteria of bacillus methylotrophicus HL-4 capable of producing keratinase are cultured to obtain a microorganism agent for a standby application; minced and crushed aquatic proteins and hydrolyzed feather powder are mixed, nutrients and water are added, and the materials are mixed evenly to obtain a fermentation medium; the bacillus methylotrophicus microorganism agent and protease are added into the fermentation medium, the materials are mixed evenly, and the mixture is stacked to be subjected to fermentation, wherein the fermentation is conducted in a natural standing manner for a fermentation time of 48 h-72 h; and after the fermentation, the product is dried, the dried product is crushed, and the crushed product is packaged to obtain a finished product of the fish meal substitute proteins. The prepared fish meal substitute proteins can be used to greatly replace imported high quality fish meal in aquatic feeds, obviously reduce feed costs and have good application prospects and practical significances.

Description

technical field [0001] The invention relates to a method for preparing fishmeal substitute protein by fermenting aquatic protein and feather meal, and belongs to the technical field of aquatic product processing and utilization. Background technique [0002] Fishmeal has always been an indispensable high-quality protein source in aquafeeds because of its good palatability and rich nutrition, especially the balanced amino acid composition and rich content of unknown growth factors. The amount of fish meal in aquatic feed usually accounts for 30% to 60% of the formula, which is the largest component of its cost. With the continuous expansion of aquatic animal breeding worldwide, the global demand for fishmeal continues to grow. Currently, the global annual fishmeal supply is about 7 million tons. However, due to various reasons such as adverse climate effects, overfishing, pollution of the marine environment, and destruction of the ecological environment, fishery resources h...

Claims

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Application Information

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IPC IPC(8): A23K10/26A23K10/22A23K10/12A23K10/14A23K10/30A23K20/26A23K20/20A23K50/80
CPCY02A40/818
Inventor 刘唤明洪鹏志周春霞杨萍刘艳芬邓楚津
Owner GUANGDONG OCEAN UNIVERSITY
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