A method for extracting and displaying blood latent fingerprints based on western blotting

A technology of protein imprinting and latent fingerprinting, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of difficult inspection and identification, difficult discovery and extraction of blood fingerprints, and achieve high detection reliability and avoid non-specific adsorption , the effect of high sensitivity

Active Publication Date: 2020-06-12
CHINA PHARM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In order to make up for the deficiencies of the existing technology and solve the problems in the prior art that the discovery and extraction of blood fingerprints are difficult and the later inspection and identification are difficult, the present invention provides a method for extracting and displaying blood latent fingerprints based on protein imprinting

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  • A method for extracting and displaying blood latent fingerprints based on western blotting
  • A method for extracting and displaying blood latent fingerprints based on western blotting

Examples

Experimental program
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Effect test

Embodiment 1

[0042] The method for extracting and displaying blood latent fingerprints based on the western blot method includes:

[0043] 1) Use a spring blood collection needle to collect a drop of blood on the fingertip, apply it to the finger, and dry it;

[0044] 2) Press the fingerprint on the non-porous glass substrate; the glass substrate is ultrasonically cleaned with absolute ethanol and water for 20 minutes;

[0045] 3) transfer the fingerprint sample on the substrate to 8% SDS-PAGE glue;

[0046] The 8% SDS-PAGE gel was prepared as follows:

[0047] Water: 4.6ml; 30% acrylamide: 2.7ml; 1.5M Tris (PH 8.8): 2.5ml; 10% SDS: 0.1ml; TEMED: 6μl.

[0048] 4) Use a wet transfer instrument, set the voltage to 120V, and transfer the time to 30 minutes, and transfer the target protein of the fingerprint sample on the SDS-PAGE gel to the PVDF membrane;

[0049] 5) After the transfer is completed, immerse the PVDF membrane in 5% (w / v) skim milk to seal for 1 hour;

[0050] 6) Wash the e...

Embodiment 2

[0060] The method for extracting and displaying blood latent fingerprints based on the western blot method includes:

[0061] 1) Use a spring blood collection needle to collect a drop of blood on the fingertip, apply it to the finger, and dry it;

[0062] 2) Press the fingerprint on the porous paper substrate; the paper substrate is ordinary A4 paper;

[0063] 3) transfer the fingerprint sample on the substrate to 10% SDS-PAGE glue;

[0064] A 10% SDS-PAGE gel was prepared as follows:

[0065] Water: 4.1 ml; 30% acrylamide: 3.3 ml; 1.5M Tris (PH 8.8): 2.5 ml; 10% SDS: 0.1 ml; TEMED: 6 μl.

[0066] 4) Use a wet transfer instrument, set the voltage to 110V, and transfer the time to 30 minutes, and transfer the target protein of the fingerprint sample on the SDS-PAGE gel to the PVDF membrane;

[0067] 5) After the transfer is completed, immerse the PVDF membrane in 4% (w / v) skim milk to seal for 1.5h;

[0068] 6) Wash the excess milk on the membrane with TBST, wash 3 times, 5...

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Abstract

The invention discloses a method for extracting and showing bloody fingerprint on the basis of protein blotting, and belongs to the field of fingerprint detection. The method comprises the following steps: transferring bloody fingerprint samples remaining on different objects to SDS-PAGE glue; transferring target protein to a PVDF film in a wet transfer mode; selecting a primary antibody capable of specifically recognizing the target protein and a secondary antibody labeled by horse radish peroxidase, and connecting the horse radish peroxidase to a target detected object by specific reaction of the antibodies and the target protein; and then showing the bloody fingerprint by chemiluminiscence. The protein ingredient in the bloody fingerprint can be extracted accurately, and meanwhile, the complete morphology of the fingerprint can be retained, namely, an ingredient recognition function and a fingerprint showing function are fulfilled. The method has the characteristics of simplicity, high efficiency and easiness in operation, and has good application prospect on recognition of bloody fingerprints remaining on criminal case crime scenes.

Description

[0001] 1. Technical field [0002] The invention relates to the field of fingerprint detection, in particular to a method for extracting and displaying blood latent fingerprints based on a protein imprinting method. [0003] 2. Background technology [0004] Fingerprint identification is one of the most reliable methods for personal identification. At present, fingerprint identification accounts for about 70% of the cases detected by the police using criminal technology. Fingerprints were once recognized by the judiciary as "the first physical evidence". However, with the development of intelligence in criminal activities, the discovery and extraction rate of clear and complete fingerprints has dropped significantly. In particular, there is a high probability that the suspect's blood fingerprints will be left on the murder scene, but the discovery and extraction of blood fingerprints are difficult, and the later inspection and identification are difficult. [0005] The Chines...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/535
CPCG01N33/535G01N33/68
Inventor 刘鄂湖许林茹吕志敏
Owner CHINA PHARM UNIV
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