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An adsorbent for removing low-density lipoprotein from blood and its preparation method

A low-density lipoprotein and adsorbent technology, applied in blood circulation treatment, chemical instruments and methods, suction devices, etc., can solve the problems of complicated operation, rare antibodies, and high price, and achieve good biocompatibility and cheap raw materials And easy to obtain, the effect of preparation method is simple

Active Publication Date: 2020-04-17
佛山市博新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although immunosorbents have high adsorption selectivity and good adsorption effect, the antibodies used are rare, expensive and difficult to store. Most of these products are treated by plasma adsorption. Although they can be reused, the operation is complicated and the consumables are expensive and expensive. Does not meet the needs of my country's national conditions
Although many scholars have devoted themselves to the study of lipid adsorbents in recent years, the results have not been well applied in practice.

Method used

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  • An adsorbent for removing low-density lipoprotein from blood and its preparation method
  • An adsorbent for removing low-density lipoprotein from blood and its preparation method
  • An adsorbent for removing low-density lipoprotein from blood and its preparation method

Examples

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preparation example Construction

[0020] A method for preparing an adsorbent for removing low-density lipoprotein from blood includes the following steps:

[0021] (1) Diepoxy activation: Place 10-30mL of solid porous cellulose microspheres in a 50mL or 100mL round-bottomed flask, and add 10-30mL dimethyl sulfoxide (DMSO), 5-10mL 1.0mol / L NaOH solution, 5-20mL 1,4-butanediol diglycidyl ether (BDE), stirred at 20-40℃ for 2-8h. After the reaction is completed, take out suction filtration, first wash with 50% acetone solution until no BDE remains, and finally wash with distilled water until no acetone remains.

[0022] (2) Coupling of hydrophobic ligands: Place the porous microspheres prepared in step (1) in a 50mL or 100mL round-bottom flask, add 5-15mL DMSO, 5-15mL 0.5mol / L NaOH solution and 1 ~10g α-tocopherol, stirred at 20~50℃ and protected from light for 4~24h. After the reaction is completed, first wash the unreacted α-tocopherol with a 50% ethanol solution, and then wash it with distilled water.

[0023] (3) ...

Embodiment 1

[0027] Place 10mL of the settled porous cellulose microspheres in a 50mL Erlenmeyer flask, add 10mL of DMSO, 10mL of 1.0mol / L NaOH solution, and 5mL of 1,4-butanediol diglycidyl ether ( BDE), react at 25°C for 4h. After the reaction is completed, take out suction filtration, first wash with 50% acetone solution until no BDE remains, and finally wash with distilled water until no acetone remains.

[0028] The activated porous microspheres were placed in a 50 mL Erlenmeyer flask, and 5 mL of DMSO, 5 mL of 0.5 mol / L NaOH solution and 2 g of α-tocopherol were added, and the reaction was stirred for 12 hours at 30°C and protected from light. After the reaction is complete, first wash the unreacted α-tocopherol with 50% ethanol solution, then wash it with distilled water, transfer it to an Erlenmeyer flask, and add 5 mL of dimethyl sulfoxide (DMSO) and 5 mL of 2.0 mol / L NaOH solution and 2 mL of epichlorohydrin were stirred and reacted at 40° C. for 3 h. After the reaction is comple...

Embodiment 2

[0031] Put 5mL of the settled porous cellulose microspheres in a 50mL Erlenmeyer flask, add 5mL DMSO, 2mL 1.0mol / L NaOH solution, 1mL 1,4-butanediol diglycidyl ether ( BDE), react at 30°C for 6h. After the reaction is completed, take out suction filtration, first wash with 50% acetone solution until no BDE remains, and finally wash with distilled water until no acetone remains.

[0032] Place the activated porous microspheres in a 50 mL Erlenmeyer flask, add 5 mL of DMSO, 3 mL of 0.5 mol / L NaOH solution and 1 g of α-tocopherol, and stir for 15 hours at 40° C. and avoid light. After the reaction is complete, first wash the unreacted α-tocopherol with 50% ethanol solution, then wash it with distilled water, transfer it to an Erlenmeyer flask, and add 5 mL of dimethyl sulfoxide (DMSO) and 5 mL of 2.0 mol / L NaOH solution and 1 mL of epichlorohydrin were stirred and reacted at 40° C. for 2.5 h. After the reaction is completed, take out suction filtration, first wash with 50% aceton...

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Abstract

The invention discloses an adsorbent for eliminating low-density lipoprotein in blood and a preparation method of the adsorbent. Hydrophobic aglucon and polyanion aglucon are coupled with an adsorption carrier, and the mass ratio of the hydrophobic aglucon to the polyanion aglucon ranges from (1:5) to (5:1). By coupling the hydrophobic aglucon and the polyanion aglucon with the adsorption carrier simultaneously, and by virtue of high hydrophobic affinity of the hydrophobic aglucon with lipoprotein, and an electrostatic combination function of polyanion with the lipoprotein, specific adsorption is implemented through synergism of the hydrophobic aglucon and the polyanion with the lipoprotein, and thus the purpose of specifically eliminating TG (Triglyceride) and LDL (Low Density Lipoprotein) in blood is achieved. The adsorbent is a double-hydrophilic adsorbent which is high in adsorption capacity, good in biocompatibility and applicable to treatment on hyperlipidaemia through whole blood perfusion.

Description

Technical field [0001] The invention relates to the field of biomedicine, in particular to a preparation method of an absorbent for removing blood low-density lipoprotein. Background technique [0002] The number of people who die from cardiovascular and cerebrovascular diseases in my country is as high as 3 million each year, accounting for 51% of the total causes of death, and has become the number one killer of human deaths. Epidemiological studies have shown that atherosclerosis is the main pathogenic factor of cardiovascular disease, and the high concentration of low-density lipoprotein (LDL) in the blood is the main predisposing factor of atherosclerosis. An increase in the concentration of LDL in the blood will cause an increase in blood viscosity, resulting in a decrease in blood flow rate. In the damaged or narrowed blood vessel, oxidized low-density lipoprotein (ox-LDL) easily forms plaques with other proteins. These plaques Gradually accumulate and cause atheroscleros...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30A61M1/36
CPCA61M1/3687B01J20/26B01J20/28011
Inventor 姜建明梁达星
Owner 佛山市博新生物科技有限公司
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