Deer blood protein polypeptide and application

Abstract

The invention discloses a deer blood protein polypeptide. A preparation method thereof comprises the following steps: separation of blood corpuscles, ultrasonically assisting hemolysis, microwave-assisting alkali enzyme enzymolysis, microwave-assisting papain enzymolysis and concentrating. The molecule has higher uniformity, is high in oligopeptides proportion and is easily absorbed; the deer blood protein polypeptide has higher fatigue resistance and antioxidant activity and better effect; the deer blood protein polypeptide used for preparing healthcare wine has higher stability, is difficult to form precipitation or turbidity and has wide application prospect in the field of healthcare products.

Description

technical field

[0001] The invention relates to the technical field of health products, in particular to a deer blood protein polypeptide and its application. Background technique

[0002] Deer blood refers to the antler blood and blood of deer animals such as sika deer and red deer. Studies have shown that deer blood has the functions of enhancing immunity, replenishing blood, delaying aging, anti-fatigue, improving sexual function, benefiting essence, calming the nerves, promoting metabolism and wound healing and other functions. Over the years, the use of deer blood resources in my country is limited to the utilization and processing of raw blood, most of which exist in the form of deer blood wine. In some developed countries, the use of animal blood enzymolysis has begun to develop peptide reagents and drugs. Therefore, the development of deer blood protein products is of great significance to the development of deer blood resources.

[0003] The chemical composition ...

Examples

preparation example Construction

[0030] The present invention provides a deer blood protein polypeptide, the preparation method of which comprises sequentially:

[0031] Separation of blood cells: add anticoagulant to fresh deer blood, disperse to obtain anticoagulant blood sample, centrifuge the anticoagulant blood sample, discard plasma, and collect blood cells;

[0032] In this step, the anticoagulant is preferably a mixed solution of citric acid, sodium citrate and glucose, wherein the weight ratio of citric acid, sodium citrate and glucose is (0.8-1):(0.3-0.6 ): 1, that is, the mixed anticoagulant can make up for the defects such as the unstable pH value of the system caused by the independent anticoagulant;

[0033] Ultrasound-assisted hemolysis: Wash the blood cells with normal saline, add 1-2 times the volume of distilled water, adjust the pH to 9-10 with dilute NaOH, use 35-37°C ultrasonic-assisted hemolysis, centrifuge, and collect the supernatant;

[0034] Ultrasonic assistance is used to effectiv...

Embodiment 1

[0041] A deer blood protein polypeptide, the preparation method of which comprises sequentially:

[0042] Separation of blood cells: Add 8g of anticoagulant to 1L of fresh deer blood, disperse to obtain an anticoagulant blood sample, centrifuge the anticoagulant blood sample at 3300rpm for 12min, discard the plasma, and collect blood cells; in this step, the anticoagulant is citric acid, lemon Sodium acid and glucose are mixed in a weight ratio of 1:(0.3-0.6):1;

[0043] Ultrasound-assisted hemolysis: wash the blood cells with normal saline, add an equal volume of distilled water, adjust the pH to 9-10 with 0.5 mol / L NaOH, use ultrasonic-assisted hemolysis at 35-37°C for 15 minutes, ultrasonic power 350W, and centrifuge at 3300rpm for 12 minutes. Collect the supernatant;

[0044] Microwave-assisted alkaline enzyme enzymolysis: add distilled water to the supernatant to dilute to 9g blood cells / L, add alkaline protease to make the enzyme concentration 6500U / g blood cells, micro...

Embodiment 2

[0055] A deer blood protein polypeptide, the preparation method of which comprises sequentially:

[0056] Separation of blood cells: add 8g of anticoagulant to 1L of fresh deer blood, disperse to obtain an anticoagulant blood sample, centrifuge the anticoagulant blood sample at 3300rpm for 12min, discard the plasma, and collect blood cells; in this step, the anticoagulant is citric acid, lemon Sodium acid and glucose are mixed in a weight ratio of 1:0.5:1;

[0057] Ultrasound-assisted hemolysis: wash the blood cells with normal saline, add an equal volume of distilled water, adjust the pH to 9-10 with 0.5mol / L NaOH, use ultrasonic-assisted hemolysis at 35-37°C for 15min, ultrasonic power 400W, and centrifuge at 3500rpm for 10min. Collect the supernatant;

[0058] Microwave-assisted alkaline enzyme enzymatic hydrolysis: add distilled water to the supernatant to dilute to 8g blood cells / L, add alkaline protease to make the enzyme concentration 7000U / g blood cells, microwave-ass...

technical field

[0001] The invention relates to the technical field of health products, in particular to a deer blood protein polypeptide and its application. Background technique

[0002] Deer blood refers to the antler blood and blood of deer animals such as sika deer and red deer. Studies have shown that deer blood has the functions of enhancing immunity, replenishing blood, delaying aging, anti-fatigue, improving sexual function, benefiting essence, calming the nerves, promoting metabolism and wound healing and other functions. Over the years, the use of deer blood resources in my country is limited to the utilization and processing of raw blood, most of which exist in the form of deer blood wine. In some developed countries, the use of animal blood enzymolysis has begun to develop peptide reagents and drugs. Therefore, the development of deer blood protein products is of great significance to the development of deer blood resources.

[0003] The chemical composition ...

Examples

preparation example Construction

[0030] The present invention provides a deer blood protein polypeptide, the preparation method of which comprises sequentially:

[0031] Separation of blood cells: add anticoagulant to fresh deer blood, disperse to obtain anticoagulant blood sample, centrifuge the anticoagulant blood sample, discard plasma, and collect blood cells;

[0032] In this step, the anticoagulant is preferably a mixed solution of citric acid, sodium citrate and glucose, wherein the weight ratio of citric acid, sodium citrate and glucose is (0.8-1):(0.3-0.6 ): 1, that is, the mixed anticoagulant can make up for the defects such as the unstable pH value of the system caused by the independent anticoagulant;

[0033] Ultrasound-assisted hemolysis: Wash the blood cells with normal saline, add 1-2 times the volume of distilled water, adjust the pH to 9-10 with dilute NaOH, use 35-37°C ultrasonic-assisted hemolysis, centrifuge, and collect the supernatant;

[0034] Ultrasonic assistance is used to effectiv...

Embodiment 1

[0041] A deer blood protein polypeptide, the preparation method of which comprises sequentially:

[0042] Separation of blood cells: Add 8g of anticoagulant to 1L of fresh deer blood, disperse to obtain an anticoagulant blood sample, centrifuge the anticoagulant blood sample at 3300rpm for 12min, discard the plasma, and collect blood cells; in this step, the anticoagulant is citric acid, lemon Sodium acid and glucose are mixed in a weight ratio of 1:(0.3-0.6):1;

[0043] Ultrasound-assisted hemolysis: wash the blood cells with normal saline, add an equal volume of distilled water, adjust the pH to 9-10 with 0.5 mol / L NaOH, use ultrasonic-assisted hemolysis at 35-37°C for 15 minutes, ultrasonic power 350W, and centrifuge at 3300rpm for 12 minutes. Collect the supernatant;

[0044] Microwave-assisted alkaline enzyme enzymolysis: add distilled water to the supernatant to dilute to 9g blood cells / L, add alkaline protease to make the enzyme concentration 6500U / g blood cells, micro...

Embodiment 2

[0055] A deer blood protein polypeptide, the preparation method of which comprises sequentially:

[0056] Separation of blood cells: add 8g of anticoagulant to 1L of fresh deer blood, disperse to obtain an anticoagulant blood sample, centrifuge the anticoagulant blood sample at 3300rpm for 12min, discard the plasma, and collect blood cells; in this step, the anticoagulant is citric acid, lemon Sodium acid and glucose are mixed in a weight ratio of 1:0.5:1;

[0057] Ultrasound-assisted hemolysis: wash the blood cells with normal saline, add an equal volume of distilled water, adjust the pH to 9-10 with 0.5mol / L NaOH, use ultrasonic-assisted hemolysis at 35-37°C for 15min, ultrasonic power 400W, and centrifuge at 3500rpm for 10min. Collect the supernatant;

[0058] Microwave-assisted alkaline enzyme enzymatic hydrolysis: add distilled water to the supernatant to dilute to 8g blood cells / L, add alkaline protease to make the enzyme concentration 7000U / g blood cells, microwave-ass...

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