A polypeptide with memory-improving effect and its separation and preparation method and application
A technology for improving memory and inhibiting activity, which can be used in the preparation methods of peptides, medical preparations containing active ingredients, biochemical equipment and methods, etc. Powerful, inexpensive, high-purity effects
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[0043] The separation and preparation method of the polypeptide of the present invention comprises the following steps:
[0044](1) Remove the head and viscera of anchovies, clean them, and put them into a blender to make minced meat; add water twice the weight of the minced meat, and add protease at 0.5% of the mass of the minced meat (based on the quality of the minced fish, the amount of papain is The addition amount accounts for 0.25%, the addition amount of Alcalase 2.4L accounts for 0.25%), incubating enzymolysis at 45°C for 6h, then heating at 95°C for 15min to inactivate the enzyme, cooling to room temperature, centrifuging at 3500r / min for 10min, Collecting the supernatant to obtain an anchovy protein enzymatic hydrolyzate;
[0045] (2) Separation and purification of anchovy protein enzymatic hydrolyzate with Sephadex G-25 gel column, elution with deionized water at a flow rate of 0.5mL / min, detection wavelength is 220nm, a total of 6 components were collected and elu...
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