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Fluorescent nanoparticles based on imine linkers and their application in the detection of hg2+ and acetylcholinesterase

A fluorescent nanoparticle and acetylcholinesterase technology, applied in nanotechnology, nanotechnology, nano-optics, etc., can solve the problems of easy false positive or false negative results, cumbersome sample preparation procedures, large sample background interference, etc., and achieve uniformity Spherical particle size distribution, good thermal stability and high accuracy

Inactive Publication Date: 2018-07-10
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current methods for detecting acetylcholinesterase (such as Ellman method, fluorescence analysis method, electrochemical method and diffraction method, etc.) still have deficiencies, such as prone to false positive or false negative results, low detection sensitivity, lack of specific substrate, sample Large background interference, complicated sample preparation procedures, long measurement time, etc.

Method used

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  • Fluorescent nanoparticles based on imine linkers and their application in the detection of hg2+ and acetylcholinesterase
  • Fluorescent nanoparticles based on imine linkers and their application in the detection of hg2+ and acetylcholinesterase
  • Fluorescent nanoparticles based on imine linkers and their application in the detection of hg2+ and acetylcholinesterase

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Weigh 48.64g (0.30mmol) of 1,3,5-benzenetricarbaldehyde and 68.47g (0.45mmol) of 3,5-diaminobenzoic acid, dissolve them in 20mL of absolute ethanol, and then dissolve 1,3,5- The ethanol solution of benzenetricarbaldehyde was quickly poured into the ethanol solution of 3,5-diaminobenzoic acid, stirred at room temperature for 30min, then centrifuged at 10000rpm for 5min, washed 3 times with absolute ethanol, and then dissolved in 20mL N,N-di Reflux in methylformamide at room temperature for 4 hours, centrifuge to remove N,N-dimethylformamide, then reflux with absolute ethanol for 2 hours, centrifuge, wash, and vacuum-dry at room temperature for 12 hours to obtain fluorescent nanoparticles based on imine linkages.

[0031] The inventors used PE LS55 fluorescence spectrophotometer, infrared spectrometer, transmission electron microscope, powder X-ray diffractometer, physical adsorption instrument, and thermogravimetric analyzer to characterize the obtained fluorescent nanopa...

Embodiment 2

[0033] In Example 1, the fluorescent nanoparticles based on imine connection are used in the detection of Hg 2+ The application in the specific method is as follows:

[0034] 1. Disperse 0.0034 mg of imine-based fluorescent nanoparticles in 1 mL of ultrapure water to prepare a 3.4 μg / mL fluorescent nanoparticle dispersion; take 50 μL of fluorescent nanoparticle dispersion in a test tube, and then add 100 μL different concentrations of Hg 2+ Aqueous solution, dilute to 250 μL with ultrapure water, and mix evenly so that the Hg in the final mixed solution 2+ The concentrations were 0, 0.08, 0.40, 0.64, 0.80, 1.60, 4.00, 6.40, 8.00, 16.00 and 24.00 μmol / L, and then incubated at room temperature for 30 minutes and shaken evenly, using a PE LS55 fluorescence spectrophotometer (fluorescence conditions: excitation wavelength 313nm, the range of emission spectrum is 340~550nm, the slit width of excitation and emission is 5nm, 10nm respectively) to detect different concentrations of ...

Embodiment 3

[0048] 1. Disperse 0.0034 mg of imine-linked fluorescent nanoparticles in 1 mL of phosphate buffer with a pH value of 7.5 to prepare a 3.4 μg / mL fluorescent nanoparticle dispersion; take 50 μL of fluorescent nanoparticle dispersion in a test tube , followed by adding 50 μL of 100.00 μmol / L Hg 2+ aqueous solution and 50 μL of 100.00 μmol / L iodothioacetylcholine aqueous solution, and 50 μL of acetylcholinesterase aqueous solutions with different activities were added, and 50 μL of phosphate buffer solution with a pH value of 7.5 was used to make up to 250 μL, and mixed evenly to make the mixed solution The activities of acetylcholinesterase in the medium were 0, 1.33, 6.67, 13.33, 33.33, 40.00, 46.67, 53.33mU / mL, and then incubated at room temperature for 30min and shaken evenly, and detected with a PE LS55 fluorescence spectrophotometer (fluorescence conditions: excitation wavelength 313nm, the scope of the emission spectrum is 340~550nm, the slit width of excitation and emissi...

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Abstract

The invention discloses fluorescent nanoparticles based on imine connection and application thereof to detection of Hg<2+> and acetylcholinesterase. The fluorescent nanoparticles are prepared by a simple room temperature synthesis method by using 3, 5-diaminobenzoic acid and 1, 3, 5-benzenetricarboxaldehyde as monomers. With the fluorescent nanoparticles as a fluorescent probe, a method for analyzing a trace amount of Hg<2+> in water is established and a method for detecting the acetylcholinesterase is established. Compared with other fluorescent probes, the fluorescent nanoparticles not only have relatively high absolute fluorescence quantum yield and uniform spherical particle size distribution, but also have relatively good crystallinity, uniform pore size distribution and good thermal stability, and high in selectivity, high in accuracy and good in precision during the detection of the Hg<2+> and the acetylcholinesterase.

Description

technical field [0001] The invention belongs to Hg 2+ and the detection technical field of acetylcholinesterase, specifically relate to a fluorescent nanoparticle based on imine connection, and use the nanoparticle as a fluorescent probe to detect Hg 2+ and acetylcholinesterase. Background technique [0002] Mercury, commonly known as mercury, often exists in liquid form and is a heavy metal. Mercury is a highly toxic and non-essential element. It is the only heavy metal that can cycle perfectly in the ecosystem, and it will eventually be delivered to the human body through the complementary expression of the food chain. Mercury ions can bind to rare bases and interfere with cellular metabolism and function. In addition, mercury can damage the central nervous system of the human body, and long-term activities in a high-mercury environment can cause brain damage or even death. Mercury mainly exists in nature in the form of metallic mercury, inorganic mercury and organic m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C07C249/02C07C251/24G01N21/64B82Y20/00B82Y40/00
CPCB82Y20/00B82Y40/00C07C249/02C09K11/06C09K2211/1014G01N21/643C07C251/24
Inventor 张静魏静茹张微李书茗
Owner SHAANXI NORMAL UNIV
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