Streptomyces avermitilis synthetic medium and preparation method of streptomyces avermitilis fermentation broth

A technology for synthesizing culture medium and Streptomyces avermitilis, which is applied in the field of microbial culture medium and fermentation, can solve the problems that affect the quantitative and qualitative analysis of experimental results, complex components, etc., and achieve the effect of clear medium composition, rich nutrition and reasonable formula

Inactive Publication Date: 2017-11-10
TIANJIN UNIV OF SCI & TECH +1
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex ingredients of bean cake powder and peanut cake powder, there are differences between different production areas and different batches, which affects the quantitative and qualitative analysis of the experimental results.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] A preparation method for Streptomyces avermitilis fermented liquid, comprises the steps:

[0032] S1. Seed cultivation: The medium formula is (g / L): corn starch 30, soybean cake powder 8, peanut cake powder 10, yeast powder 4, amylase 0.03, cobalt chloride 0.003; The components were boiled for 15 minutes, cooled at room temperature and filtered with 10 layers of medical gauze to remove residue; conditions: initial pH = 6.86-6.90, cultured on a shaker at 240 rpm at 28°C for 40 hours, centrifuged at 4000 rpm at room temperature for 5 minutes in a 50 mL centrifuge tube, discarded the supernatant and used Wash and centrifuge 3 times with equal volume of sterile normal saline, suspend with equal volume of sterile normal saline;

[0033]S2. Prepare a culture medium for abamectin-producing Streptomyces avermitilis strains, the medium formula is (g / L): maltose monohydrate 8-25, anhydrous glucose 3-14, threonine 0.8-2.5, heptahydrate Magnesium sulfate 0.3~0.5, ferrous sulfate h...

Embodiment 1

[0036] Strain: Streptomyces avermitilis (Streptomyces avermitilis) 9-39 strain was provided by the Institute of Microbiology, Chinese Academy of Sciences.

[0037] The composition of the fermentation synthetic medium used is (g / L): 8 maltose monohydrate, 3 glucose anhydrous, 0.8 threonine, 0.3 magnesium sulfate heptahydrate, 0.005 ferrous sulfate heptahydrate, 0.15 dipotassium hydrogen phosphate trihydrate, Manganese sulfate water 0.01, sodium chloride 0.3, MOPS4.

[0038] First, after activating the preserved Streptomyces avermitilis strain to obtain a single colony, inoculate it into a 250mL Erlenmeyer flask with 40mL filtered seed medium, and continuously cultivate it at a constant temperature of 240rpm in a shaker at 28°C for 40h to obtain Streptomyces avermitilis The liquid seeds are cleaned and suspended; the liquid seeds are transferred to the high-yield liquid medium according to the inoculum size of 5%, and the active substances of Streptomyces avermitilis strains can...

Embodiment 2

[0040] Strain: Streptomyces avermitilis (Streptomyces avermitilis) 9-39 strain was provided by the Institute of Microbiology, Chinese Academy of Sciences.

[0041] The composition of the fermentation synthetic medium used is (g / L): 16 maltose monohydrate, 8 anhydrous glucose, 1.6 threonine, 0.3 magnesium sulfate heptahydrate, 0.015 ferrous sulfate heptahydrate, 0.45 dipotassium hydrogen phosphate trihydrate, Manganese sulfate water 0.02, sodium chloride 0.6, MOPS6.

[0042] First, after activating the preserved Streptomyces avermitilis strain to obtain a single colony, inoculate it into a 250mL Erlenmeyer flask with 40mL filtered seed medium, and continuously cultivate it at a constant temperature of 240rpm in a shaker at 28°C for 40h to obtain Streptomyces avermitilis The liquid seeds are cleaned and suspended; the liquid seeds are transferred to the high-yield liquid medium according to the inoculation amount of 7%, and the active substances of Streptomyces avermitilis strai...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a streptomyces avermitilis synthetic medium. The streptomyces avermitilis synthetic medium comprises the following raw materials by weight according to 1L volume: 8-25 g of maltose monohydrate, 3-14 g of anhydrous dextrose, 0.8-2.5 g of threonine, 0.3-0.5 g of magnesium sulfate heptahydrate, 0.005-0.015 g of iron vitriol, 0.15-0.45 g of dipotassium phosphate trihydrate, 0.01-0.02 g of manganese sulfate monohydrate, 0.3-0.6 g of sodium chloride, 4-6 g of 3-(4-morpholino)propanesulphonic acid and the balance of water. The invention discloses a preparation method of a streptomyces avermitilis fermentation broth. The streptomyces avermitilis synthetic medium is reasonable in formula and rich in nutrition, meets the growth and avermectins production requirements of streptomyces avermitilis, and is suitable for related basic scientific study, and the abamectin B1a effective component is 2.84 times of that of a clear medium.

Description

technical field [0001] The invention belongs to the technical field of microbial culture medium and fermentation, and relates to a synthetic culture medium of Streptomyces avermitilis and a preparation method thereof. Background technique [0002] Streptomyces avermitilis (Streptomyces avermitilis) is the production bacterium of abamectin, is isolated from the soil of Shizuoka Prefecture, Japan in 1978, and by fermenting and culturing the Streptomyces avermitilis strain, it is found that its fermented liquid has a high Anthelmintic activity, then this strain was sent to Merck, USA for further research. In 2002, Kitasato Research Institute in Japan conducted in-depth research on the morphology, physiological characteristics and phylogenetic evolution of this strain, and finally renamed it Streptomyces avermectinius. Streptomyces avermitilis belongs to the Gram-positive bacteria of the kingdom Prokaryotes, the phylum Actinobacteria, the class Actinomycetes, the suborder Strep...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/62C12R1/465
CPCC12N1/20C12P19/623
Inventor 高强张立新胡栋张敬宇谭高翼
Owner TIANJIN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap