Streptomyces avermitilis synthetic medium and preparation method of streptomyces avermitilis fermentation broth
A technology for synthesizing culture medium and Streptomyces avermitilis, which is applied in the field of microbial culture medium and fermentation, can solve the problems that affect the quantitative and qualitative analysis of experimental results, complex components, etc., and achieve the effect of clear medium composition, rich nutrition and reasonable formula
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[0031] A preparation method for Streptomyces avermitilis fermented liquid, comprises the steps:
[0032] S1. Seed cultivation: The medium formula is (g / L): corn starch 30, soybean cake powder 8, peanut cake powder 10, yeast powder 4, amylase 0.03, cobalt chloride 0.003; The components were boiled for 15 minutes, cooled at room temperature and filtered with 10 layers of medical gauze to remove residue; conditions: initial pH = 6.86-6.90, cultured on a shaker at 240 rpm at 28°C for 40 hours, centrifuged at 4000 rpm at room temperature for 5 minutes in a 50 mL centrifuge tube, discarded the supernatant and used Wash and centrifuge 3 times with equal volume of sterile normal saline, suspend with equal volume of sterile normal saline;
[0033]S2. Prepare a culture medium for abamectin-producing Streptomyces avermitilis strains, the medium formula is (g / L): maltose monohydrate 8-25, anhydrous glucose 3-14, threonine 0.8-2.5, heptahydrate Magnesium sulfate 0.3~0.5, ferrous sulfate h...
Embodiment 1
[0036] Strain: Streptomyces avermitilis (Streptomyces avermitilis) 9-39 strain was provided by the Institute of Microbiology, Chinese Academy of Sciences.
[0037] The composition of the fermentation synthetic medium used is (g / L): 8 maltose monohydrate, 3 glucose anhydrous, 0.8 threonine, 0.3 magnesium sulfate heptahydrate, 0.005 ferrous sulfate heptahydrate, 0.15 dipotassium hydrogen phosphate trihydrate, Manganese sulfate water 0.01, sodium chloride 0.3, MOPS4.
[0038] First, after activating the preserved Streptomyces avermitilis strain to obtain a single colony, inoculate it into a 250mL Erlenmeyer flask with 40mL filtered seed medium, and continuously cultivate it at a constant temperature of 240rpm in a shaker at 28°C for 40h to obtain Streptomyces avermitilis The liquid seeds are cleaned and suspended; the liquid seeds are transferred to the high-yield liquid medium according to the inoculum size of 5%, and the active substances of Streptomyces avermitilis strains can...
Embodiment 2
[0040] Strain: Streptomyces avermitilis (Streptomyces avermitilis) 9-39 strain was provided by the Institute of Microbiology, Chinese Academy of Sciences.
[0041] The composition of the fermentation synthetic medium used is (g / L): 16 maltose monohydrate, 8 anhydrous glucose, 1.6 threonine, 0.3 magnesium sulfate heptahydrate, 0.015 ferrous sulfate heptahydrate, 0.45 dipotassium hydrogen phosphate trihydrate, Manganese sulfate water 0.02, sodium chloride 0.6, MOPS6.
[0042] First, after activating the preserved Streptomyces avermitilis strain to obtain a single colony, inoculate it into a 250mL Erlenmeyer flask with 40mL filtered seed medium, and continuously cultivate it at a constant temperature of 240rpm in a shaker at 28°C for 40h to obtain Streptomyces avermitilis The liquid seeds are cleaned and suspended; the liquid seeds are transferred to the high-yield liquid medium according to the inoculation amount of 7%, and the active substances of Streptomyces avermitilis strai...
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