A construction method of encoder and decoder

A construction method and decoder technology, applied in instruments, scientific instruments, material excitation analysis, etc., can solve problems such as complex construction methods and unstable systems, and achieve the effects of simple construction, high sensitivity, and easy distinction

Active Publication Date: 2019-07-23
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a construction method of an encoder and a decoder, which can effectively solve the problems of complex construction methods and unstable systems in the prior art

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  • A construction method of encoder and decoder
  • A construction method of encoder and decoder
  • A construction method of encoder and decoder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Constructing a 2:1 type data encoder

[0043] Take 2 EP tubes, numbered 1 and 2, and do the following:

[0044] With the Tris-HCl buffer solution of pH=7.0-9.0 as the input signal, add a concentration of 100×10 - 3 mol / L cyanine dye Ⅰ solution and the concentration is 10×10 -3 mol / L, Tris-HCl buffer solution of pH=8.5, make the mixed solution volume be 1mL, obtain solution 1; Wherein, the concentration of cyanine dye I solution in solution 1 is 30×10 -6 mol / L.

[0045] Using potassium ions and Tris-HCl buffer solution with pH=7.0-9.0 as the input signal, add a concentration of 1×10 -3 mol / L KCl solution, the concentration is 10×10 -3 mol / L, Tris-HCl buffer solution with pH=8.5 and a concentration of 100×10 -3 mol / L cyanine dye I solution, so that the volume of the mixed solution is 1mL to obtain solution 2; wherein, the concentration of KCl solution in solution 2 is 80×10 -6 mol / L, the concentration of cyanine dye Ⅰ solution is 30×10 -6 mol / L.

[0...

Embodiment 2

[0051] Example 2 Constructing a 4:2 type data encoder

[0052] Take 4 EP tubes, numbered 1, 2, 3 and 4, and do the following:

[0053] With the Tris-HCl buffer solution of pH=7.0-9.0 as the input signal, add a concentration of 100×10 - 3 mol / L cyanine dye II solution and concentration of 10×10 -3 mol / L, Tris-HCl buffer solution of pH=8.5, make the mixed solution volume be 1mL, obtain solution 1; Wherein, the concentration of cyanine dye II solution in solution 1 is 30×10 -6 mol / L.

[0054] With the Tris-HCl buffer solution of pH=3.0-6.0 as the input signal, add the concentration of 100×10 - 3 mol / L cyanine dye II solution and concentration of 10×10 -3 mol / L, Tris-HCl buffer solution of pH=4.0, make the mixed solution volume be 1mL, obtain solution 2; Wherein, the concentration of cyanine dye II solution in solution 2 is 30×10 -6 mol / L.

[0055]Using potassium ions and Tris-HCl buffer solution with pH = 7.0-9.0 as the input signal, add a concentration of 1 × 10 -3 mol / ...

Embodiment 3

[0065] Embodiment 3 Construction of 8:3 type data encoder

[0066] Take 8 EP tubes, numbered 1, 2, 3, 4, 5, 6, 7 and 8, and do the following:

[0067] With the Tris-HCl buffer solution of pH=7.0-9.0 as the input signal, add a concentration of 100×10 - 3 mol / L cyanine dye III solution and concentration is 10×10 -3 mol / L, Tris-HCl buffer solution of pH=8.5, make the mixed solution volume be 1mL, obtain solution 1; Wherein, the concentration of cyanine dye III solution in solution 1 is 30×10 -6 mol / L.

[0068] With the Tris-HCl buffer solution of pH=3.0-6.0 as the input signal, add the concentration of 100×10 - 3 mol / L cyanine dye III solution and concentration is 10×10 -3 mol / L, Tris-HCl buffer solution of pH=4.0, make the mixed solution volume be 1mL, obtain solution 2; Wherein, the concentration of cyanine dye III solution in solution 2 is 30×10 -6 mol / L.

[0069] Using potassium ions and Tris-HCl buffer solution with pH = 7.0-9.0 as the input signal, add a concentrati...

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Abstract

The invention provides a construction method for an encoder and a decoder. The method comprises the step of constructing the encoder or the decoder in the manner of taking a cyanine dye solution or cyanine dye solution and double-chain DNA as a template, taking H+, potassium ions, sodium ions and single-chain DNA as input factors, taking a Tris-HCl buffering solution in concentration of (1-100)*10<-3> mol / L and pH of 7.0-9.0 as an one-path input signal, taking the input factors or the combination thereof as parallel input signals and taking diploid or a cyanine dye aggregation state induced by the input factors as an output signal. The encoder and the decoder are high in sensitivity, are at high switch ratio and are easily distinguished; the threshold value is below 0.3; the output signal is easily detached; the dependency on the detecting instrument is less; an absorbing / fluorescence detector can be used for realizing detection; the ionic concentration in the solution can be efficiently, sensitively, simply and quickly detected.

Description

technical field [0001] The invention belongs to the technical field of encoders and decoders, and in particular relates to a construction method of an encoder and a decoder. Background technique [0002] The advancement of integrated technology has continuously upgraded traditional computers to high speed, small size and large storage. However, due to the complexity of integrated circuits, the storage limit of inorganic silicon chips, and the limitations of traditional computer computing methods, traditional computers have great difficulties in realizing ultra-microstructures, super-large storage, and increasing computing speed by orders of magnitude. The DNA chips brought about by the development of biotechnology are much better than traditional computers in the above aspects. This new type of computer based on the calculation model of DNA biochemical reactions is called DNA computer. [0003] In the DNA computer, the problem of the traditional computer is also faced, that...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33G01N21/64
CPCG01N21/33G01N21/6486
Inventor 杨千帆杨舒杨春容陈见驰苗家榕黄丹杜媛媛
Owner SICHUAN UNIV
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