Sequencing reaction cell, sequencing reaction clamp and sequencing reaction equipment
A technology for sequencing reaction chambers and sequencing reactions, applied in biochemical equipment and methods, bioreactor/fermenter combinations, specific-purpose bioreactor/fermenter, etc., can solve the problems of low temperature measurement accuracy, temperature influence, Temperature measurement is prone to problems such as deviation, so as to achieve the effect of improving accuracy
Active Publication Date: 2017-11-28
SHENZHEN HYK HIGH THROUGHPUT BIOTECH INST
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[0005] The purpose of the present invention is to provide a gene sequencing reaction chamber and gene sequencing reaction equipment, aiming to solve the problem that the air convection on the surface of the gene sequencing reaction chamber in the prior art is likely to affect the measurement of temperature, resulting in deviations in temperature measurement. The problem of low measurement accuracy
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[0047] The present invention proposes the first embodiment. This embodiment proposes a sequencing reaction chamber. By fixing a plurality of DNA fragments in the sequencing reaction chamber, the reaction reagents flow through the DNA fragments to realize the gene sequencing reaction between the reaction reagents and the DNA fragments. . Such as figure 1 , Figure 4 As shown, the sequencing reaction chamber includes a first substrate 10 and a second substrate 20, and the lower surface of the first substrate 10 is attached to the upper surface of the second substrate 20 to realize the first substrate 10 and the sealing between the second substrate 20; at least one hollow reaction channel is formed between the first substrate 10 and the second substrate 20, and when there is only one reaction channel, single-channel gene sequencing is realized For the reaction, when there are multiple reaction channels, parallel sequencing of multiple channels can be realized. The reaction chan...
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The invention relates to the field of gene sequencing and provides a sequencing reaction cell, a sequencing reaction clamp and sequencing reaction equipment. The sequencing reaction cell comprises a first substrate and a second substrate, wherein the lower surface of the first substrate fits the upper surface of the second substrate, and at least one hollow reaction channel is formed between the first substrate and the second substrate; a reagent outlet is formed in each of the first substrate and the second substrate; a heating slide for heating a reagent in the reaction channel fits the upper surface of the first substrate; a bump corresponding to the reaction channel is formed on the second substrate, a temperature measuring hole is formed in the bump, and a temperature measuring probe is accommodated in the temperature measuring hole. The sequencing reaction cell provided by the invention has the beneficial effects of preventing influence on temperature measurement by cross-ventilation of the surface of the gene sequencing reaction cell and improving the accuracy of temperature measurement of a gene sequencing reaction, so that the measured temperature is closer to the temperature of the reaction reagent of the gene sequencing reaction.
Description
technical field [0001] The invention relates to the field of gene sequencing, and more specifically, the invention relates to a sequencing reaction chamber, a sequencing reaction fixture and sequencing reaction equipment. Background technique [0002] In the prior art, a sequencing reaction device includes a sequencing reaction chamber, a temperature control device, and a temperature detection device. The temperature control device generally includes a refrigerating sheet, and the refrigerating sheet is in contact with the sequencing reaction chamber, and the sequencing reaction chamber is controlled by the refrigerating sheet. The temperature is controlled; the temperature detection device generally includes a temperature sensor, and the temperature sensor is also in contact with the sequencing reaction chamber, and the temperature of the sequencing reaction is obtained by detecting the temperature of the sequencing reaction chamber. [0003] This device for detecting the t...
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Login to View More IPC IPC(8): C12M1/34
CPCC12Q1/6869
Inventor 盛司潼祝捷
Owner SHENZHEN HYK HIGH THROUGHPUT BIOTECH INST