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A cassava nutrient solution for efficient propagation of Tetranychus cinnabarinus

A technology of Tetranychus cinnabarinus and nutrient solution, applied in animal feed, additional food elements, applications, etc., can solve the problems of plant death, affecting yield and quality, and easy breeding and reproduction of diseases and insect pests, so as to accelerate the reproduction of offspring, increase the reproduction rate, The effect of improving reproductive efficiency

Inactive Publication Date: 2020-08-14
SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the climate in Guangxi is humid and warm, and there are many varieties of economic crops in the tropics and subtropics, and pests and diseases are easy to breed.
Cinnabar spider mite [Tetranychus cinnabarinus (Boisduval)] belongs to the Tetranychidae family (Tetranychidae), commonly known as red spider, is the most serious pest of cassava, and can cause the death of the whole plant in severe cases, thereby affecting the yield and quality.

Method used

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  • A cassava nutrient solution for efficient propagation of Tetranychus cinnabarinus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] One, the preparation of cassava nutrient solution, wherein the composition of every liter of this nutrient solution and the content of each component are as follows:

[0024] 1. Trace elements: CaCl 2 ·H 2 O 0.025mg / L; CuSO 4 ·5H 2 O 0.035mg / L; FeNaEDTA 4.25mg / L; MnSO 4 ·H 2 O 1.20mg / L; Na 2 MoO 4 2H 2 O 0.035mg / L; ZnSO 4 ·H 2 O 0.45mg / L

[0025] 2. Amino acids: glutamine 0.5g / L; lysine 0.10g / L; glycine 0.2g / L; proline 0.1g / L

[0026] 3. Vitamins: biotin 0.2g / L; niacin 0.1g / L; pyridoxine hydrochloride 0.25mg / L; vitamin B2 0.15mg / L

[0027] 4. Sucrose: 20g / L

[0028] 5. Preparation of nutrient solution: add separately according to the content of each component, the order is trace element-amino acid-vitamin-sucrose-add water to volume-adjust pH value to 6.8.

[0029] 6. Take a few pieces of semi-mature and clean cassava leaves of insect-susceptible varieties, homogenize and centrifuge, mix the supernatant with the above-mentioned nutrient solution at a ratio ...

Embodiment 2

[0041]One, the preparation of cassava nutrient solution, wherein the composition of every liter of this nutrient solution and the content of each component are as follows:

[0042] 1. Trace elements: CaCl 2 ·H 2 O 0.02mg / L; CuSO 4 ·5H 2 O 0.03mg / L; FeNaEDTA 4.0mg / L; MnSO 4 ·H 2 O 1.0mg / L; Na 2 MoO 4 2H 2 O 0.03mg / L; ZnSO 4 ·H 2 O 0.4mg / L

[0043] 2. Amino acids: glutamine 0.3g / L; lysine 0.10g / L; glycine 0.3g / L; proline 0.2g / L

[0044] 3. Vitamins: biotin 0.4g / L; niacin 0.3g / L; pyridoxine hydrochloride 0.4mg / L; vitamin B2 0.3mg / L

[0045] 4. Sucrose: 22g / L

[0046] 5. Preparation of nutrient solution: add separately according to the content of each component, the order is trace element-amino acid-vitamin-sucrose-add water to volume-adjust pH value to 6.8.

[0047] 6. Take a few pieces of semi-mature and clean cassava leaves of insect-susceptible varieties, homogenize and centrifuge, mix the supernatant with the above-mentioned nutrient solution at a ratio of 2:1, f...

Embodiment 3

[0054] One, the preparation of cassava nutrient solution, wherein the composition of every liter of this nutrient solution and the content of each component are as follows:

[0055] 1. Trace elements: CaCl 2 ·H 2 O 0.05mg / L; CuSO 4 ·5H 2 O 0.05mg / L; FeNaEDTA 5.0mg / L; MnSO 4 ·H 2 O 1.5mg / L; Na 2 MoO 4 2H 2 O 0.05mg / L; ZnSO 4 ·H 2 O 0.5mg / L

[0056] 2. Amino acids: glutamine 0.6g / L; lysine 0.20g / L; glycine 0.1g / L; proline 0.1g / L

[0057] 3. Vitamins: biotin 0.3g / L; niacin 0.2g / L; pyridoxine hydrochloride 0.2mg / L; vitamin B2 0.1mg / L

[0058] 4. Sucrose: 18g / L

[0059] 5. Preparation of nutrient solution: add separately according to the content of each component, the order is trace element-amino acid-vitamin-sucrose-add water to volume-adjust pH value to 6.8.

[0060] 6. Take a few semi-mature and clean cassava leaves of insect-susceptible varieties, homogenize and centrifuge, mix the supernatant with the above-mentioned nutrient solution at a ratio of 1.5:1, filter t...

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Abstract

The invention provides a cassava nutrient solution for highly-efficiently breeding Tetranychus cinnabarinus. The cassava nutrient solution is prepared through the following steps: homogenating clean insect-susceptible variety cassava leaves, centrifuging the obtained slurry, mixing the obtained supernatant with a nutrient solution according to a ratio of (1-2):1, filtering the obtained mixture, packaging the filtered mixture, and storing the packaged mixture at -20 DEG C for later use. The nutrient solution consists of several trace elements, amino acids, vitamins and sucrose. The cassava nutrient solution is used to breed the Tetranychus cinnabarinus in order to obtain a large amount of Tetranychus cinnabarinus in a short time, improve the early survival rate of the Tetranychus cinnabarinus, prolong the survival time and accelerate the progeny propagation. The Tetranychus cinnabarinus reproduction rate can reach 85% or more, and is 30% or more higher than that of nutrient solution-free breeding methods. The cassava nutrient solution is used to carry out indoor rapid propagation of Tetranychus cinnabarinus in order to provide a large number of Tetranychus cinnabarinus sources for the development of resistance breeding test of the Tetranychus cinnabarinus.

Description

technical field [0001] The invention belongs to the field of biological control of agricultural pests, and in particular relates to a cassava nutrient solution for efficiently propagating Tetranychus cinnabarinus. Background technique [0002] Guangxi is the largest cassava planting base and starch processing base in the country. Cassava production has become an important pillar industry in Guangxi, a new growth point for Guangxi's economic development and an important way for farmers to increase their income. However, the climate in Guangxi is humid and warm, and there are many varieties of economic crops in the tropics and subtropics, and pests and diseases are easy to breed. Cinnabar spider mite [Tetranychus cinnabarinus (Boisduval)] belongs to Tetranychidae (Tetranychidae), commonly known as spider mite, is the most serious pest of cassava, and can cause the death of the whole plant in severe cases, thereby affecting the yield and quality. Especially in recent years, wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/90A23K20/24A23K20/20A23K20/142A23K20/174A23K20/163
CPCA23K20/142A23K20/163A23K20/174A23K20/24A23K20/30A23K50/90
Inventor 刘连军黎萍陈海生李恒锐杨海霞农秋连马仙花梁振华谢君锋青鑫韦雪英郭素云林嘉雯
Owner SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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