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Carbofuran and isoprocarb double joint inspection immune fluorescent test paper strip, kit and application thereof

A technology of immunofluorescence and isoprocarb, applied in biological testing, material inspection products, measuring devices, etc., can solve problems such as unscientific, harmful, human and animal poisoning, and achieve the effects of simple operation, improved sensitivity, and high specificity

Active Publication Date: 2017-12-12
SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the unscientific use of carbamate pesticides in large quantities, the phenomenon of human and animal poisoning has occurred from time to time. Eating contaminated food for a long time or being exposed to its environment will cause great harm to the human body.

Method used

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  • Carbofuran and isoprocarb double joint inspection immune fluorescent test paper strip, kit and application thereof
  • Carbofuran and isoprocarb double joint inspection immune fluorescent test paper strip, kit and application thereof
  • Carbofuran and isoprocarb double joint inspection immune fluorescent test paper strip, kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] In this example, the preparation of reagents related to the preparation of carbofuran and isoprocarb dual detection immunofluorescence test strips is as follows:

[0052] (1) Preparation and identification of carbofuran hapten KBW-1 / KBW-2

[0053] After 0.8g of furanol was dissolved in 15mL of dichloromethane, 0.58mL (10mmol) of pyridine was added to form a mixed solution, and 1g of p-nitrobenzene chloroformate solution (dissolved in 10mL of dichloromethane) was added dropwise, and the whole system was kept at 4°C The following ice salt bath. The reaction was followed by thin-plate chromatography (TLC), and it was determined that the reaction was completed within 3 h. The reaction solution was shaken and washed with 3% hydrochloric acid in a separatory funnel, and purified through a column to obtain light yellow crystals.

[0054] Get 0.4g (1.2mmol) the product that last step makes is dissolved in 12mL tetrahydrofuran, 0.16g (2.4mmol) 4-aminobutyric acid is dissolved ...

Embodiment 2

[0078] In this example, the reagents and raw materials prepared in Example 1 were used to prepare the carbofuran and isoprocarb dual detection immunofluorescence kit, which included test strips and cuvettes, and the test strips were as follows: figure 1 As shown, including bottom plate (7), sample pad (1), nitrocellulose membrane (2) and absorbent pad (3), the sample pad (1), nitrocellulose membrane (2) and absorbent pad (3) Adhere to the base plate (7) in sequence, and the nitrocellulose membrane (2) has the first detection line (4) bound to the carbofuran hapten-carrier protein conjugate sequentially from front to back according to the sample flow direction (i.e. T1 line), the second detection line (5) (i.e. T2 line) combined with isoprocarb hapten-carrier protein conjugate and the quality control line (6) (i.e. The quality control line (C line) in the present invention; the cuvette is equipped with carbofuran fluorescent microsphere-labeled antibody and isoprocarb fluoresce...

Embodiment 3

[0091] In this example, the Carbofuran and Isoprocarb Double Detection Immunofluorescence Kit prepared in Example 2 was used for sample detection. The steps are:

[0092] (1) Sample pretreatment: Take 2g of a chopped Chinese herbal medicine sample and add it to a 25mL centrifuge tube, add 100μL each of carbofuran and isoprocarb at a concentration of 10.0mg / L, and then add 8mL containing The extract of PBS buffer solution with a pH value of 10% ethanol is 7.2, which is the test solution after being fully oscillated and mixed; the negative control is added with 100 μL of the extract solution of PBS buffer solution of 7.2, and other operations remain unchanged.

[0093] (2) Detection: Pipette 200 μL (9-10 drops) of the above-mentioned solution to be tested into the cuvette, and pump up and down 10 times to mix well. Start the first step reaction at 20-40°C, and time it for 3 minutes; insert the test strip into the reaction cup; start the second step reaction at 20-40°C, and time ...

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Abstract

The invention provides a carbofuran and isoprocarb double joint inspection immune fluorescent test paper strip, a kit and application thereof. The test paper strip comprises a bottom plate, a sample pad, a nitrocellulose membrane and a water-absorbing pad; the sample pad, the nitrocellulose membrane and the water-absorbing pad are adhered to the bottom plate sequentially; a first detection line bound with a carbofuran hapten-vector protein conjugate, a second detection line bound with an isoprocarb hapten-vector protein conjugate and a quality control line bound with a goat anti-mouse polyclonal antibody are arranged on the nitrocellulose membrane sequentially from front to back according to the sample flowing direction; and the kit comprises the test paper strip and a reaction cup filled with a carbofuran fluorescent microsphere marked antibody and an isoprocarb fluorescent microsphere marked antibody. The carbofuran and isoprocarb double joint inspection immune fluorescent kit can realize detection of carbofuran and isoprocarb residues simultaneously, has high detection accuracy, has the characteristics of high sensitivity and high specificity, can realize simple and rapid detection, and is simple and convenient to operate and suitable for large-scale screening.

Description

technical field [0001] The invention belongs to the technical field of pesticide residue detection, and relates to an immunofluorescence test strip and a kit for dual detection of carbocarb and isoprocarb and an application thereof. Background technique [0002] Carbamate pesticides are high-efficiency, broad-spectrum insecticides, and their insecticidal mechanism is to inhibit the activity of acetylcholinesterase, an important substance in insect nerve transmission. Carbamate pesticides have the characteristics of remarkable insecticidal effect, fast decomposition, short residual period and rapid metabolism. In addition, it also has the effect of significantly stimulating the growth of crops. Due to the unscientific use of carbamate pesticides in large quantities, human and animal poisoning occurs from time to time. Eating contaminated food for a long time or being exposed to its environment will cause great harm to human health. Therefore, many countries and regions have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/544G01N33/58
CPCG01N33/544G01N33/558G01N33/582
Inventor 殷果秦斌王炳志杨星星闫研付辉王铁杰严义勇
Owner SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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