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An antibacterial peptide electrochemiluminescence sensor and its preparation method and detection method

An antibacterial peptide, electrochemical technology, applied in the field of biosensing technology and electrochemistry, can solve the problems of difficult to achieve online analysis, high operator requirements, cumbersome sample pretreatment, etc., to achieve accelerated electron transfer rate, strong anti-interference ability , to achieve the effect of online analysis

Active Publication Date: 2017-04-05
芒特拓普科技(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when these methods are applied, the sample pretreatment is cumbersome, the required instruments are expensive and bulky, the detection time is long, and the requirements for operators are high, so it is difficult to realize online analysis

Method used

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  • An antibacterial peptide electrochemiluminescence sensor and its preparation method and detection method
  • An antibacterial peptide electrochemiluminescence sensor and its preparation method and detection method
  • An antibacterial peptide electrochemiluminescence sensor and its preparation method and detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0062] A method for preparing an antibacterial peptide electrochemiluminescence sensor, comprising the following steps:

[0063] 1. Provide a bare gold electrode, place the bare gold electrode in 5 mL of Cys solution (containing 0.1mol / L HCl) with a concentration of 20mmol / L, and use cyclic voltammetry to electroplate an L-cysteine ​​layer on the surface of the bare gold electrode, The voltage is -0.5 ~ 1.0V, the scanning speed is 10mV / s, and the electroplating time is 20min; then soak and clean the electrode in water to remove unreacted Cys molecules on the surface to obtain an electrode modified with an L-cysteine ​​layer;

[0064] 2. Drop 40 μL of nano-gold dispersion with a particle size of about 10 nm on the surface of the electrode modified by the above-mentioned L-cysteine ​​layer, and react at 4°C for 20 h to prepare a nano-gold layer on the surface of the L-cysteine ​​layer , to obtain the electrode modified by nano-gold layer and L-cysteine ​​layer;

[0065] The pre...

Embodiment 2

[0072] A method for preparing an antibacterial peptide electrochemiluminescence sensor, comprising the following steps:

[0073] 1. Provide a bare gold electrode, place the bare gold electrode in 5mL of Cys solution (containing 0.1mol / L HCl) with a concentration of 15mmol / L, and use cyclic voltammetry to electroplate an L-cysteine ​​layer on the surface of the bare gold electrode, The voltage is -0.5 ~ 1.0V, the scanning speed is 5mV / s, and the electroplating time is 30min; then soak and clean the electrode in water to remove unreacted Cys molecules on the surface, and obtain an electrode modified with an L-cysteine ​​layer;

[0074] 2. Drop 40 μL of nano-gold dispersion with a particle size of about 10 nm on the surface of the electrode modified by the above-mentioned L-cysteine ​​layer, and react at 4°C for 22 hours to prepare a nano-gold layer on the surface of the L-cysteine ​​layer , to obtain the electrode modified by nano-gold layer and L-cysteine ​​layer;

[0075]3. D...

Embodiment 3

[0078] A method for preparing an antibacterial peptide electrochemiluminescence sensor, comprising the following steps:

[0079] 1. Provide a bare gold electrode, place the bare gold electrode in 5 mL of Cys solution (containing 0.1mol / L HCl) with a concentration of 25mmol / L, and use cyclic voltammetry to electroplate an L-cysteine ​​layer on the surface of the bare gold electrode, The voltage is -0.5 ~ 1.0V, the scanning speed is 15mV / s, and the electroplating time is 25min; then soak and clean the electrode in water to remove unreacted Cys molecules on the surface to obtain an electrode modified with an L-cysteine ​​layer;

[0080] 2. Drop 40 μL of nano-gold dispersion with a particle size of about 7 nm on the surface of the electrode modified by the above-mentioned L-cysteine ​​layer, and react at 4°C for 24 hours to prepare a nano-gold layer on the surface of the L-cysteine ​​layer , to obtain the electrode modified by nano-gold layer and L-cysteine ​​layer;

[0081] 3. D...

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Abstract

The invention provides an antimicrobial peptide electrochemiluminescence sensor which comprises a bare gold electrode, and an L-cysteine layer, a nano-gold layer and an antimicrobial peptide antibody layer which are stacked on the surface of the bare gold electrode in sequence, wherein the antimicrobial peptide antibody layer and the nano-gold layer are combined together through electrostatic adsorption. The antimicrobial peptide electrochemiluminescence sensor is small in size and low in cost, and can sensitively and quickly detect antimicrobial peptide on line. The invention provides a preparation method for the antimicrobial peptide electrochemiluminescence sensor. The preparation method for the antimicrobial peptide electrochemiluminescence sensor is simple and easy to operate. The invention also provides application of the antimicrobial peptide electrochemiluminescence sensor. The electrochemiluminescence sensor is high in stability, repetitiveness and specificity.

Description

technical field [0001] The invention relates to the fields of biosensing technology and electrochemical technology, in particular to an antibacterial peptide electrochemical luminescence sensor and its preparation method and detection method. Background technique [0002] Antimicrobial peptide is a kind of small molecular polypeptide with various biological activities that widely exists in organisms. It has the advantages of thermal stability, broad-spectrum antibacterial and potential anti-tumor properties, so it is widely used in clinical medicine, medicine, biological feed Additives, natural food preservatives and animal and plant disease resistance genetic engineering and other fields. The activity, mechanism of action, production of antimicrobial peptides and quality control of antimicrobial peptide products have become hot issues in the research of antimicrobial peptides, and the research on these issues urgently needs accurate, sensitive and simple quantitative detect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 栾崇林代建国
Owner 芒特拓普科技(深圳)有限公司
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