Fruit and vegetable fungicide containing camellia oleifera cake extracting solution and application of fruit and vegetable fungicide
A technology of camellia oleifera cake and extract, applied in the direction of bactericide, application, biocide, etc., can solve the problems of reducing the amount of prochloraz used, small sterilization range, food safety risks, etc., to reduce waste of resources and improve utilization rate , increase the effect of broad spectrum
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[0023] The preparation method of the camellia oleifera cake extract is as follows: take a certain amount of camellia oleifera cake, add the extractant according to the material-liquid ratio of 1: (10-20) kg / L, ultrasonically extract at 50-80°C for 1-4 hours, After extracting twice, the extracts were combined and concentrated in vacuum at 40-70°C;
[0024] The extractant is one or a mixture of acetone, methanol and ethanol, and the concentration is 60-90%.
[0025] The above-mentioned a kind of fruit and vegetable fungicide containing oil-tea oleifera cake extract has the following ratios:
[0026] Camellia oleifera cake extract: Trichoderma inoculum is (1~3): (3~1);
[0027] Camellia oleifera cake extract: natamycin is (1~5): (1~3);
[0028] Camellia oleifera cake extract: nisin is (1~3): (1~4);
[0029] Camellia oleifera cake extract: Trichoderma agent: Nisin is (1~3): (2~3): (1~5);
[0030] Camellia oleifera cake extract: natamycin: nisin (2~5): (2~3): (1~5); use after d...
Embodiment 1
[0033] Weigh about 2 g of tea seed cake, put it in a Soxhlet extractor, add water, 70% methanol, ethanol, and acetone at a ratio of 1:10 g / mL, and extract 1 h with ultrasonic assistance at 60 °C, extract twice, and extract Finally, after recovering the solvent under reduced pressure using a circulating pump, the extract concentrate was obtained, dissolved in 70% ethanol solution, and the contents of saponin, total phenols and polysaccharides in different extracts were analyzed, and the results are shown in figure 1 .
Embodiment 2
[0035] The test materials and steps are the same as in Example 1, wherein the concentration of extractant is 50%, the extraction temperature is 30°C, the ratio of solid to liquid is 1:15 g / ml, and the extraction time is 3h. The contents of saponin, total phenols and polysaccharides in different extracts were analyzed, and the results are shown in Table 1.
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