Fruit and vegetable fungicide containing camellia oleifera cake extracting solution and application of fruit and vegetable fungicide

A technology of camellia oleifera cake and extract, applied in the direction of bactericide, application, biocide, etc., can solve the problems of reducing the amount of prochloraz used, small sterilization range, food safety risks, etc., to reduce waste of resources and improve utilization rate , increase the effect of broad spectrum

Inactive Publication Date: 2017-12-15
GUIYANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Prior art CN 101543242 B found that Camellia oleifera extract has good control effect on some molds and anthracnose, but because Camellia oleifera extract has a small bactericidal range, it is only used in citrus, litchi, banana, loquat, longan, mango and other fruits P

Method used

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  • Fruit and vegetable fungicide containing camellia oleifera cake extracting solution and application of fruit and vegetable fungicide
  • Fruit and vegetable fungicide containing camellia oleifera cake extracting solution and application of fruit and vegetable fungicide
  • Fruit and vegetable fungicide containing camellia oleifera cake extracting solution and application of fruit and vegetable fungicide

Examples

Experimental program
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Effect test

preparation example Construction

[0023] The preparation method of the camellia oleifera cake extract is as follows: take a certain amount of camellia oleifera cake, add the extractant according to the material-liquid ratio of 1: (10-20) kg / L, ultrasonically extract at 50-80°C for 1-4 hours, After extracting twice, the extracts were combined and concentrated in vacuum at 40-70°C;

[0024] The extractant is one or a mixture of acetone, methanol and ethanol, and the concentration is 60-90%.

[0025] The above-mentioned a kind of fruit and vegetable fungicide containing oil-tea oleifera cake extract has the following ratios:

[0026] Camellia oleifera cake extract: Trichoderma inoculum is (1~3): (3~1);

[0027] Camellia oleifera cake extract: natamycin is (1~5): (1~3);

[0028] Camellia oleifera cake extract: nisin is (1~3): (1~4);

[0029] Camellia oleifera cake extract: Trichoderma agent: Nisin is (1~3): (2~3): (1~5);

[0030] Camellia oleifera cake extract: natamycin: nisin (2~5): (2~3): (1~5); use after d...

Embodiment 1

[0033] Weigh about 2 g of tea seed cake, put it in a Soxhlet extractor, add water, 70% methanol, ethanol, and acetone at a ratio of 1:10 g / mL, and extract 1 h with ultrasonic assistance at 60 °C, extract twice, and extract Finally, after recovering the solvent under reduced pressure using a circulating pump, the extract concentrate was obtained, dissolved in 70% ethanol solution, and the contents of saponin, total phenols and polysaccharides in different extracts were analyzed, and the results are shown in figure 1 .

Embodiment 2

[0035] The test materials and steps are the same as in Example 1, wherein the concentration of extractant is 50%, the extraction temperature is 30°C, the ratio of solid to liquid is 1:15 g / ml, and the extraction time is 3h. The contents of saponin, total phenols and polysaccharides in different extracts were analyzed, and the results are shown in Table 1.

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Abstract

The invention discloses a fruit and vegetable fungicide containing a camellia oleifera cake extracting solution. Effective components are extracted from a camellia oleifera cake and are compounded with a biological fungicide; a mixture is applied to microbial disease and pest control over fruits and vegetables; the camellia oleifera cake extracting solution is compounded with one or several of a trichoderma agent, lactic streptococci and natamycin, and a mixture is diluted for use. The preparation method of the camellia oleifera cake extracting solution comprises the following steps: taking a certain amount of camellia oleifera cake, and adding an extracting agent according to the solid-liquid ratio of 1:(10 to 20) kg/L; carrying out ultrasonic extraction at the temperature of 50 to 80 DEG C for 1 to 4 hours, extracting for two times, then combining the extracting solution, and carrying out vacuum concentration at the temperature of 40 to 70 DEG C, wherein the extracting agent is one or a mixture of acetone, methanol and ethanol, and the concentration is 60 to 90 percent. The fruit and vegetable fungicide disclosed by the invention is formed by compounding a plant source with a biological source; the broad spectrum of the fungicide can be improved, drug resistance of pathogenic bacteria is overcome, the use amount of chemical pesticide can be effectively reduced, and the safety of fruit and vegetable products is improved, so the fruit and vegetable fungicide is a fungicide for effectively preventing and controlling microbial diseases and pests of the fruits and vegetables.

Description

technical field [0001] The invention belongs to the technical field of storage and fresh-keeping of agricultural products, and in particular relates to a fruit and vegetable fungicide containing tea-oleifera cake extract and application thereof. technical background [0002] Camellia oleifera is a plant of the family Theaceae Camellia. It is a unique woody edible oil economic forest tree species in my country. It is called the world's four major woody edible oil sources together with oil palm, olive and coconut. It is mainly distributed in southern my country, such as Hunan, Jiangxi, Guangxi, Zhejiang, Fujian, Anhui, Guizhou and other provinces. my country is a big producer of camellia oleifera seeds. According to the "National Camellia oleifera Industry Development Plan (2009~2020)" approved and issued by the State Council, by 2020 the total planting scale of camellia oleifera in my country will reach 4.6667 million hm2, and the output of camellia oleifera will reach 2.5 mil...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01N63/04A01N63/00A01N43/90A01P1/00A01P3/00A23B7/154A23B7/155
CPCA01N43/90A01N63/00A01N63/30A01N65/00A01N65/08A23B7/154A23B7/155A23V2002/00A23V2200/10A23V2250/214
Inventor 谢国芳杨飞燕张洁王玉波马立志
Owner GUIYANG UNIV
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