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A kind of immobilization method and application of horseradish peroxidase

A technology of horseradish peroxidase and mesoporous silica, applied in oxidoreductase, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of low recovery rate, easy inactivation, poor stability, etc. , to improve stability and improve the effect of application

Active Publication Date: 2020-08-11
FUZHOU UNIV
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Problems solved by technology

[0007] Aiming at the deficiencies of easy inactivation, poor stability, and low recovery rate of free enzymes in catalytic reactions, the present invention provides aminated mesoporous silica nanoparticles as immobilized carriers to achieve a large loading capacity and protect enzymes from catalysis. Activity, easy recovery and other characteristics, used for the removal of phenolic compounds in sewage

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  • A kind of immobilization method and application of horseradish peroxidase
  • A kind of immobilization method and application of horseradish peroxidase
  • A kind of immobilization method and application of horseradish peroxidase

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Embodiment 1

[0025] Firstly, mesoporous silica nanoparticles were synthesized according to the following method: mix 3.36mL ammonia water and 75mL ethanol evenly, add 0.21mL formaldehyde and 0.15g resorcinol after 5 minutes of reaction, and add 0.65mL of protophenol dropwise after 7 hours of reaction. Tetraethyl silicate. After the reaction, the obtained material was centrifuged and washed three times with ethanol, dried overnight in vacuum, and calcined at 550° C. for 6 hours to obtain mesoporous silica nanoparticles. The obtained mesoporous silica nanoparticles are white powder. 0.2 g of the obtained white powder was dissolved in ultrapure water, heated to boiling, reacted for 2 h, centrifuged, and dried in a vacuum oven. Take the activated mesoporous silica nanoparticles, add 2mL of 3-aminopropyltriethoxysilane APTES, and place in 20mL of toluene, reflux at 80°C for 12h. After the reaction, the reaction solution was centrifuged, centrifuged and washed three times with absolute ethanol,...

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Abstract

The invention belongs to the field of biological catalysis, and particularly relates to an immobilization method for horse radish peroxidase and application thereof. The carrier used by immobilized horse radish peroxidase is aminated mesoporous silicon dioxide nanoparticles, and the horse radish peroxidase is immobilized by a physical adsorption method. After amination, the amount of the peroxidase adsorbed by the mesoporous silicon dioxide nanoparticles is increased, and thereby the stability of the peroxidase is increased to a certain degree. The immobilized horse radish peroxidase is applied in the treatment of phenol compounds in sewage.

Description

technical field [0001] The invention belongs to the field of biocatalysis, and in particular relates to a peroxidase immobilization method and application thereof. Background technique [0002] As a kind of biocatalyst, enzyme has the characteristics of high catalytic efficiency, high selectivity, and mild reaction conditions, and can be used in various industries. However, the characteristics of natural enzymes such as easy inactivation, poor stability, and low recovery rate limit its various applications. [0003] Immobilized enzyme technology is by physical or chemical means. It is a technology that restricts free enzymes to a certain area for active and unique catalytic action, and can be recycled. Compared with free enzymes, immobilized enzymes have the following advantages: (1) It is easy to separate from the substrate and can be reused; (2) The amount of enzyme used in the enzymatic hydrolysis reaction is reduced, which reduces the cost; (3) Continuous production is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N9/08C02F3/34C02F101/34
CPCC02F3/342C02F2101/345C12N9/0065C12N11/14C12Y111/01007
Inventor 陈宪刘旸李智陈畅鸣
Owner FUZHOU UNIV
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