Transgenic method for increasing plant vegetative tissue oil content, expression vector and application

Abstract

The invention relates to a transgenic method capable of increasing the grease content of nutritive tissue of plants, an expression vector and an application and belongs to the field of genetic engineering. Four genes LEC2, DGAT1, O LEO2 and GAT2 participating different grease synthesis and accumulation processes are selected respectively and linked with proper promoters, multiple monogene / polygene expression vectors are established and are imported into the nutritive tissue of the plants through transfection of agrobacterium tumefaciens, accordingly, ectopic or heterologous expression of the nutritive tissue of plants is realized, and the grease content of the nutritive tissue of plants is increased. Besides, the technology can also be used for increasing production of liposoluble substances related to grease synthesis and accumulation in the nutritive tissue of the plants.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a transgenic method for increasing the oil content of plant vegetative tissues, an expression vector and its application. Background technique [0002] Vegetable oils are versatile and are not only a major source of cooking oil, but are also used in animal feed, soaps, surfactants, cosmetics, paints, lubricants, and in the production of biodiesel. However, due to population growth and reduction of arable land, China will face severe shortages of edible oil and chemical crude oil for a long period of time now and in the future. It is hoped that by improving and changing the agronomic and quality traits of oil plants (including seed yield, oil content and fatty acid composition), plants will not only be the main source of edible oil, but also become a sustainable production of bioenergy and Potential factory for chemical raw materials. [0003] It is gener...

AI Technical Summary

Problems solved by technology

However, based on the limitations of vector tolerance and expression, what kind of gene combination to choose and how to reasonably construct transcription units to achieve gene expression are still technical problems that need to be considered and solved first.

[0006] Saccharomyces cerevisiae contains two 3-phosphoglycerol acyltransferase (GPAT) genes GAT1 and GAT2 encoding the sn-1 position, which catalyze the first acylation reaction of the glyceride biosynthetic pathway. Although GAT1 and GAT2 belong to the GPAT family genes, Moreover, there are conserved acyltransferase characteristic sequences in yeast Gat1p and Gatp2 polypeptides. However, using Saccharomyces cerevisiae as a model organism, by constructing a GAT1 and GAT2 gene knockout or overexpression system, it was found that GAT1 and GAT2 genes have many physiological effects on yeast. Significant differences

In addition, Oleosin protein plays an important role in regulating the stability of liposomes in seeds. At present, there is no research on the combination of Oleosin gene and other genes for co-expression in the vegetative organs of oil crops.

Images