Preparation method of rumen-derived microbial fermentation feed rich in volatile fatty acid calcium

A microbial fermentation and rumen microorganism technology, applied in the field of enzyme preparations, microorganisms, and feed, can solve the problems of irritation, difficult drying, and high drying costs, and achieve the effect of increasing feed intake and promoting digestion.

Inactive Publication Date: 2018-01-05
FLSUGARPEPTIDE BIOLOGY ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of this application is to overcome the shortcomings of the above-mentioned rumen liquid microbial liquid fermentation feed buffer at home and abroad, such as pH regulation, difficulty in drying, and high drying cost, and provide a kind of volatile fatty acid calcium, rumen bacterium protein, active small peptide, oligosaccharide, Nutrients such as probiotics, rumen-derived microbial fermented feed with the functions of stimulating lactation, promoting digestion and increasing feed intake

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  • Preparation method of rumen-derived microbial fermentation feed rich in volatile fatty acid calcium
  • Preparation method of rumen-derived microbial fermentation feed rich in volatile fatty acid calcium

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Embodiment 1

[0059] A method for preparing rumen-derived microbial fermented feed rich in volatile fatty acid calcium, comprising the following steps:

[0060] ①. Preparation of rumen microbial strain liquid:

[0061] Gather 20kg (195L) of the rumen liquid of 5 healthy dairy cows to obtain 200kg of the rumen microbial strain liquid;

[0062] ②. Preparation of rumen microbial fermentation liquid:

[0063] Grind 50kg of soybean meal, 150kg of corn and 400kg of wheat straw, add 1400kg of water to adjust the slurry, and then pump it into the fermenter; add steam to raise the temperature to 39°C and continue to pass in CO for 20 minutes 2 After excluding the oxygen in the feed slurry, add 20kg of trace element nutrient buffer and 200kg of rumen microbial strain solution, and strictly anaerobic fermentation under the condition of continuous flow of calcium hydroxide or calcium carbonate fine powder for 72 hours, to obtain 2200kg of rumen microbial fermentation broth.

[0064] ③. Preparation o...

Embodiment 2

[0075] A method for preparing rumen-derived microbial fermented feed rich in volatile fatty acid calcium, comprising the following steps:

[0076] ①. Preparation of rumen microbial strain liquid:

[0077] Gather 5 kg (4.9 L) of the rumen liquid of 5 healthy goats to obtain 50 kg of the rumen microbial strain liquid;

[0078] ②. Preparation of rumen microbial fermentation liquid:

[0079] Grind 10kg of soybean meal, 50kg of corn and 100kg of wheat straw, add 340kg of water to adjust the slurry, and then pump it into the fermenter; add steam to raise the temperature to 39°C and continue to pass in CO for 10 minutes 2 After excluding the oxygen in the feed slurry, add 3kg of trace element nutrient buffer and 50kg of rumen microbial seed liquid, and strictly anaerobically ferment for 96 hours under the condition of continuous flow of calcium hydroxide or calcium carbonate fine powder, and obtain 550kg of rumen microbial fermentation broth.

[0080] ③. Preparation of rumen micro...

Embodiment 3

[0091] ①. Preparation of rumen microbial strain liquid:

[0092] Gather 30kg (195L) of the rumen liquid of 5 healthy beef cattle to obtain 300kg of the rumen microbial strain liquid;

[0093] ②. Preparation of rumen microbial fermentation liquid:

[0094] Grind 100kg cottonseed meal, 100kg corn, 100kg barley, 700kg wheat straw, add 2500kg water to adjust the slurry, and then pump it into the fermenter; add steam to raise the temperature to 39°C and continue to pass in CO for 20 minutes 2 After excluding the oxygen in the feed slurry, add 50kg of trace element nutrient buffer and 300kg of rumen microbial strain solution, and strictly anaerobic fermentation under the condition of continuous flow of calcium hydroxide or calcium carbonate fine powder for 72 hours, to obtain 4300kg of rumen microbial fermentation broth.

[0095] ③. Preparation of rumen microbial fermentation broth solids wet base and liquid nutrient solution:

[0096] Through solid-liquid separation equipment, 4...

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Abstract

The invention relates to a preparation method of a rumen-derived microbial fermentation feed rich in volatile fatty acid calcium. The preparation method comprises the following steps of after collecting rumen liquor, performing direct culture or in vitro culture so as to prepare strain liquor; crushing fine and coarse feed products, performing pulping to obtain pulp, and pumping the pulp into a fermenter; after raising the temperature and introducing CO2 for deoxygenization, adding trace element nutrient buffering liquor and the strain liquor, strictly performing anaerobic fermentation to prepare fermentation liquor, then performing solid-liquid separation to obtain a solid wet base and liquid nutrient liquor; and directly concentrating the liquid nutrient liquor to prepare concentrated liquid nutrient liquor; mixing the fermentation liquor solid wet base with feed raw materials, pure water, compound strain liquor and compound enzymes, and performing enzymolysis fermentation, so as toprepare fermented and enzymatically hydrolysed solid wet bases; mixing the fermented and enzymatically hydrolysed solid wet bases with the concentrated nutrient liquor to prepare the fermentation feedrich in volatile fatty acid calcium. The prepared feed is rich in nutrients of the volatile fatty acid calcium, mycoprotein, active small peptides, oligosaccharides, probiotics and the like, and hasthe efficacy of stimulating lactation, promoting digestion, increasing food consumption and the like.

Description

technical field [0001] The application belongs to the field of feed, enzyme preparation and microorganism technology, and in particular relates to a method for preparing rumen-derived microbial fermented feed rich in volatile fatty acid calcium. Background technique [0002] Rumen microbial fermentation and its nutrition have been a hot research topic in ruminant nutrition for many years. The rumen is the first stomach of ruminants near the esophagus, with a volume of about 70-120 liters. It can be regarded as a continuous inoculated active fermenter for the reproduction of anaerobic microorganisms. In a sense, people feeding ruminants are actually feeding the microbes in their rumens. The research found that: rumen microorganisms mainly include bacteria, fungi and rumen protozoa. Bacteria include: fiber-degrading bacteria, hemi-fiber-degrading bacteria, starch-degrading bacteria, protein-degrading bacteria, fat-degrading bacteria, acid-utilizing bacteria, lactic acid-prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/10A23K10/12A23K10/18A23K10/14
Inventor 程勐万里刘萱仪孟龙
Owner FLSUGARPEPTIDE BIOLOGY ENG
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