A simple and rapid method for the determination of six fatty acid ethyl esters in human whole blood

Abstract

The invention relates to a method for simple and rapid determination of six fatty acid ethyl esters in human whole blood, belonging to the technical fields of drug detection and forensic identification, wherein the six fatty acid ethyl esters include ethyl myristate, ethyl palmitate, ethyl stearate, ethyl oleate, ethyl linoleate and ethyl arachidonate. The method is low in the usage amount of samples, and as few as 200 [mu]L of samples are examined. The process of pretreatment is simple and fast. A HP-5MS capillary gas chromatographic column and a mass spectrometric detector are employed for determination of the contents of the six fatty acid ethyl esters in human whole blood. The method has the advantages of high sensitivity, good specificity, a wide linear range, simple and fast operation and low usage amount of extraction solvents, and is capable of satisfying high requirements of urgent forensic identification tasks on detection time and easy to operate and promote.

Description

technical field [0001] The invention belongs to the technical field of drug detection and forensic identification, and relates to, in particular, a simple and rapid determination of six fatty acid ethyl esters (ethyl myristate, ethyl palmitate, ethyl stearate, ethyl oleate) in human whole blood. ester, ethyl linoleate, ethyl arachidonic acid) method. Background technique [0002] The data show that the abuse of ethanol is very common in today's society and has produced many adverse effects on society. In forensic work, the detection of ethanol is of great significance for the cause investigation and responsibility determination of traffic accidents and other cases. However, in actual cases, due to the rapid metabolism of ethanol, and sometimes ethanol may be produced during the process of corpse decomposition, the detection of ethanol in the sample cannot truly reflect the drinking situation. Studies have shown that fatty acid ethyl esters include more than 20 compounds, a...

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Problems solved by technology

[0003] The currently disclosed methods for the determination of fatty acid ethyl esters in biological samples only detect hair, meconium, and serum, and there are problems such as cumbersome sample pretreatment steps and a large amount of sample materials.

Whole blood is the most important biological sample in forensic poison identification, especially for cases involving death, due to the occurrence of hemolysis after death, often only whole blood samples can be obtained, but plasma or serum biological samples cannot be obtained. So far, there is no report on the analytical method using whole blood as the detection object

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