Method for simultaneously detecting nine types of oestrogen substances in water by solid phase extraction-liquid chromatography-tandem mass spectrometry

Abstract

The invention discloses a method for simultaneously detecting nine types of oestrogen substances in water by solid phase extraction-liquid chromatography-tandem mass spectrometry. The method comprises(1) carrying out water sample pretreatment, (2) enriching and purifying a target oestrogen with a solid phase extraction small column and (3) quantificationally detecting nine types of oestrogen substances in water through an internal standard method through a high efficiency liquid chromatography-tandem mass spectrometer. The method utilizes a single factor test, optimizes the type of a solid phase extraction small column, pH in solid phase extraction, the type and use amount of an eluting agent and the type and use amount of an eluent, optimizes a liquid chromatographic column temperature,a flowing rate, a sample loading amount, a flowing amount, the fluid type and a gradient ratio, has short peak production time and produces an ion chromatogram having a good separation degree. The method utilizes the internal standard substance indicating a recovery rate before solid phase extraction, well shows the loss of the target substances in the pretreatment and produces a real and reliablefinal result.

Description

Technical field [0001] The invention belongs to the technical field of safety detection of trace organic pollutant residues in water environments, relates to pretreatment methods and instrument detection and analysis techniques for 9 conventional estrogen substances in three water environments, and specifically relates to a solid phase extraction-liquid chromatography Tandem mass spectrometry is a method for the simultaneous detection of 9 estrogen substances in water. technical background [0002] In recent years, environmental endocrine disruptors (EDCs) have attracted widespread attention. Studies have shown that EDCs can induce feminization of male fish, affect the growth and reproduction of aquatic organisms, and increase the incidence of human breast cancer. Natural and synthetic estrogens are the most important EDCs. They have the strongest endocrine disrupting properties, and the nanogram level is sufficient to cause adverse effects on aquatic organisms. Natural estrogen...

AI Technical Summary

Problems solved by technology

However, when the existing solid-phase extraction combined with liquid chromatography-mass spectrometry is used for the detection of estrogens in the water environment, most of them are due to poor pretreatment, or the pretreatment conditions of solid phase extraction, liquid chromatography conditions and / or mass spectrometry The conditions are not well selected, and there are more or less problems such as inaccurate test results, instability, excessive interference, large consumption of organic reagents, limited quantity and range of estrogen detection, etc.

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