Method for simultaneously detecting nine types of oestrogen substances in water by solid phase extraction-liquid chromatography-tandem mass spectrometry

A technology of liquid chromatography and tandem mass spectrometry, which is applied in the field of simultaneous detection of 9 estrogen substances in water by solid phase extraction-liquid chromatography tandem mass spectrometry, can solve the problem of limited quantity and range of estrogen detection, inaccurate detection results, Problems such as large consumption of organic reagents can achieve the effects of saving manpower and material costs, less time consumption and high recovery rate
CN107543876AInactive Publication Date: 2018-01-05SHANGHAI ACADEMY OF ENVIRONMENTAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI ACADEMY OF ENVIRONMENTAL SCIENCES
Publication Date
2018-01-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for simultaneously detecting nine types of oestrogen substances in water by solid phase extraction-liquid chromatography-tandem mass spectrometry. The method comprises(1) carrying out water sample pretreatment, (2) enriching and purifying a target oestrogen with a solid phase extraction small column and (3) quantificationally detecting nine types of oestrogen substances in water through an internal standard method through a high efficiency liquid chromatography-tandem mass spectrometer. The method utilizes a single factor test, optimizes the type of a solid phase extraction small column, pH in solid phase extraction, the type and use amount of an eluting agent and the type and use amount of an eluent, optimizes a liquid chromatographic column temperature,a flowing rate, a sample loading amount, a flowing amount, the fluid type and a gradient ratio, has short peak production time and produces an ion chromatogram having a good separation degree. The method utilizes the internal standard substance indicating a recovery rate before solid phase extraction, well shows the loss of the target substances in the pretreatment and produces a real and reliablefinal result.
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Description

Technical field

[0001] The invention belongs to the technical field of safety detection of trace organic pollutant residues in water environments, relates to pretreatment methods and instrument detection and analysis techniques for 9 conventional estrogen substances in three water environments, and specifically relates to a solid phase extraction-liquid chromatography Tandem mass spectrometry is a method for the simultaneous detection of 9 estrogen substances in water. technical background

[0002] In recent years, environmental endocrine disruptors (EDCs) have attracted widespread attention. Studies have shown that EDCs can induce feminization of male fish, affect the growth and reproduction of aquatic organisms, and increase the incidence of human breast cancer. Natural and synthetic estrogens are the most important EDCs. They have the strongest endocrine disrupting properties, and the nanogram level is sufficient to cause adverse effects on aquatic organisms. Natural estrogen...

Claims

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