A method for the simultaneous enrichment and detection of phenolic, estrogenic and androgenic endocrine disruptors in drinking water

A technology for endocrine disruptors and estrogen, which is applied in the field of estrogen and androgen endocrine disruptors, and enriches and detects phenols in drinking water at the same time, achieving the effects of saving sampling time, high sensitivity, and small relative standard deviation

Active Publication Date: 2019-08-23
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, in addition to phenolic and estrogenic EDCs, there are many kinds of androgenic EDCs in the water environment, such as TREN, TES, Me-TES, etc., but so far there is no one that can detect these three at the same time. The analysis and detection method of EDCs, the method of the present invention just fills this gap, and can realize the one-time quantitative analysis of the concentration of 11 kinds of EDCs in three categories: phenols, estrogen and androgen in drinking water, which greatly improves the detection efficiency

Method used

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  • A method for the simultaneous enrichment and detection of phenolic, estrogenic and androgenic endocrine disruptors in drinking water
  • A method for the simultaneous enrichment and detection of phenolic, estrogenic and androgenic endocrine disruptors in drinking water
  • A method for the simultaneous enrichment and detection of phenolic, estrogenic and androgenic endocrine disruptors in drinking water

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Embodiment 1

[0077] A method for simultaneously enriching and detecting phenols, estrogens, and androgens endocrine disruptors in drinking water, the specific steps for making a standard working curve are as follows:

[0078] (a) Prepare a mixed standard stock solution: use 50% methanol / water solution (v / v) to mix the three types of EDCs with known concentrations to prepare a mixed standard stock solution with the same concentration of each EDCs, and place it in a brown reagent bottle;

[0079] (b) Dilute the mixed standard stock solution in step (a) with 50% methanol / water solution (v / v) to different concentration gradients: 1 μg / L, 5 μg / L, 10 μg / L, 20 μg / L, 50 μg / L, 100μg / L, 200μg / L, 500μg / L and 1000μg / L, at the same time, add 200μg / L BPA-D16, 200μg / L E2-D2 and 100μg / L TES-D3 three internal standards to the samples of each concentration gradient;

[0080] (c) LC-MS / MS is used to analyze each concentration gradient sample, and the above-mentioned optimized liquid chromatography operating ...

Embodiment 2

[0087] A method for simultaneously enriching and detecting phenols, estrogens and androgenic endocrine disruptors in drinking water, comprising the following steps:

[0088] (1) Sample pretreatment:

[0089] Select a water sample from a water source in East China, use a 0.7 μm glass fiber membrane as a filter to remove suspended solids, adjust the pH to 5.0 and add complexing agent Na 2 EDTA, and then add three types of internal standards with known amounts: 200 μg / L of BPA-D16, 200 μg / L of E2-D2 and 100 μg / L of TES-D3 to complete the pretreatment;

[0090] (2) Concentration and enrichment of target EDCs:

[0091] First use 5-10mL equal volume of methanol, ultrapure water and dilute hydrochloric acid solution to pass through the SPE cartridge (Oasis HLB solid phase extraction cartridge) sequentially for activation, and then take 500mL of the water sample pretreated in step (1) and extract it through the column Enrichment, then take 5% methanol / water solution (v / v) as eluent ...

Embodiment 3

[0100] A method for simultaneously enriching and detecting phenols, estrogens and androgenic endocrine disruptors in drinking water, comprising the following steps:

[0101] (1) Sample pretreatment:

[0102] Select a water sample from a water source in East China, use a 0.7 μm glass fiber membrane as a filter to remove suspended solids, adjust the pH to 5.0 and add complexing agent Na 2 EDTA, and then add three types of internal standards with known amounts: 200 μg / L of BPA-D16, 200 μg / L of E2-D2 and 100 μg / L of TES-D3 to complete the pretreatment;

[0103] (2) Concentration and enrichment of target EDCs:

[0104] First use 5-10mL equal volume of methanol, ultrapure water and dilute hydrochloric acid solution to pass through the SPE cartridge (Oasis HLB solid phase extraction cartridge) sequentially for activation, and then take 500mL of the water sample pretreated in step (1) and extract it through the column Enrichment, then take 5% methanol / water solution (v / v) as eluent ...

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Abstract

The invention relates to a method for simultaneously enriching and detecting phenolic, estrogenic and androgenic EDCs (Endocrine Disrupting Chemicals) in drinking water. The method comprises the following steps: firstly, concentrating and enriching a pretreated water sample by adopting an SPE (Solid Phase Extraction) method; secondly, analyzing and detecting by utilizing LC (Liquid Chromatography)-MS (Mass Spectrometry) / MS; finally, determining the concentration of three kinds of EDCs in a water sample according to an analysis chromatogram of the sample and a standard working curve of the three kinds of EDCs. Compared with the prior art, simultaneous enrichment and detection on trace (micro) typical phenolic, estrogenic and androgenic EDCs in the drinking water is realized, the complexity of independently analyzing and detecting different kinds of EDCs is avoided, the sample detecting time is greatly saved, and the detection cost is reduced; meanwhile, the accuracy of a detection result is reliable, the detection limit is low, the relative standard deviation is small, and the like.

Description

technical field [0001] The invention relates to a detection and analysis technology for trace (micro) organic pollutants in the water environment, in particular to a method for simultaneously enriching and detecting phenols, estrogens and androgen endocrine disruptors in drinking water. Background technique [0002] Endocrine Disrupting Chemicals (EDCs), also known as environmental hormones, is an exogenous chemical substance that interferes with the endocrine system. Its presence in the environment can interfere with various aspects of the human or animal endocrine system and cause abnormal effects . Although EDCs do not directly cause abnormal effects on organisms as toxic substances, they can affect organisms through various channels such as ingestion and accumulation. Phenomena, including causing animal and human genital disorders, abnormal behavior, decreased reproductive ability, larval death, and even the extinction of species in severe cases. At present, the analyt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/08G01N30/88
Inventor 崔长征姜蕾张天阳张东薛佳怡金磊曹赞
Owner EAST CHINA UNIV OF SCI & TECH
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