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dna barcodes, primer pairs, kits and methods for identifying shrew species

A barcoding, shrew technology, applied in the field of kits, primer pairs, and DNA barcoding to identify shrew species, can solve the problems of high classification personnel and identification experience, inconvenient comparative research, etc., to achieve professional level and laboratory. Effects with low requirements

Active Publication Date: 2019-01-29
MUDANJIANG NORMAL UNIV
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Problems solved by technology

However, the appearance of Shrew species is relatively similar, and none of the holotype specimens of Shrew species distributed in my country used for morphological classification were collected by Chinese researchers, and these holotype specimens are all deposited in foreign countries. In herbariums and museums, comparative research is very inconvenient, so morphological classification requires classification personnel and identification experience

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  • dna barcodes, primer pairs, kits and methods for identifying shrew species
  • dna barcodes, primer pairs, kits and methods for identifying shrew species
  • dna barcodes, primer pairs, kits and methods for identifying shrew species

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Embodiment

[0047] The technical scheme of the present invention is as follows:

[0048] First, extract the total DNA from the collected Shrew species tissues, use a pair of primers to amplify the Cyt b gene fragments, sequence the amplified products, and then perform sequence analysis and comparison to establish the sequences of various Shrew species The standard database, on the basis of comparing the DNA of the database, through the analysis of the polymerase chain reaction product results under specific conditions, so as to achieve the purpose of rapid and accurate identification of Shrew species. Extract the total DNA of the shrew sample that needs to be identified, and use the designed specific primers for PCR amplification under given conditions, confirm the PCR amplification product result by agarose gel electrophoresis, and then sequence and compare the sequence according to the sequence. The shrew species can be identified. The molecular identification method for identifying Shrew...

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Abstract

The invention relates to the technical field of molecular identification and particularly relates to a DNA bar code, a primer pair, a kit and a method for identifying shrew species. The method comprises the following steps: amplifying the Cytb gene segment of a shrew sample to be detected and sequencing; comparing sequences according to 14 DNA bar codes provided by the invention, and determining that the shrew sample to be detected and the DNA bar codes belong to the same shrew species if the homology between the Cytb gene segment of the shrew sample to be detected and any one of the DNA bar codes is 96% or above. The DNA bar code, the primer pair, the kit and the method are capable of rapidly and accurately identifying 14 species of the shrews in China and reducing the identification time.

Description

Technical field [0001] The present invention relates to the technical field of molecular identification, and in particular to a DNA barcode, primer pair, kit and method for identifying Shrew species. Background technique [0002] The genus Sorex belongs to the order Soricomorpha (Soricidae) and the subfamily Shrew (Soricinae). There are 16-18 species distributed in my country. Shrews are primitive mammals with long snouts, small eyes and mouse-like shapes. They are closely related to moles and hedgehogs. Some species of Shrew are carriers of epidemic hemorrhagic fever virus. Different species carry different epidemic hemorrhagic fever viruses. Close contact with people in the external environment. The identification of its species is the key to preventing and controlling epidemic hemorrhagic fever. However, the appearances of Shrew species are relatively similar, and none of the orthomorph specimens of the Shrew species distributed in my country for morphological classificatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 刘铸张隽晟蔡赫
Owner MUDANJIANG NORMAL UNIV
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