A kind of in vitro maturation culture medium of oocytes added with rosmarinic acid and its application

Abstract

The invention relates to the technical field of cell culture and in particular relates to an oocyte in-vitro maturation culture solution added with rosmarinic acid and application thereof. The in-vitro maturation culture solution is characterized by being prepared from a component rosmarinic acid; the concentration range of the rosmarinic acid is 2muM to 200muM. According to the oocyte in-vitro maturation culture solution provided by the invention, the rosmarinic acid is added into a pig oocyte maturation culture solution and is matched with the synergistic effect of other components, so thatthe maturation quality of pig oocytes is improved and the oocyte in-vitro maturation culture solution has a good promotion effect on pig embryo development. A result shows that the in-vitro maturationculture solution provided by the invention can be used for improving the anti-oxidization capability of the oocytes and the rosmarinic acid with the suitable concentration can be used for effectivelyimproving the oocyte maturation quality, so that the in-vitro development potential of the pig oocytes is improved. The in-vitro maturation culture solution is simple to prepare and convenient to operate and has a relatively great application prospect.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an oocyte in vitro maturation culture solution added with rosmarinic acid and its application. Background technique [0002] In vitro maturation of mammalian oocytes is key to the in vitro production of animal embryos and precursors to embryonic engineering such as transgenic cloning. In the in vitro microenvironment of oocyte maturation, if some reactive oxygen species produced by oxygen metabolism are not removed in time, oxidative stress will be induced. Studies have shown that oxidative stress is one of the main factors affecting the quality of oocytes and the developmental potential of embryos. A large amount of oxidative stress can lead to changes in the morphology and function of mitochondria in oocytes, thereby affecting the synthesis of adenosine triphosphate (ATP), interfering with Meiosis in oocytes increases the number of aneuploid chromosomes in oocytes, result...

AI Technical Summary

Problems solved by technology

Studies have shown that oxidative stress is one of the main factors affecting the quality of oocytes and the developmental potential of embryos. A large amount of oxidative stress can lead to changes in the morphology and function of mitochondria in oocytes, thereby affecting the synthesis of adenosine triphosphate (ATP), interfering with Meiosis in the oocyte increases the number of aneuploid chromosomes in the oocyte, causing DNA damage and early developmental arrest in the embryo, ultimately leading to adverse pregnancy outcomes

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