Molecular markers capable of predicting and identifying sheep wool length and their specific primer pairs and applications
A molecular marker and specific technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of cumbersome steps in wool length detection method, increase the cost of breeding, and the accuracy needs to be improved and other problems, to achieve the effect of speeding up the genetic selection process, high accuracy and simple operation.
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Embodiment 1
[0021] Example 1: The molecular marker capable of predicting and identifying sheep wool length is located at exon 1 of chromosome 1 (120571805) of IFNAR2 gene.
Embodiment 2
[0022] Example 2: The pair of specific primers used to amplify the molecular markers described in the above examples that can predict and identify sheep wool length includes two allele-specific primers (ASP1 / ASP2), one locus-specific Sex primer (LSP) and a specific targeting amplification primer (STA), the sequence of an allele-specific primer (ASP1) is shown in the sequence table SEQ ID No.2, another allele-specific primer ( The sequence of ASP2) is shown in the sequence table SEQ ID No.3, the sequence of the locus-specific primer is shown in the sequence table SEQ ID No.4, and the sequence of the specific targeting amplification primer is shown in the sequence table SEQ ID No.5 shown.
Embodiment 3
[0023] Example 3: The application of the specific primer pair described in Example 2 as a preparation or a kit for preparing a molecular marker capable of predicting and identifying sheep wool length in vitro.
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