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Molecular markers capable of predicting and identifying sheep wool length and their specific primer pairs and applications

A molecular marker and specific technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of cumbersome steps in wool length detection method, increase the cost of breeding, and the accuracy needs to be improved and other problems, to achieve the effect of speeding up the genetic selection process, high accuracy and simple operation.

Active Publication Date: 2021-03-05
新疆畜牧科学院畜牧研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, when breeding sheep breeds with excellent wool length properties, when it is necessary to measure the wool length of sheep that have grown for more than several months, the sheep breeds are selected according to the length measurement results. As can be seen from the above, the steps of the wool length detection method It is cumbersome, the detection efficiency is low, and the accuracy needs to be improved, and the detection objects are sheep that have grown for more than several months, which increases the cost of breeding, including time cost and other breeding costs

Method used

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  • Molecular markers capable of predicting and identifying sheep wool length and their specific primer pairs and applications
  • Molecular markers capable of predicting and identifying sheep wool length and their specific primer pairs and applications
  • Molecular markers capable of predicting and identifying sheep wool length and their specific primer pairs and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: The molecular marker capable of predicting and identifying sheep wool length is located at exon 1 of chromosome 1 (120571805) of IFNAR2 gene.

Embodiment 2

[0022] Example 2: The pair of specific primers used to amplify the molecular markers described in the above examples that can predict and identify sheep wool length includes two allele-specific primers (ASP1 / ASP2), one locus-specific Sex primer (LSP) and a specific targeting amplification primer (STA), the sequence of an allele-specific primer (ASP1) is shown in the sequence table SEQ ID No.2, another allele-specific primer ( The sequence of ASP2) is shown in the sequence table SEQ ID No.3, the sequence of the locus-specific primer is shown in the sequence table SEQ ID No.4, and the sequence of the specific targeting amplification primer is shown in the sequence table SEQ ID No.5 shown.

Embodiment 3

[0023] Example 3: The application of the specific primer pair described in Example 2 as a preparation or a kit for preparing a molecular marker capable of predicting and identifying sheep wool length in vitro.

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Abstract

The invention relates to the technical field of animal molecular markers, and relates to a molecular marker capable of predicting and identifying sheep wool length and the application of specific primer pairs in predicting and identifying the wool length traits of Chinese Merino sheep, which can predict and identify sheep wool length. A molecular marker of wool length located at exon 1 of chromosome 1 of the IFNAR2 gene. The present invention discloses for the first time the use of the rs407032027 SNP site as a molecular marker capable of predicting and identifying sheep wool length, and the application of the rs407032027 SNP site to the selection of the wool length traits of Chinese Merino sheep; Molecular markers of wool length have the advantages of simple operation, low cost, high accuracy, and automatic detection for Chinese Merino sheep (Xinjiang type) when selecting sheep breeds with high-quality wool length traits.

Description

technical field [0001] The invention relates to the technical field of animal molecular markers, and relates to a molecular marker capable of predicting and identifying the length of sheep wool and a pair of specific primers. The application in the preparation or kit, the application of the molecular marker in predicting and identifying the wool length traits of Chinese Merino sheep. Background technique [0002] As the wool spinning industry at home and abroad continues to update wool and fabric processing, the requirements for raw material wool are more stringent, and the product trend has been developing in the direction of lightness and softness. There have also been new milestones in the quality of wool in the world. The demand for wool in the wool spinning industry There is a gradual increasing trend. Wool length is one of the key physical indicators to measure the quality of wool. At present, one of the main problems facing the production of fine-wool sheep in our c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 田可川黄锡霞赵冰茹付雪峰徐新明田月珍吴伟伟石刚哈尼克孜于丽娟张艳花马桢朱桦陈华峰玛尔孜娅拉扎提
Owner 新疆畜牧科学院畜牧研究所
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