Method for using semi-continuous method to produce polymalic acid with different molecular weights

A polymalic acid and molecular weight technology, which is applied in the field of microbial fermentation, can solve the problems of difficult degradation of artificially synthesized polymer materials, and achieve the effects of good fermentation stability, short fermentation time and high repetition rate.

Inactive Publication Date: 2018-02-02
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While its importance is becoming more and more significant, its shortcomings also appear

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1

[0020] A semi-continuous method for producing polymalic acid with different molecular weights includes the following steps:

[0021] (1) Seed cultivation

[0022] The Aureobasidium pullulans strain was activated for 2 hours, and the spores were washed with sterile normal saline to prepare a spore suspension. According to the 10% inoculum amount, it was received into a 500ml baffle bottle containing 100ml seed culture medium, the culture temperature was 25℃, the shaking speed was 200r / min, and the culture time was 40h.

[0023] (2) Fermentation culture

[0024] The inoculation port is protected by flame, and the obtained seed liquid is connected to a 5L fermentor with 3L fermented liquid in a quantity of 10% (v / v) for fermentation culture; rotation speed is 500r / min, ventilation volume is 2.5V·V·m , The tank pressure is 0.1Mpa and the temperature is 25 ℃. The components of the fermentation medium are: sucrose 180g / L, peptone 35g / L, KH 2 PO 4 0.1g / L, NaNO 3 2g / L, M...

Example Embodiment

[0027] Example 2

[0028] (1) Seed cultivation

[0029] The same as in Example 1.

[0030] (2) Fermentation culture

[0031] The inoculum is protected by a flame, and the obtained seed liquid is connected to a 5L fermentor containing 3L of fermentation broth in an amount of 10% (v / v) for fermentation culture. The speed is 500r / min, the ventilation is 2.5V·V·m, the tank pressure is 0.1Mpa, and the temperature is 25 ℃. The components of the fermentation medium are: sucrose 180g / L, peptone 35g / L, KH 2 PO 4 0.1g / L, NaNO 3 2g / L, MgSO 4 ·7H 2 O 0.3g / L, KCl 0.5g / L, MnSO 4 0.05g / L, the balance is water.

[0032] After fermentation to 108h, 45% of the fermentation broth was released, which was recorded as the "first batch", and then the same volume of fermentation medium was added to it, and the pH was adjusted to 6.5. Continue to ferment for 98 hours and then release 50% of the fermentation broth. This batch of fermentation broth is recorded as the "second batch". After that, continue to...

Example Embodiment

[0034] Example 3

[0035] (1) Seed cultivation

[0036] The same as in Example 1.

[0037] (2) Fermentation culture

[0038] The inoculum is protected by a flame, and the obtained seed liquid is connected to a 5L fermentor containing 3L of fermentation broth in an amount of 10% (v / v) for fermentation culture. The speed is 500r / min, the ventilation is 2.5V·V·m, the tank pressure is 0.1Mpa, and the temperature is 25 ℃. The components of the fermentation medium are: sucrose 180g / L, peptone 35g / L, KH 2 PO 4 0.1g / L, NaNO 3 2g / L, MgSO 4 ·7H 2 O 0.3g / L, KCl 0.5g / L, MnSO 4 0.05g / L, the balance is water.

[0039] After 90 hours of fermentation, 45% of the fermentation broth was released, which was recorded as the "first batch", and then the same volume of fermentation medium was added to it, and the pH was adjusted to 7.0. Continue to ferment for 100h and then release 50% fermentation broth. This batch of fermentation broth is recorded as the "second batch". Then continue to supplement th...

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Abstract

The invention relates to a method for using a semi-continuous method to produce polymalic acid with different molecular weights. The method comprises the following steps of preparing a seed solution,wherein original bacterial strains of the aureobasidium pullulans are inoculated to a slant culture medium to be cultured and activated; inoculating the activated slant seeds to a seed culture mediumto obtain a seed solution for producing polymalic acid; inoculating the seed solution to a fermentation culture medium to be fermented; after fermentation is finished, conducting centrifugation to remove thallus, and conducting alcohol precipitation to separate out polymalic acid. By changing an inherent fermentation mode, adopting different time points to drain out fermentation liquid, supplementing the fermentation culture medium with the same size, and adjusting the fermentation pH, the polymalic acid molecular weights obtained at different time points are different, and the purpose of obtaining polymalic acid with different molecular weights is achieved. Compared with the method of conducting fermentation in batches, and obtaining polymalic acid with different molecular weights in batches, the fermentation time needed for semicontinuous fermentation is shorter, and the fermentation stability is higher.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and relates to a semi-continuous method for producing polymalic acid with different molecular weights. Background technique [0002] Polymer materials refer to materials based on polymer compounds, which are composed of substances with relatively high molecular weights. With the development of industrialization, polymer materials have been widely concerned in science, medicine and industry. While its importance is becoming more and more significant, its shortcomings also appear in front of people. Most of the artificially synthesized polymer materials are difficult to degrade in nature. In today's deteriorating environment, people are beginning to pay attention to the environmental pollution caused by polymer materials that cannot be decomposed. With the increasing awareness of environmental protection, people gradually increase the requirements for the degradability of materials...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12R1/645
CPCC12P7/625
Inventor 乔长晟赵廷彬李雪张卫
Owner 天津北洋百川生物技术有限公司
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