Sample target for mass spectrum analysis, preparation method thereof, and mass spectrum analysis method

Abstract

The invention discloses a sample target for mass spectrum analysis, a preparation method thereof, and a mass spectrum analysis method. The mass spectrum analysis method comprises the following steps of first, sticking and fixing polyimide on a substrate; second, irradiating the polyimide with a laser with a power of 2.4-5.6W and a scanning rate of 80mm / s-200mm / s, so as to form graphene oxide witha multi-layer and porous micro-shape on the irradiating point; third, spotting a to-be-detected sample on the graphene oxide directly; and fourth, putting the substrate with the to-be-tested sample into a mass spectrometer, irradiating the graphene oxide and the to-be-tested sample with laser so as to form cocrystallization, and performing mass spectrum analysis. The mass spectrum analysis methodhas low interference in a low quality area, improves the flexibility of the substrate in helping laser desorb mass spectrum, and reduces analysis time and analysis cost.

Description

【Technical field】 [0001] The invention relates to the technical field of detection and analysis, in particular to a sample target for mass spectrometry analysis, a preparation method thereof, and a mass spectrometry analysis method. 【Background technique】 [0002] In mass spectrometry detection, for organic molecules, matrix-assisted laser desorption ionization (MALDI) is usually used to ionize samples for mass spectrometry analysis. [0003] In the process of mass spectrometry, it is generally necessary to perform a large number of pretreatment processes on the sample, including concentration, desalination, etc., and then mix the sample solution with the matrix solution and apply the sample on the target point of the sample. The existing mass spectrometry method involves a large number of pretreatment processes, which makes the efficiency of the overall analysis method low. Moreover, in the existing analysis process, a large number of fragment ions will be generated in the...

AI Technical Summary

Problems solved by technology

Existing mass spectrometry analysis methods involve a large number of pretreatment processes, making the efficiency of the overall analysis method low

Moreover, in the existing analysis process, a large number of fragment ions will be generated in the low-mass region (m / z<500), which seriously interferes with the determination of small molecular substances

Therefore, the application of MALDI mass spectrometry, a high-throughput, high-sensitivity mass spectrometry method, in many important fields such as bioactive small molecule detection, metabolomics analysis, and small molecule mass spectrometry imaging is limited.

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