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Small molecule peptide extracted from giant salamander meat and bone and extraction method thereof

A small molecule peptide and extraction method technology, which is applied in the field of biological peptide separation, can solve the problems of difficult use of small molecule peptide activity, single extraction method, and limited application occasions, and achieve the effects of reduced dosage, strong operability, and reduced pollution

Active Publication Date: 2018-02-09
湖北世厚耀榜纪田农业发展有限公司 +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the existing small molecule peptide extraction technology, the extraction method is single, the extraction efficiency is not high, and the amount of enzyme preparation is relatively high, the applicable occasions are limited, and the activity of the prepared small molecule peptide is difficult to be used in peptide drugs and peptide health products

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  • Small molecule peptide extracted from giant salamander meat and bone and extraction method thereof

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Embodiment 1

[0039] Embodiment 1 of the present invention provides a method for extracting small molecule peptides from giant salamander meat and bones, which is realized through the following steps:

[0040] Step 1, cleaning and pulverizing the giant salamander to obtain giant salamander meat and bones, and cooking the giant salamander meat and bones to obtain giant salamander bone broth;

[0041] Specifically: remove the viscera of the giant salamander, then use a pulverizer to crush the giant salamander, and obtain the giant salamander meat and bone after cleaning, add the giant salamander meat and bone and pure water into the cooking tank, the purified water is twice the weight of the giant salamander meat and bone, Under the pressure of 0.2 MPa, cook at 132°C for 60 minutes under high pressure, discharge the residue, and obtain giant salamander bone broth;

[0042] Step 2, magnetizing the giant salamander bone broth, and then adding the first enzyme preparation, the second enzyme prep...

Embodiment 2

[0052] Embodiment 2 of the present invention provides a method for extracting small molecule peptides from giant salamander meat and bones, which is realized through the following steps:

[0053] Step 1, cleaning and pulverizing the giant salamander to obtain giant salamander meat and bones, and cooking the giant salamander meat and bones to obtain giant salamander bone broth;

[0054] Specifically: remove the viscera of the giant salamander, then use a pulverizer to crush the giant salamander, and obtain the giant salamander meat and bone after cleaning, add the giant salamander meat and bone and pure water into the cooking tank, the purified water is twice the weight of the giant salamander meat and bone, Under the pressure of 0.2 MPa, cook at 132°C for 60 minutes under high pressure, discharge the residue, and obtain giant salamander bone broth;

[0055] Step 2, magnetizing the giant salamander bone broth, and then adding the first enzyme preparation, the second enzyme prep...

Embodiment 3

[0065] Embodiment 3 of the present invention provides a method for extracting small molecule peptides from giant salamander meat and bones, which is realized through the following steps:

[0066] Step 1, cleaning and pulverizing the giant salamander to obtain giant salamander meat and bones, and cooking the giant salamander meat and bones to obtain giant salamander bone broth;

[0067] Specifically: remove the viscera of the giant salamander, then use a pulverizer to crush the giant salamander, and obtain the giant salamander meat and bone after cleaning, add the giant salamander meat and bone and pure water into the cooking tank, the purified water is twice the weight of the giant salamander meat and bone, Under the pressure of 0.2 MPa, cook at 132°C for 60 minutes under high pressure, discharge the bone residue, and obtain giant salamander bone broth;

[0068] Step 2, magnetizing the giant salamander bone broth, and then adding the first enzyme preparation, the second enzyme...

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Abstract

The invention discloses a small molecule peptide extracted from giant salamander meat and bone and an extraction method thereof. The extraction method comprises the following steps: carrying out magnetization on giant salamander bone soup, then adding a first enzyme preparation, a second enzyme preparation and a third enzyme preparation into the magnetized giant salamander bone soup in a staging mode in sequence for carrying out enzyme shearing of peptide chains so as to obtain an enzyme shearing solution; finally, adding the enzyme shearing solution into a protein separation solution, filtering the protein separation solution to obtain a supernate after centrifugal separation, adopting ethyl alcohol to activate the supernate, and finally cooling for centrifugal separation to obtain giantsalamander small molecule peptides. Therefore, according to the method, enzyme shearing of peptide chains is carried out on the magnetized giant salamander bone soup directly, the firm peptide chainsare cracked and partially fall off in the magnetization process, and the magnetized giant salamander bone soup is matched with the first enzyme preparation, the second enzyme preparation and the thirdenzyme preparation, the operability is high, the extraction efficiency for micro-molecule polypeptides reaches up to 2.77%, the enzyme shearing of the peptide chains is quickened, and simultaneouslythe using amounts of the enzyme preparations are reduced, so that the pollution caused by enzymolysis is reduced, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of biological peptide separation, and in particular relates to a small molecule peptide extracted from giant salamander meat and bone and an extraction method thereof. Background technique [0002] The peptide bond is formed by the condensation of the amino group of one amino acid and the carboxyl group of another amino acid. The amide group formed is called a peptide bond in protein chemistry; the molecule of amino acid is the smallest, and the protein is the largest. Peptide bonds form a peptide, and multiple peptides undergo multi-stage folding to form a protein molecule. Proteins are sometimes called "polypeptides"; hundreds of peptides have been found in organisms, which are essential for the body to complete various complex physiological activities Few participants; Among many peptides, small molecular active peptides are easy to absorb, do not increase the burden of human digestion, have extremely hig...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/14
CPCC07K1/14C07K1/34C12P21/06
Inventor 吉建国刘艾平夏豫忠杨斌
Owner 湖北世厚耀榜纪田农业发展有限公司