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System For Automated Explant Preparation And Method Of Use

A technology of explants and systems, applied in the field of seed devices, can solve problems such as difficulty in repeating, effective transformation and regeneration of soybean explants, etc.

Inactive Publication Date: 2018-02-09
DOW AGROSCIENCES LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, soybeans have proven to be a challenging system for genetically engineered
Efficient transformation and regeneration of soybean explants is difficult and often reproducible

Method used

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  • System For Automated Explant Preparation And Method Of Use
  • System For Automated Explant Preparation And Method Of Use
  • System For Automated Explant Preparation And Method Of Use

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Embodiment Construction

[0047] While the present disclosure is susceptible to various modifications and alternative forms, specific exemplary embodiments have been described in detail in the drawings by way of example, and will be described in detail herein. It should be understood, however, that there is no intent to limit the disclosed concepts to the particular forms disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and modifications falling within the spirit and scope of the invention as defined by the appended claims. alternative plan.

[0048] As used herein, the terms "grasp" and "grip" refer to holding or grasping an explant, such as a soybean seed explant or a rapeseed hypocotyl segment, with a tool. Any subsequent mechanism or action that allows the explant to be securely clamped is considered within the scope of the term "grasping".

[0049] As used herein, the term "genetic modification" or "transgene" relates to a plant cell, plant tissue, plant p...

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PUM

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Abstract

A system and method for the automated or semi-automated preparation of explants for transformation and transgenic engineering.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit under 25 USC §119(e) of US Patent Application Serial No. 62 / 186,059, filed June 29, 2015, the entire disclosure of which is incorporated herein by reference. technical field [0003] The present disclosure relates generally to devices for preparing seeds for use in plant breeding, and more particularly to devices for preparing explants for genetic transformation and transgenic engineering. Background technique [0004] Soybean (Glycine max) is one of the most important agricultural crops with an annual crop production of more than 200 million metric tons and an estimated value of more than $40 billion worldwide. Soybeans account for more than 97% of all oilseed production worldwide. Therefore, reliable and effective methods for improving the quality and yield of this valuable crop are of significant interest. [0005] Traditional breeding methods for improving soybeans have been li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B25J9/02C12M1/22C12M1/32C12N5/04G01N1/38
CPCC12M23/50C12M33/00G01N35/0099G01N2035/00524A01H4/001C12N15/8205C12M23/10C12M27/16A01H4/002
Inventor D·L·麦卡蒂二世S·R·契纳雷迪T·齐察克W·E·纪D·巴杜尔J·隆德R·萨里亚P·莫拉比托
Owner DOW AGROSCIENCES LLC