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A method for extracting rifamycin b

A technology of rifamycin and extraction method, which is applied in the field of chemistry, can solve problems such as low efficiency and cumbersome process, and achieve the effects of saving labor costs, improving extraction efficiency, and saving a lot of costs

Active Publication Date: 2020-02-07
南京久安源环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] The technical problem to be solved in the present invention is to provide a method for extracting rifamycin B, so as to solve the problems of low efficiency and complicated process in the prior art

Method used

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  • A method for extracting rifamycin b

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Experimental program
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Effect test

Embodiment 1

[0056] Such as figure 1 Shown, the method for rifamycin B extraction of the present invention comprises the following steps:

[0057] A. Ceramic membrane microfiltration: filter and clarify the rifamycin B-producing fermentation broth or rifamycin B fermentation broth plate and frame filtrate through ceramic microfiltration membranes to remove bacteria and cell debris in the fermentation broth or plate and frame filtrate and impurities such as macromolecular proteins, the obtained microfiltration membrane supernatant contains a large amount of rifamycin B;

[0058] B. Organic scroll ultrafiltration membrane filtration: use ultrafiltration membrane to filter ceramic microfiltration membrane clear liquid, and further remove small molecular proteins in microfiltration membrane clear liquid;

[0059] C. Concentration of organic roll-type nanofiltration membrane: the supernatant of ultrafiltration membrane is concentrated through organic roll-type nanofiltration membrane to obtain...

Embodiment 2

[0075] Such as figure 1 Shown, the method for rifamycin B extraction of the present invention comprises the following steps:

[0076] A. Ceramic membrane microfiltration: filter and clarify the rifamycin B-producing fermentation broth or rifamycin B fermentation broth plate and frame filtrate through ceramic microfiltration membranes to remove bacteria and cell debris in the fermentation broth or plate and frame filtrate and impurities such as macromolecular proteins, the obtained microfiltration membrane supernatant contains a large amount of rifamycin B;

[0077] B. Organic scroll ultrafiltration membrane filtration: use ultrafiltration membrane to filter ceramic microfiltration membrane clear liquid, and further remove small molecular proteins in microfiltration membrane clear liquid;

[0078] C. Concentration of organic roll-type nanofiltration membrane: the supernatant of ultrafiltration membrane is concentrated through organic roll-type nanofiltration membrane to obtain...

Embodiment 3

[0094] Such as figure 1 Shown, the method for rifamycin B extraction of the present invention comprises the following steps:

[0095]A. Ceramic membrane microfiltration: filter and clarify the rifamycin B-producing fermentation broth or rifamycin B fermentation broth plate and frame filtrate through ceramic microfiltration membranes to remove bacteria and cell debris in the fermentation broth or plate and frame filtrate and impurities such as macromolecular proteins, the obtained microfiltration membrane supernatant contains a large amount of rifamycin B;

[0096] B. Organic scroll ultrafiltration membrane filtration: use ultrafiltration membrane to filter ceramic microfiltration membrane clear liquid, and further remove small molecular proteins in microfiltration membrane clear liquid;

[0097] C. Concentration of organic roll-type nanofiltration membrane: the supernatant of ultrafiltration membrane is concentrated through organic roll-type nanofiltration membrane to obtain ...

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Abstract

The invention discloses a method of extracting rifamycin B, comprising the specific steps of 1, filtering fermentation broth or plate-frame filtrate via a ceramic micro-filtration membrane, and clarifying; 2, filtering micro-filtration membrane supernatant with an ultra-filtration membrane to remove impurities; 3, concentrating ultra-filtration supernatant with a nano-filtration membrane; 4, adjusting pH of nano-filtration membrane concentrate to 2.0-2.5, and adding butyl acetate for extracting; 5, adjusting pH of the butyl acetate to 7.5-8.5, and performing two-stage cross-current reversed extraction; 6, adjusting pH of obtained aqueous phase to 2.0-2.5, adding ethyl acetate for secondary extraction; 7, freezing ethyl acetate to separate out crystal; 8, centrifuging to obtain rifamycin Bcrystal; 9, subjecting centrifuged mother liquid to vacuum distillation and crystallization to obtain crude rifamycin B, and returning to the ethyl acetate extracting step. The method has the advantages that mass impurities are removed, the product purity is increased, the yield and unit titer are increased, production steps are decreased, and production cost is decreased; the method is simple toperform and energy efficient, and purity of the rifamycin B finally obtained is greater than 98%.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a method for extracting rifamycin B. Background technique [0002] Rifamycin B (rifamycins) is a complex isolated and extracted from the metabolites of Streptomyces mediterranoi by Sensi et al. in 1959, and then A, B, C, D, E, etc. were separated. Rifamycin B antibiotics have a broad-spectrum antibacterial effect, and have strong effects on Gram-positive bacteria such as Mycobacterium tuberculosis, Leprae, Streptococcus, and pneumococcus, especially drug-resistant Staphylococcus aureus. Also effective against some Gram-negative bacteria. [0003] The core structure of rifamycin B drugs is a macrocyclic lactam with 27 carbon atoms, and the ring contains a naphthalene nucleus. On the naphthalene ring, an aliphatic chain is connected to the C atoms of C-2 and C-12 respectively to form an "Ansa bridge" structure, so it is also classified as ansamycin antibiotics. The C-15 carb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D498/08
CPCC07D498/08Y02P20/10
Inventor 陈磊刘怀红张南南李国学
Owner 南京久安源环保科技有限公司
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