Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for extracting β-ecdysone from Antarctic krill and high-efficiency thin-layer chromatography scanning detection method

A technology of ecdysone and Antarctic krill, which is applied in the food and chemical industry, can solve the problems of increasing the development and utilization value of Antarctic krill, low content of β-ecdysone, and high price of ELISA, and achieves a high degree of automation and analysis process Fast, fully extracted results

Inactive Publication Date: 2020-06-30
SHANDONG NORMAL UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many studies on β-ecdysone in plants, and β-ecdysone is first discovered in dew grass, and its content is abundant in plants. As CN103788162 A discloses a method for extracting ecdysone from spinach, Xu Lirong discloses The extraction, purification process and quality standard of β-ecdysterone in Lushuicao, Wang Qiang and others disclosed the content determination of β-ecdysterone in 19 kinds of resource plants; for the extraction of β-ecdysterone in plants, boiling combined with Ethanol extraction, and degreasing and purification by column chromatography or petroleum ether, the extraction steps are complicated and time-consuming, and for the detection of β-ecdysone in plants, because of its high content, the detection method of thin-layer chromatography is often used, but there are The pre-processing is cumbersome, the thin-layer spots are not obvious, it takes a long time, and the accuracy of the results is low.
There are many reports on β-ecdysone in animals in decapod insects and crustaceans. Most of them use animal serum or lymph fluid, and then extract, purify, and dissolve with the prepared lysate for enzyme-linked immunoassay or high-efficiency Liquid-phase detection, the operation is more complicated, and the enzyme-linked immunosorbent immunoassay is expensive and time-consuming; because the content of β-ecdysone in animals is very low, there is no report of detection by thin-layer chromatography
[0005] At present, there is no report about the method for extracting β-ecdysone from Antarctic krill and the analysis and detection of it. Therefore, it is necessary to establish a method for extracting β-ecdysone from Antarctic krill and an effective detection and analysis method in order to increase Development and Utilization Value of Antarctic Krill

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for extracting β-ecdysone from Antarctic krill and high-efficiency thin-layer chromatography scanning detection method
  • A method for extracting β-ecdysone from Antarctic krill and high-efficiency thin-layer chromatography scanning detection method
  • A method for extracting β-ecdysone from Antarctic krill and high-efficiency thin-layer chromatography scanning detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Extraction of Antarctic krill β-ecdysone and detection by high-efficiency thin-layer chromatography

[0045] 1. Extraction of Antarctic krill β-ecdysone

[0046] (1) Take 100 g of freeze-dried Antarctic krill and pulverize it with a high-speed tissue pulverizer.

[0047] (2) Take crushed Antarctic krill (W) and methanol (V) at a ratio of 1:10-15 for ultrasonic extraction at 35°C, 300W, 40kHz for 1 hour, filter the extract, and collect the filtrate.

[0048] (3) Evaporate the filtrate to dryness in a rotary evaporator at a temperature of 50° C. to obtain a crude extract of β-ecdysone.

[0049] (4) Dissolve the crude extract with 15mL N'N-dimethylimide, centrifuge at 3000r / min, take the supernatant, repeat three times, and combine the supernatant.

[0050] (5) Transfer the supernatant to a rotary evaporator, add absolute ethanol, and evaporate to dryness by rotary evaporation, and dissolve the remaining substance with 10 mL of methanol. Used as a sample solu...

Embodiment 2

[0075] Example 2: Extraction of Antarctic krill β-ecdysone and detection by high-efficiency thin-layer chromatography

[0076] Take 100g of freeze-dried Antarctic krill, pulverize it with a high-speed tissue grinder, add 1000mL of methanol, and extract it by ultrasonic heating in a 35°C, 300w, 40kHz ultrasonic cleaner for 1 hour, filter the extract, and collect the filtrate. The filtrate was evaporated to dryness in a rotary evaporator at a temperature of 50°C to obtain a crude extract of β-ecdysone. Dissolve the crude extract with 15mL N’N-dimethylimide, centrifuge at 3000r / min, take the supernatant, repeat three times, and combine the supernatants. Transfer the supernatant to a rotary evaporator, add absolute ethanol, evaporate to dryness by rotary evaporation, and dissolve the remaining substance with 10 mL of methanol to obtain a sample solution of Antarctic krill β-ecdysone.

[0077] Accurately weigh 1.000mg of β-ecdysone standard substance and dissolve it in 1.0mL of me...

Embodiment 3

[0079] Example 3: Extraction of Antarctic krill β-ecdysone and detection by high-efficiency thin-layer chromatography

[0080] Take 100g of freeze-dried Antarctic krill, pulverize with a high-speed tissue grinder, add 1500mL of methanol, heat and extract in an ultrasonic cleaner at 35°C, 300w, 40kHz, extract for 1 hour, filter the extract, and collect the filtrate. The filtrate was evaporated to dryness in a rotary evaporator at a temperature of 55°C to obtain a crude extract of β-ecdysone. Dissolve the crude extract with 15mL N’N-dimethylimide, centrifuge at 3000r / min, take the supernatant, repeat three times, and combine the supernatants. Transfer the supernatant to a rotary evaporator, add absolute ethanol, evaporate to dryness by rotary evaporation, and dissolve the remaining substance with 10 mL of methanol to obtain a sample solution of Antarctic krill β-ecdysone.

[0081] Accurately weigh 1.000mg of β-ecdysone standard substance and dissolve it in 1.0mL of methanol to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method extracting beta-ecdysone from euphausia superba and an HPTLC (high performance thin layer chromatography) scanning detection method and belongs to the technical fieldof extraction and separation of ecdysone substances. The method is used for extracting beta-ecdysone from the euphausia superba by adopting ethanol as an extraction solvent for extracting beta-ecdysone from the euphausia superba and DMF (dimethyl formamide) a redissolving solvent, the extraction is complete, efficiency is high, besides, the extraction process is simple and fast, and impurities affecting detection can be removed; the HPTLC scanning detection method is utilized for analyzing content of beta-ecdysone in the euphausia superba and is high in automation degree and sensitivity, fewerreagents are required, the analysis process is fast, the result accuracy is high, and the purpose of fast and accurate detection is achieved while the environment is protected very well. The extraction method for beta-ecdysone in the euphausia superba and the effective detection and analysis method are proposed for the first time, and development and utilization value of the euphausia superba isextended.

Description

technical field [0001] The invention belongs to the technical field of food and chemical industry, relates to the technical field of extraction and separation of ecdysone substances, in particular to a method for extracting β-ecdysone from Antarctic krill and a high-efficiency thin-layer chromatography scanning detection method. Background technique [0002] β-ecdysterone, also known as β-ecdysterone, 20-hydroxyecdysterone; molecular formula is C 27 h 44 o 7 , with a molecular weight of 480.63 Daltons, is a light yellow crystalline powder. In animals and plants, β-ecdysone participates in the regulation of many aspects such as molting, reproduction, lifespan, diapause and immune response, and plays an important role in maintaining basic physiological functions. β-ecdysone acts on the human body to promote cell growth and division, accelerate collagen synthesis, stimulate dermal cell division, and promote skin regeneration. At the same time, a large number of animal exper...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/14G01N30/90G01N30/94
CPCG01N30/06G01N30/14G01N30/90G01N30/94
Inventor 韩香凝刘代成
Owner SHANDONG NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com