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Subculture medium conducive to cockscomb callus growth and anthocyanidin accumulation

A technology of subculture medium and callus, which is applied in the field of plant cultivation and subculture of plant callus to achieve the effect of increasing anthocyanin content and yield

Inactive Publication Date: 2018-03-02
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Research data show that the synthesis of anthocyanins requires different types and concentrations of growth regulators in different plant tissues, so the selection of growth regulator types and concentrations directly affects the cultivation of plant tissues by the medium, and there is still no such agent on the market. A subculture medium that can effectively promote the growth of callus and anthocyanin accumulation of Celosia celosiae

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  • Subculture medium conducive to cockscomb callus growth and anthocyanidin accumulation
  • Subculture medium conducive to cockscomb callus growth and anthocyanidin accumulation
  • Subculture medium conducive to cockscomb callus growth and anthocyanidin accumulation

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Embodiment Construction

[0017] The following describes the present invention in detail with reference to embodiments, so as to facilitate the understanding of the present invention by those skilled in the art. It is necessary to point out here that the embodiments are only used to further illustrate the present invention and cannot be understood as limiting the scope of protection of the present invention. Those skilled in the art will make non-essential improvements and improvements to the present invention based on the above-mentioned content of the invention. The adjustment should still belong to the protection scope of the present invention. At the same time, the raw materials mentioned below that are not described in detail are all commercially available products; the process steps or preparation methods not mentioned in detail are all process steps or preparation methods known to those skilled in the art.

[0018] A subculture medium that is conducive to the growth of celosia callus and the accumu...

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Abstract

The invention discloses a subculture medium conducive to cockscomb callus growth and anthocyanidin accumulation; the subculture medium includes a B5 basic culture medium and a compound growth regulator, wherein the composite growth regulator includes 2,4-D with the concentration of 0.2 mg / L. The B5 culture medium is used as the basic culture medium, and the 2,4-D with the concentration of 0.2 mg / Lis added in the composite growth regulator, so the subculture medium can effectively promote cockscomb callus growth and increase the anthocyanidin accumulation amount in cockscomb calluses in the subculture process, thereby greatly improving the anthocyanidin content and yield of the cockscomb calluses.

Description

Technical field [0001] The invention relates to the field of plant cultivation, in particular to the field of subculture of plant callus. Background technique [0002] Anthocyanins are a class of substances widely present in plant tissues. Because of their bright colors and beneficial to human health, they have long been one of the important natural pigments that people research, develop and use. The commonly used anthocyanins are all extracted from plant tissues, and their raw material sources and yields are restricted. The method of cultivating plant cells to produce anthocyanins can avoid occupying a large amount of land, and at the same time, it is not restricted by seasons, and the yield can be increased. Therefore, people have paid attention to it in recent years. [0003] The key to using cell engineering technology to carry out the secondary metabolism of anthocyanins required for industrial production is to establish a high-yield and stable cell line. In addition to inte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 王惠珍陈萍文锦欢钟希琼何丽烂
Owner FOSHAN UNIVERSITY