Method for extracting andrographolide in andrographis tablets

Abstract

The invention discloses a method for extracting andrographolide in andrographis tablets. The method comprises the following steps: taking a proper amount of andrographis tablets, crushing the tablets,putting the mixture into an enzymolysis tank, adding complex enzyme according to the mass ratio, carrying out vortex mixing, carrying out enzymolysis at 30-50 DEG C for 1-2 hours, filtering to obtainan enzymatic hydrolysate; and rapidly freezing the obtained enzymatic hydrolysate and then naturally unfreezing, so that starch in the extract is aged and precipitated; collecting a supernatant, subjecting the residual part to centrifuging, collecting the centrifugal liquid and combining the centrifugal liquid with the supernatant, carrying out spray drying, and carrying out CO2 supercritical extraction on the obtained powder, wherein the fluid extraction pressure is 20-30 MPa, the extraction temperature is 40-50 DEG C, the CO2 fluid flow is 750-850 L/h, the extraction time is 3-5 hours; collecting the andrographolide. The andrographolide can be efficiently extracted from the andrographis tablets, the operation is convenient, and the impurities in the extracting solution are not increased, expensive equipment is not needed, and the extraction cost of andrographolide is reduced.

Description

technical field [0001] The invention relates to the field of chemical extraction, in particular to a method for extracting andrographolide in Chuanxinlian tablets. Background technique [0002] Chuanxinlian tablet is a heat-clearing agent, which has the effects of clearing heat and detoxifying, cooling blood and reducing swelling. It is mainly used to treat pathogenic toxins, cold and fever, sore throat, sores of the mouth and tongue, sudden cough and overworked cough, diarrhea and dysentery, astringent pain due to hot stranguria, carbuncle sores, and poisonous snake bites. The main ingredient is Andrographis paniculata, and the auxiliary materials are starch and magnesium stearate. There are four lactone components in andrographolide: deoxyandrographolide, andrographolide, neoandrographolide and dehydroandrographolide. Andrographolide and total lactones are clinically commonly used ingredients. Its main pharmacological activities are anti-allergic, anti-HIV virus, anti-i...

AI Technical Summary

Problems solved by technology

[0003] At present, there are many reports on the extraction methods of andrographolide, including water extraction, alcohol extraction, alkaline water extraction, etc. These processes are simple to operate, but have low extraction efficiency, low raw material utilization rate, large solvent usage and Impurities are difficult to remove, and other extraction methods, such as supercritical extraction, ultrasonic-assisted extraction, microwave-assisted extraction, resin adsorption, etc., although high-purity andrographolide can be obtained, the required equipment is expensive and greatly Increased production costs, and difficult to apply to industrial mass production