Extraction method and application of ginsenoside Rg5 and ginsenoside Rg1

A technology of ginsenosides and extraction methods, applied in the field of medicine, to achieve the effect of improving or relieving symptoms and increasing content

Inactive Publication Date: 2018-03-13
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports of the effects of ginsenoside Rk1 on the nervous system

Method used

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  • Extraction method and application of ginsenoside Rg5 and ginsenoside Rg1
  • Extraction method and application of ginsenoside Rg5 and ginsenoside Rg1
  • Extraction method and application of ginsenoside Rg5 and ginsenoside Rg1

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Experimental program
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Effect test

preparation example Construction

[0039] Preparation of the reference solution: Accurately weigh ginsenoside Rg 55.19 mg and 5.23 mg of ginsenoside Rk1 were placed in a 5 mL volumetric flask, dissolved with methanol and the volume was adjusted to the mark, and shaken well.

[0040] The structural diagrams of monomeric saponins ginsenosides Rb1, Rg5 and Rk1 are shown in figure 1 shown, from figure 1 The structures of the monomeric saponins ginsenosides Rb1, Rg5 and Rk1 are known.

Embodiment 1

[0041] Embodiment 1: The raw material of Panax ginseng in this embodiment is dried notoginseng roots, powdered, and passed through a 40-mesh sieve;

[0042] A method for extracting ginsenoside Rg5 and ginsenoside Rk1, the specific steps are as follows:

[0043] (1) Steam the raw material of Panax notoginseng plant (Notoginseng powder), and then reflux extraction with a solvent (the solvent is ethanol) to obtain the extract, wherein the solid-liquid mixture of the raw material of Panax notoginseng plant (Notoginseng powder) and solvent (ethanol) The ratio kg:L is 1:10, the number of times of reflux extraction is 5 times, and the time of each reflux extraction is 2 h; the method of steaming treatment is to soak the Panax notoginseng powder with water, wrap it with gauze, and place it in a refrigerator at 120°C. Steam in a sterilizing pot for 12 hours;

[0044] (2) Volatilize the solvent (ethanol) in the extract obtained in step (1) to obtain an ethanol extract, dissolve the eth...

Embodiment 2

[0049] Embodiment 2: the present embodiment selects Panax notoginseng saponins as raw material;

[0050] A method for extracting ginsenoside Rg5 and ginsenoside Rk1, the specific steps are as follows:

[0051] (1) Steaming the total saponins of Panax notoginseng; the method of steaming treatment is to suspend the total saponins of Panax notoginseng in water, and steam in a reaction kettle at 105°C for 10 hours;

[0052] (2) Dissolve the steamed product obtained in step (1) in organic solvent D (organic solvent D is ethanol) to obtain an organic solution of total saponins, and the organic solution of total saponins is subjected to macroporous adsorption resin column chromatography, and the organic solvent is eluted with water E (elution organic solvent E is ethanol) for elution, and then eluted with water-containing organic solvent F (elution organic solvent F is ethanol) until no saponin flows out to obtain grouped saponins with a total content of ginsenoside Rg5 and Rk1 great...

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Abstract

The invention relates to an extraction method and application of ginsenoside Rg5 and ginsenoside Rg1 and belongs to the technical field of medicine. The extraction method includes: using a solvent toperform reflux extraction on steamed panax plant raw materials, subjecting the extract to macroporous resin column chromatography to obtain total saponins containing the ginsenoside Rg5 and the ginsenoside Rg1, subjecting the total saponins, steamed total ginsenoside, total panax notoginseseng saponins or steamed ginsenoside Rg1 to macroporous resin column chromatography to obtain group saponins containing with the total content of the ginsenoside Rg5 and the ginsenoside Rg1 being larger than 97%, and subjecting the group saponins to macroporous resin column chromatography to obtain the singleginsenoside Rg5 with the purity being larger than 90% and the single ginsenoside Rg1 with the purity being larger than 90%. The total saponins, the group saponins, the single ginsenoside Rg5 and thesingle ginsenoside Rg1 extracted by the method can evidently improve the motion ability and response ability of zebrafish in an aluminum trichloride-induced Alzheimer's disease zebrafish model.

Description

technical field [0001] The invention relates to an extraction method and application of ginsenoside Rg5 and ginsenoside Rk1, belonging to the technical field of medicine. Background technique [0002] Panax notoginseng and ginseng, American ginseng and other ginseng plants contain similar chemical components, including saponins, flavonoids, polyacetylenic alcohols, polysaccharides, etc., and dammarane-type tetracyclic triterpene saponins are one of the main physiologically active components of ginseng medicinal plants . So far, it has been found that Panax notoginseng saponins and some monomeric compounds have good pharmacological activities in the cardiovascular system, nervous system, substance metabolism, anti-inflammation, anti-tumor, etc., especially the activity in the nervous system has been reported more and more more. For example, Panax notoginseng saponins and monomeric saponins Rb1 and Rg1 can significantly enhance the learning and memory ability of mice; Panax ...

Claims

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Application Information

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IPC IPC(8): C07J17/00A61K31/704A61P25/28
CPCA61K31/704C07J17/005
Inventor 许敏吴涛韩佳欣杨崇仁
Owner KUNMING UNIV OF SCI & TECH
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