An intravenous injection for enhancing immune function
A technology for enhancing immunity, injections, applied in the field of biomedicine
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Embodiment 1
[0030] Inoculate Enterococcus faecium (purchased from China Industrial Microorganism Culture Collection Management Center, Latin name: Enterococcusfaecium, storage number: CICC 6049) in MRS medium, culture in 37°C incubator for 24 hours, then centrifuge at 3000 rpm for 5 minutes, remove Keep the precipitate in the upper layer culture solution, add sterile physiological saline to wash the precipitate, centrifuge for 5 minutes, repeat the washing 3 times, add sterile physiological saline, mix with the precipitate, and make a suspension. Get a certain amount of Enterococcus faecium physiological saline suspension, measure its OD value at 690nm place of the spectrophotometer, when the OD value of the final concentration diluted with sterile physiological saline is 0.38, the Enterococcus faecium physiological The salt suspension is used as 1 times (1×) concentration, and the bacterial count can be obtained by THOMA bacterial counting plate. Under the condition of this concentration,...
Embodiment 2
[0032]Inoculate Enterococcus faecium (purchased from China Industrial Microorganism Culture Collection Management Center, Latin name: Enterococcusfaecium, storage number: CICC 6049) in MRS medium, culture in 37°C incubator for 24 hours, then centrifuge at 3000 rpm for 5 minutes, remove The supernatant culture solution retains the precipitate, adds 0.9% sterile physiological salt to wash the precipitate, centrifuges for 5 minutes, repeats the washing 3 times, adds an appropriate amount of 0.9% sterile normal saline, and mixes with the precipitate. Get a certain amount of lactic acid bacteria suspension, measure its OD value at spectrophotometer 690nm place, when the OD value of the final concentration diluted with sterilized physiological saline is 0.38, the lactic acid bacteria suspension of such dilution concentration is regarded as 1 times ( 1×) concentration. Based on this, the lactic acid bacteria suspensions with 5×, 1× and 0.2× concentrations can be obtained by diluting ...
Embodiment 3
[0037] Using the inactivated Enterococcus faecium suspension of 5×, 1× and 0.2× concentrations prepared in Example 2, the carbon particle clearance test method was used to detect the effect of intravenous injection of the inactivated Enterococcus faecium suspension on the immunosuppressive mouse model mononuclear - Effects on macrophage phagocytosis. The clean-grade Kunming mice weighing 18-22 g were divided into normal control group, immunosuppressive model group, thymopentin group and high, medium and low doses of inactivated Enterococcus faecium groups. There were 10 mice in each group, half male and half male. The mice were injected intraperitoneally with dexamethasone at a dose of 40 mg / kg, once a day, for 5 consecutive days to prepare the mouse immunosuppressive model. On the day when the mouse immunosuppressive model was prepared, the normal control group and the immunosuppressive model group were injected with normal saline into the tail vein, and the thymopentin grou...
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