Hypocrellin nanometer preparation for actively targeting specific tumor cells as well as preparation method and application thereof
A technology of oleocanthal and tumor cells, applied in the field of nano-medicine, can solve the problems of poor metabolism, lack of biocompatibility, metabolic burden, etc., to improve encapsulation efficiency, increase targeting and success The effect of high rate and encapsulation rate
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Embodiment 1
[0032] The carboxymethyl chitosan (CMC) that takes 300mg, the polyvinyl alcohol (PVA) of 500mg is dissolved in 50mL ultrapure water, makes aqueous phase; Hypocretin A (HA) was dissolved in 5 mL of acetone as the organic phase, and the organic phase was added dropwise to the aqueous phase stirred at a high speed of 10000 rpm / min. After the dropwise addition, 50W ultrasonic dispersion was performed for 10min, and then placed on a magnetic stirrer and stirred at 1000rpm / min for 12h to evaporate the organic solvent. The remaining solvent is centrifuged at 13000rpm / min to remove unencapsulated HA, unreacted PLGA and CMC, and excess emulsifier, and the resulting precipitate is polylactic acid-glycolic acid / carboxymethyl chitosan encapsulated Hypocretin nanoparticles. Wash the nanoparticles, resuspend them in 50 mL of 0.5M NaCl activation buffer, add 20 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 30 mg of N-hydroxybutyl Imide was activated for 15 minutes, ...
Embodiment 2
[0035] Example 2 has the same raw materials and preparation method as Example 1, except that 100 mg of CMC, 300 mg of PVA, 100 mg of PLGA, 12 mg of hypocrellin and 125 mg of transferrin are used.
[0036] Hypocretin B, 2 mg / mL transferrin, and 0.2 mg / mL hypocretin B in the hypocretin nanoparticles actively targeting specific tumor cells obtained in this example, the solvent is water, and the encapsulation efficiency of hypocretin is 83%. %, the loading of transferrin was 80%.
Embodiment 3
[0038] Example 3 has the same raw materials and preparation method as Example 1, except that 500 mg of CMC, 100 mg of PVA, 500 mg of PLGA, 37.5 mg of Hypocretin C and 25 mg of transferrin are used.
[0039] The hypocretin nano-preparation actively targeting specific tumor cells obtained in this example contains 0.6 mg / mL hypocretin C, 0.5 mg / mL transferrin, and water as a solvent. The encapsulation efficiency of hypocrellin was 82%.
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