Method for detecting influence of electronic cigarette aerosol aqueous extract on cell active oxygen secretion volume

Abstract

The invention discloses a method for detecting influence of an electronic cigarette aerosol aqueous extract on cell active oxygen secretion volume. The method includes the steps of sample pretreatment, single cell suspension preparation, cell concentration calculation, cell inoculation, tested substance exposure, probe loading, sample determination, active oxygen level calculation and the like. Anexposure way and an action mode of electronic cigarette products are comprehensively investigated, a brand-new electronic cigarette product smoke pretreatment method is established, human pulmonary fibroblasts HPF are selected as cells for detection, and the sample detection dosage is determined. With the effective treatment of the sample, the optimal setting of the detection dosage and the correct selection of target cells, the method can accurately detect the influence of the electronic cigarette products on the cell active oxygen secretion volume, and then can effectively evaluate oxidative damage of the electronic cigarette products to the cells.

Description

technical field [0001] The invention belongs to the technical field of biological safety evaluation of tobacco and tobacco products, and in particular relates to a method for detecting the influence of electronic cigarette aerosol water extract on the secretion of active oxygen in cells. Background technique [0002] As people pay more and more attention to health, higher requirements are put forward for tobacco products, not only to have a better taste, but also to reduce the adverse feelings of the human body as much as possible. E-cigarette products avoid the harm caused by the complex mixture produced by the combustion of traditional cigarette products. At this stage, it has become one of the new directions for foreign tobacco companies to shift from traditional cigarette products. During the use of electronic cigarette products, the liquid composed of nicotine, flavoring substances, etc. is converted into a mixture similar to cigarette smoke by electric heating or elect...

AI Technical Summary

Problems solved by technology

However, the existing technology usually directly analyzes and tests the e-cigarette liquid itself, without considering its shape, migration and other processes during smoking, which leads to errors in the results of the analysis and test, and cannot accurately reflect the amount of reactive oxygen species secreted by the e-cigarette liquid. the true impact of

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