Method for detecting influence of total particulate matter of heat-not-burn cigarette on catalase activity of cells
A heat-not-burn and catalase technology, applied in biochemical equipment and methods, measurement devices, biomaterial analysis, etc., can solve the problems of no uniform standard method, time-consuming and labor-intensive
Inactive Publication Date: 2019-04-12
CHINA TOBACCO YUNNAN IND
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Problems solved by technology
However, there are a large number of primary formulations, and sensory evaluation of all of them is time-consuming and labor-intensive, and the sensory evaluation focuses on screening the style and taste of the product. How to use biological testing technology to screen the primary formulations of the product to obtain the results of reducing the adverse feelings of the human body? Product formula is still in the exploratory stage, and there is no unified standard method
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Embodiment 1
[0037] Prepare 3 kinds (1#, 2#, 3#) of heat-not-burn cigarettes with total particulate matter captured by Cambridge filter as samples, and test its effect on cell catalase activity.
[0038] The specific detection process includes the following steps:
[0039] (1) Sample pretreatment: using a linear smoking machine, under the suction conditions of a suction volume of 55.0mL, a duration of 3s, and a suction frequency of 30s, continuously suck 10 mouthfuls, and use a Cambridge filter with a diameter of 44mm Capture, weigh the mass of the smoke trap before and after smoking, and calculate the mass mTPM of the total particulate matter in the heat-not-burn cigarette according to formula (1);
[0040] mTPM=m 1 -m 0 (1)
[0041] In the formula:
[0042] m 0 - the mass of the smoke trap before suction, in milligrams (mg);
[0043] m 1 - the mass of the smoke trap after suction, in milligrams (mg);
[0044]After the capture is completed, put the captured Cambridge filter into ...
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Abstract
The invention discloses a method for detecting influence of total particulate matter of heat-not-burn cigarette on catalase activity of cells. The method comprises the following steps of (1) pretreating samples; (2) preparing human nasal cavity epithelial cell unicellular suspension liquid; (3) calculating the cell concentration of the human nasal cavity epithelial cell unicellular suspension liquid; (4) performing inoculation on the human nasal cavity epithelial cell unicellular suspension liquid; (5) enabling test substances to be exposed; (6) collecting cells; (7) performing cell lysis; (8)compounding a standard solution; (9) compounding samples; (10) performing absorbance determination; (11) performing protein concentration determination; and (12) calculating the enzyme activity of catalase. According to the method disclosed by the invention, through effective treatment on the samples, optimal setting of detection dosage and right selection of target cells, the method can accurately detect the influence of the total particulate matter of the heat-not-burn cigarette on catalase activity, and further oxidative damage of the total particulate matter of the heat-not-burn cigaretteon the cells can be effectively evaluated.
Description
technical field [0001] The invention belongs to the technical field of safety biological effect evaluation of tobacco products, and particularly relates to a method for detecting the influence of total particulate matter in heat-not-burn cigarettes on cell catalase activity. Background technique [0002] As people pay more and more attention to health, higher requirements are put forward for tobacco products, not only to have a better taste, but also to reduce the adverse feelings of the human body as much as possible. Heat-not-burn cigarette products avoid the harm caused by the complex mixture produced by the combustion of traditional cigarette products. At this stage, it has become one of the new directions for foreign tobacco companies to shift from traditional cigarette products. [0003] Heat-not-burn cigarettes are characterized by heating tobacco rather than burning it, providing consumers with a certain tobacco characteristic experience. In the initial stage of pro...
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IPC IPC(8): C12Q1/30
CPCC12Q1/30G01N2333/908
Inventor 管莹李雪梅汤建国朱东来张霞韩敬美何东沅张伟高茜徐玉琼
Owner CHINA TOBACCO YUNNAN IND